Method of and an arrangement for cultivating cells of animal and human tissues

Claim 1

An arrangement for cultivating cells of animal and human tissues which are highly susceptible to damage resulting from agitation, comprising an upright container which bounds an unobstructed inner chamber for nutrient solution containing the cells which tend to settle in the solution; guide means located in said inner chamber of said container and subdividing the interior of the latter into two unobstructed zones; means for accommodating an oxygen-containing gas and including an outer gas chamber located below said container; and means for introducing said oxygen-containing gas from said outer gas chamber of said accommodating means into said inner chamber of said container, said introducing means including a plate separating said outer gas chamber from said inner chamber of said container and having a plurality of outlet openings communicating said outer gas chamber with said inner chamber so as to introduce said oxygen-containing gas from the former into the latter, wherein the number and the dimensions of said outlet openings are such that when said oxygen-containing gas is introduced from said outer gas chamber into said inner chamber of said container through said plurality of outlet openings of said plate, it imparts to said nutrient solution in said inner chamber a circulatory movement between said unobstructed zones which is sufficient to prevent the cells from settling and maintain them in suspended state, but is so slight as to avoid agitating said nutrient solution with concomitant damage to the cells, said introducing means constituting the only means for imparting circulatory movement to said nutrient solution and maintaining the cells in suspended state.