US4311686A
Expired / Lapsed
US
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Methodology for the immunodiagnosis of multiple sclerosis and/or malignant diseases from blood sample analysis
Assignee: Immunology Development Corp
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Filing: 1979-04-30
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Granted: 1982-01-19
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Expires: 1999-04-30
Abstract
Abstract New and improved methodology for the diagnosis of a disease from blood sample analysis is disclosed and comprises: the preparation of a putative antigenic body substance extract, which is specific to disease-sensitized blood leukocytes, from the pooled, like body substances of a plurality of donors known to have the disease of interest; the mixture of said extract with the blood sample leukocytes; the promotion of the reaction therebetween to modify a characteristic of the disease-sensitized leukocytes, if any, of the blood sample; and the determination of the extent, if any, to which said characteristic has been modified. In multiple sclerosis diagnosis, said body substance may be blood or urine; while for malignant disease (for example breast cancer, or cancer of the head and neck) said body substance may be blood, urine, pleural fluid, ascites fluid, supernates of tumor cells or tumor cells grown in culture media.
Inventors
- 👤 John W. Angers
- 👤 Lazar Korik
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 22:40
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/0005; A61K39/0008; A61K39/0011; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/564; G01N2800/00; G01N2800/28; G01N2800/285; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S530/827; Y10S530/829
Claims Analysis
Total Claims: 72
Claim 1
Independent Claim
78 words
A method for the diagnosis of multiple sclerosis by blood sample analysis which comprises the steps of mixing a putative antigenic blood extract prepared from multiple sclerosis-sensitized blood and specific to multiple sclerosis-sensitized blood leukocytes with leukocytes of the blood sample and promoting the reaction therebetween to inhibit the ability of the multiple sclerosis-sensitized leukocytes of the blood sample to adhere to a glass surface, and determining the extent to which said leukocyte adherence ability has been inhibited.
Claim 2
Independent Claim
78 words
A method for the diagnosis of multiple sclerosis by blood sample analysis which comprises the steps of mixing a putative antigenic blood extract prepared from multiple sclerosis-sensitized blood and specific to multiple sclerosis-sensitized blood leukocytes with leukocytes of the blood sample and promoting the reaction therebetween to inhibit the ability of the multiple sclerosis-sensitized leukocytes of the blood sample to adhere to a glass surface, and determining the extent to which said leukocyte adherence ability has been inhibited.
Claim 3
Dependent Claim
25 words
The method of claim 1 further comprising the step of separating out the leukocytes of said blood sample prior to mixing with said blood extract.
Claim 4
Dependent Claim
39 words
The method of claim 1 further comprising the step of pooling the whole bloods of a plurality of donors known to have multiple sclerosis and preparing said blood extract therefrom for mixing with the leukocytes of said blood sample.
Claim 5
Independent Claim
94 words
A method for the diagnosis of multiple sclerosis by blood sample analysis which comprises the steps of labeling the lymphocytes in the blood sample with an energy-emitting material, mixing a putative antigenic blood extract prepared from multiple sclerosis-sensitized blood and specific to multiple sclerosis-sensitized blood leukocytes with the lymphocytes of the blood sample and promoting the reaction therebetween to inhibit the ability of the multiple sclerosis-sensitized lymphocytes of the blood sample to adhere to a glass surface, and determining by energy-emitting material detection the extent to which said lymphocyte adherence ability has been inhibited.
Claim 6
Independent Claim
94 words
A method for the diagnosis of multiple sclerosis by blood sample analysis which comprises the steps of labeling the lymphocytes in the blood sample with an energy-emitting material, mixing a putative antigenic blood extract prepared from multiple sclerosis-sensitized blood and specific to multiple sclerosis-sensitized blood leukocytes with the lymphocytes of the blood sample and promoting the reaction therebetween to inhibit the ability of the multiple sclerosis-sensitized lymphocytes of the blood sample to adhere to a glass surface, and determining by energy-emitting material detection the extent to which said lymphocyte adherence ability has been inhibited.
Claim 7
Dependent Claim
25 words
The method of claim 4 further comprising the step of separating out the lymphocytes of said blood sample prior to mixing with said blood extract.
Claim 8
Dependent Claim
39 words
The method of claim 4 further comprising the step of pooling the whole bloods of a plurality of donors known to have multiple sclerosis and preparing said blood extract therefrom for mixing with the lymphocytes of said blood sample.
Claim 9
Dependent Claim
21 words
The method of claim 4 further comprising the step of labeling the lymphocytes of said blood sample with a radioactive material.
Claim 10
Independent Claim
96 words
A method for the diagnosis of multiple sclerosis by blood sample analysis which comprises the steps of coating agglutination means with a putative antigenic blood extract prepared from multiple sclerosis-sensitized blood and specific to multiple sclerosis-sensitized blood leukocytes to enhance the capability of said agglutination means to cause clumping of multiple sclerosis-sensitized blood leukocytes, contacting the thusly coated agglutination means with the leukocytes of said blood sample and promoting the reaction therebetween to cause clumping of the multiple sclerosis-sensitized leukocytes of the blood sample and determing the extent to which said blood sample leukocytes have clumped.
Claim 11
Independent Claim
96 words
A method for the diagnosis of multiple sclerosis by blood sample analysis which comprises the steps of coating agglutination means with a putative antigenic blood extract prepared from multiple sclerosis-sensitized blood and specific to multiple sclerosis-sensitized blood leukocytes to enhance the capability of said agglutination means to cause clumping of multiple sclerosis-sensitized blood leukocytes, contacting the thusly coated agglutination means with the leukocytes of said blood sample and promoting the reaction therebetween to cause clumping of the multiple sclerosis-sensitized leukocytes of the blood sample and determing the extent to which said blood sample leukocytes have clumped.
Claim 12
Dependent Claim
26 words
The method of claim 8 further comprising the step of separating out the leukocytes of said blood sample prior to contacting thereof with said agglutination means.
Claim 13
Dependent Claim
35 words
The method of claim 8 further comprising the step of pooling the whole bloods of a plurality of donors known to have multiple sclerosis and preparing said blood extract therefrom for coating said agglutination means.
Claim 14
Independent Claim
71 words
A method for the diagnosis of multiple sclerosis by blood sample analysis which comprises the steps of mixing a putative antigenic blood extract prepared from multiple sclerosis-sensitized blood and specific to multiple sclerosis-sensitized blood leukocytes with the leukocytes of the blood sample and promoting the the reaction therebetween to lyse the multiple sclerosis-sensitized leukocytes of the blood sample and determining the extent to which the blood sample leukocytes have been lysed.
Claim 15
Independent Claim
71 words
A method for the diagnosis of multiple sclerosis by blood sample analysis which comprises the steps of mixing a putative antigenic blood extract prepared from multiple sclerosis-sensitized blood and specific to multiple sclerosis-sensitized blood leukocytes with the leukocytes of the blood sample and promoting the the reaction therebetween to lyse the multiple sclerosis-sensitized leukocytes of the blood sample and determining the extent to which the blood sample leukocytes have been lysed.
Claim 16
Dependent Claim
25 words
The method of claim 11 further comprising the step of separating out the leukocytes of said blood sample prior to mixing with said blood extract.
Claim 17
Dependent Claim
32 words
The method of claim 11 further comprising the step of lysing the red blood cells of said blood sample prior to mixing the leukocytes of said blood sample with said blood extract.
Claim 18
Dependent Claim
39 words
The method of claim 11 further comprising the step of pooling the whole bloods of a plurality of donors known to have multiple sclerosis and preparing said blood extract therefrom for mixing with the leukocytes of said blood sample.
Claim 19
Independent Claim
69 words
A method for the preparation of a putative antigenic blood extract specific to multiple sclerosis which comprises the steps of pooling the whole bloods from a plurality of donors known to have multiple sclerosis, placing said pooled whole bloods in solution, concentrating putative antigenic multiple sclerosis-sensitized components present in said pooled whole bloods, separating said concentrated components from said solution, and clarifying said components to form said blood extract.
Claim 20
Independent Claim
69 words
A method for the preparation of a putative antigenic blood extract specific to multiple sclerosis which comprises the steps of pooling the whole bloods from a plurality of donors known to have multiple sclerosis, placing said pooled whole bloods in solution, concentrating putative antigenic multiple sclerosis-sensitized components present in said pooled whole bloods, separating said concentrated components from said solution, and clarifying said components to form said blood extract.
Claim 21
Dependent Claim
19 words
The method of claim 15 wherein the pooled whole bloods placed in solution are mixed with a non-isotonic medium.
Claim 22
Dependent Claim
36 words
The method of claim 15 wherein said putative antigenic multiple sclerosis-sensitized components are concentrated and separated from said solution by centrifuging said solution, dialyzing the resultant supernate, centrifuging the resultant non-dialysate and filtering the resultant supernate.
Claim 23
Dependent Claim
29 words
The method of claim 15 wherein said putative antigenic multiple sclerosis-sensitized components are clarified by placing the separated and concentrated components in isotonic solution and centrifuging said isotonic solution.
Claim 24
Independent Claim
84 words
A method for the preparation of a putative antigenic blood extract specific to multiple sclerosis which comprises the steps of pooling the whole bloods from a plurality of donors known to have multiple sclerosis, placing said pooled whole bloods in non-isotonic solution, concentrating putative antigenic multiple sclerosis-sensitized components present in said pooled whole bloods, separating said concentrated components from said solution, placing the thusly separated and concentrated putative antigenic multiple sclerosis-sensitized components in isotonic solution and clarifying said components to form said blood extract.
Claim 25
Independent Claim
84 words
A method for the preparation of a putative antigenic blood extract specific to multiple sclerosis which comprises the steps of pooling the whole bloods from a plurality of donors known to have multiple sclerosis, placing said pooled whole bloods in non-isotonic solution, concentrating putative antigenic multiple sclerosis-sensitized components present in said pooled whole bloods, separating said concentrated components from said solution, placing the thusly separated and concentrated putative antigenic multiple sclerosis-sensitized components in isotonic solution and clarifying said components to form said blood extract.
Claim 26
Dependent Claim
21 words
A method of claim 19 wherein the whole bloods of at least five donors known to have multiple sclerosis are pooled.
Claim 27
Independent Claim
87 words
A method for the diagnosis of a specific malignant disease of interest by blood sample analysis which comprises the steps of mixing a putative antigenic blood extract prepared from blood sensitized to said malignant disease, said extract being specific to malignant disease-sensitized blood leukocytes, with leukocytes of the blood sample and promoting the reaction therebetween to inhibit the ability of the malignant disease-sensitized leukocytes of the blood sample to adhere to a glass surface and determining the extent to which said leukocyte adherence ability has been inhibited.
Claim 28
Independent Claim
87 words
A method for the diagnosis of a specific malignant disease of interest by blood sample analysis which comprises the steps of mixing a putative antigenic blood extract prepared from blood sensitized to said malignant disease, said extract being specific to malignant disease-sensitized blood leukocytes, with leukocytes of the blood sample and promoting the reaction therebetween to inhibit the ability of the malignant disease-sensitized leukocytes of the blood sample to adhere to a glass surface and determining the extent to which said leukocyte adherence ability has been inhibited.
Claim 29
Dependent Claim
43 words
The method of claim 21 further comprising the step of pooling the whole bloods of a plurality of donors known to have the specific malignant disease of interest and preparing said blood extract therefrom for mixing with the leukocytes of said blood sample.
Claim 30
Independent Claim
98 words
A method for the diagnosis of a specific malignant disease of interest by blood sample analysis which comprises the steps of labeling the lymphocytes in the blood sample with an energy-emitting material, mixing a putative antigenic blood extract prepared from blood sensitized to said malignant disease said extract being specific to malignant disease-sensitized blood leukocytes with the lymphocytes of said blood sample and promoting the reaction therebetween to inhibit the ability of the blood sample to adhere to a glass surface and determining by energy-emitting material detection the extent to which said lymphocyte adherence ability has been inhibited.
Claim 31
Independent Claim
98 words
A method for the diagnosis of a specific malignant disease of interest by blood sample analysis which comprises the steps of labeling the lymphocytes in the blood sample with an energy-emitting material, mixing a putative antigenic blood extract prepared from blood sensitized to said malignant disease said extract being specific to malignant disease-sensitized blood leukocytes with the lymphocytes of said blood sample and promoting the reaction therebetween to inhibit the ability of the blood sample to adhere to a glass surface and determining by energy-emitting material detection the extent to which said lymphocyte adherence ability has been inhibited.
Claim 32
Dependent Claim
43 words
The method of claim 23 further comprising the step of pooling the whole bloods of a plurality of donors known to have the specific malignant disease of interest and preparing said blood extract therefrom for mixing with the lymphocytes of said blood extract.
Claim 33
Independent Claim
105 words
A method for the diagnosis of a specific malignant disease of interest by blood sample analysis which comprises the steps of coating agglutination means with a putative antigenic blood extract prepared from blood sensitized to said malignant disease, said extract being specific to malignant disease-sensitized blood leukocytes, to enhance the capability of said agglutination means to cause clumping of malignant disease-sensitized blood leukocytes, contacting the thusly coated agglutination means with the leukocytes of said blood sample and promoting the reaction therebetween to cause clumping of the malignant disease-sensitized leukocytes of the blood sample and determining the extent to which said blood sample leukocytes have clumped.
Claim 34
Independent Claim
105 words
A method for the diagnosis of a specific malignant disease of interest by blood sample analysis which comprises the steps of coating agglutination means with a putative antigenic blood extract prepared from blood sensitized to said malignant disease, said extract being specific to malignant disease-sensitized blood leukocytes, to enhance the capability of said agglutination means to cause clumping of malignant disease-sensitized blood leukocytes, contacting the thusly coated agglutination means with the leukocytes of said blood sample and promoting the reaction therebetween to cause clumping of the malignant disease-sensitized leukocytes of the blood sample and determining the extent to which said blood sample leukocytes have clumped.
Claim 35
Dependent Claim
39 words
The method of claim 25 further comprising the step of pooling the whole bloods of a plurality of donors known to have the malignant disease of interest and preparing said blood extract therefrom for coating of said agglutination means.
Claim 36
Independent Claim
79 words
A method for the diagnosis of a specific malignant disease of interest by blood sample analysis which comprises the steps of mixing a putative antigenic blood extract prepared from blood sensitized to said malignant disease, said extract being specific to malignant disease-sensitized blood leukocytes, with the leukocytes of said blood sample and promoting the reaction therebetween to lyse the malignant disease-sensitized leukocytes of the blood sample and determining the extent to which the blood sample leukocytes have been lysed.
Claim 37
Independent Claim
79 words
A method for the diagnosis of a specific malignant disease of interest by blood sample analysis which comprises the steps of mixing a putative antigenic blood extract prepared from blood sensitized to said malignant disease, said extract being specific to malignant disease-sensitized blood leukocytes, with the leukocytes of said blood sample and promoting the reaction therebetween to lyse the malignant disease-sensitized leukocytes of the blood sample and determining the extent to which the blood sample leukocytes have been lysed.
Claim 38
Dependent Claim
42 words
The method of claim 27 further comprising the step of pooling the whole bloods of a plurality of donors known to have the malignant disease of interest and preparing said blood extract therefrom for mixing with the leukocytes of said blood sample.
Claim 39
Independent Claim
76 words
A method for the preparation of a putative antigenic blood extract specific to a particular malignant disease of interest which comprises the steps of pooling the whole bloods from a plurality of donors known to have the malignant disease of interest, placing said pooled whole bloods in solution, concentrating putative antigenic malignant disease-sensitized components present in said pooled whole bloods, separating said concentrated components from said solution, and clarifying said components to form said blood extract.
Claim 40
Independent Claim
76 words
A method for the preparation of a putative antigenic blood extract specific to a particular malignant disease of interest which comprises the steps of pooling the whole bloods from a plurality of donors known to have the malignant disease of interest, placing said pooled whole bloods in solution, concentrating putative antigenic malignant disease-sensitized components present in said pooled whole bloods, separating said concentrated components from said solution, and clarifying said components to form said blood extract.
Claim 41
Independent Claim
92 words
A method for the preparation of a putative antigenic blood extract specific to a particular malignant disease of interest which comprises the steps of pooling the whole bloods from a plurality of donors known to have the malignant disease of interest, placing said pooled whole bloods in non-isotonic solution, concentrating the putative antigenic malignant disease-sensitized components present in said pooled whole bloods, separating said concentrated components from said solution, placing the thusly separated and concentrated putative antigenic malignant disease-sensitized components in isotonic solution, and clarifying said components to form said blood extract.
Claim 42
Independent Claim
92 words
A method for the preparation of a putative antigenic blood extract specific to a particular malignant disease of interest which comprises the steps of pooling the whole bloods from a plurality of donors known to have the malignant disease of interest, placing said pooled whole bloods in non-isotonic solution, concentrating the putative antigenic malignant disease-sensitized components present in said pooled whole bloods, separating said concentrated components from said solution, placing the thusly separated and concentrated putative antigenic malignant disease-sensitized components in isotonic solution, and clarifying said components to form said blood extract.
Claim 43
Independent Claim
68 words
A method for the diagnosis of multiple sclerosis by blood sample analysis which comprises the steps of mixing a putative antigenic blood extract prepared from multiple sclerosis-sensitized blood and specific to multiple sclerosis-sensitized blood leukocytes with leukocytes of said blood sample and promoting the reaction therebetween to detect the presence of multiple sclerosis-sensitized leukocytes in the blood sample, and quantifying the amount of multiple sclerosis disease-sensitized blood leukocytes.
Claim 44
Independent Claim
68 words
A method for the diagnosis of multiple sclerosis by blood sample analysis which comprises the steps of mixing a putative antigenic blood extract prepared from multiple sclerosis-sensitized blood and specific to multiple sclerosis-sensitized blood leukocytes with leukocytes of said blood sample and promoting the reaction therebetween to detect the presence of multiple sclerosis-sensitized leukocytes in the blood sample, and quantifying the amount of multiple sclerosis disease-sensitized blood leukocytes.
Claim 45
Dependent Claim
39 words
The method of claim 31 further comprising the step of pooling the whole bloods from a plurality of donors known to have multiple sclerosis and preparing said blood extract therefrom for mixing with the leukocytes of said blood sample.
Claim 46
Independent Claim
76 words
A method for the diagnosis of a specific malignant disease of interest by blood sample analysis which comprises the steps of mixing a putative antigenic blood extract prepared from blood sensitized to said malignant disease, said extract being specific to malignant disease-sensitized blood leukocytes, with leukocytes of said blood sample and promoting the reaction therebetween to detect the presence of malignant disease-sensitized leukocytes in the blood sample, and quantifying the amount of malignant disease-sensitized blood leukocytes.
Claim 47
Independent Claim
76 words
A method for the diagnosis of a specific malignant disease of interest by blood sample analysis which comprises the steps of mixing a putative antigenic blood extract prepared from blood sensitized to said malignant disease, said extract being specific to malignant disease-sensitized blood leukocytes, with leukocytes of said blood sample and promoting the reaction therebetween to detect the presence of malignant disease-sensitized leukocytes in the blood sample, and quantifying the amount of malignant disease-sensitized blood leukocytes.
Claim 48
Dependent Claim
43 words
The method of claim 33 further comprising the step of pooling the whole bloods from a plurality of donors known to have the specific malignant disease of interest and preparing said blood extract therefrom for mixing with the leukocytes of said blood sample.
Claim 49
Independent Claim
68 words
A method for the diagnosis of multiple sclerosis by blood sample analysis which comprises the steps of mixing a putative antigenic urine extract prepared from multiple sclerosis-sensitized urine and specific to multiple sclerosis-sensitized blood leukocytes with leukocytes of said blood sample and promoting the reaction therebetween to detect the presence of multiple sclerosis-sensitized leukocytes in the blood sample, and quantifying the amount of multiple sclerosis disease-sensitized blood leukocytes.
Claim 50
Independent Claim
68 words
A method for the diagnosis of multiple sclerosis by blood sample analysis which comprises the steps of mixing a putative antigenic urine extract prepared from multiple sclerosis-sensitized urine and specific to multiple sclerosis-sensitized blood leukocytes with leukocytes of said blood sample and promoting the reaction therebetween to detect the presence of multiple sclerosis-sensitized leukocytes in the blood sample, and quantifying the amount of multiple sclerosis disease-sensitized blood leukocytes.
Claim 51
Dependent Claim
38 words
A method of claim 35 further comprising the step of pooling the urine from a plurality of donors known to have multiple sclerosis and preparing said urine extract therefrom for mixing with the leukocytes of said blood sample.
Claim 52
Independent Claim
79 words
A method for the diagnosis of a specific malignant disease of interest by blood sample analysis which comprises the steps of mixing a putative antigenic body substance extract prepared from body substance sensitized to said from malignant disease, said extract being specific to malignant disease-sensitized blood leukocytes, with leukocytes of said blood sample and promoting the reaction therebetween to detect the presence of malignant disease-sensitized leukocytes in the blood sample, and quantifying the amount of malignant disease-sensitized blood leukocytes.
Claim 53
Independent Claim
79 words
A method for the diagnosis of a specific malignant disease of interest by blood sample analysis which comprises the steps of mixing a putative antigenic body substance extract prepared from body substance sensitized to said from malignant disease, said extract being specific to malignant disease-sensitized blood leukocytes, with leukocytes of said blood sample and promoting the reaction therebetween to detect the presence of malignant disease-sensitized leukocytes in the blood sample, and quantifying the amount of malignant disease-sensitized blood leukocytes.
Claim 54
Dependent Claim
43 words
The method of claim 37 further comprising the step of pooling the body substance of a plurality of donors known to have the malignant disease of interest and preparing said body substance extract therefrom for mixing with the leukocytes of said blood sample.
Claim 55
Dependent Claim
42 words
The method of claim 37 further comprising the step of pooling the pleural fluids of a plurality of donors known to have the malignant disease of interest and preparing said pleural extract therefrom for mixing with the leukocytes of said blood sample.
Claim 56
Dependent Claim
42 words
The method of claim 37 further comprising the step of pooling the ascites fluids of a plurality of donors known to have the malignant disease of interest and preparing said ascite extract therefrom for mixing with the leukocytes of said blood sample.
Claim 57
Dependent Claim
47 words
The method of claim 37 further comprising the step of pooling the supernates of tumor cell cultures of a plurality of donors known to have the specific malignant disease of interest and extracting said tumor cell extract therefrom for mixing with the leukocytes of said blood sample.
Claim 58
Dependent Claim
47 words
The method of claim 37 further comprising the step of pooling tumor cells grown in culture media from a plurality of donors known to have the specific malignant disease of interest and preparing said tumor cell extract therefrom for mixing with the leukocytes of said blood sample.
Claim 59
Independent Claim
66 words
A method for the preparation of a putative antigenic urine extract specific to multiple sclerosis which comprises the steps of pooling the urines of a plurality of donors known to have multiple sclerosis, placing said pooled urines in solution, concentrating putative antigenic multiple sclerosis-sensitized components present in said pooled urines, separating said concentrated components from said solution, and clarifying said components to form said urine extract.
Claim 60
Independent Claim
66 words
A method for the preparation of a putative antigenic urine extract specific to multiple sclerosis which comprises the steps of pooling the urines of a plurality of donors known to have multiple sclerosis, placing said pooled urines in solution, concentrating putative antigenic multiple sclerosis-sensitized components present in said pooled urines, separating said concentrated components from said solution, and clarifying said components to form said urine extract.
Claim 61
Dependent Claim
17 words
The method of claim 43 wherein said pooled urines in solution are mixed with a non-isotonic medium.
Claim 62
Independent Claim
73 words
A method for the preparation of a putative antigenic urine extract specific to a particular malignant disease of interest which comprises the steps of pooling the urines from a plurality of donors known to have the malignant disease of interest, placing said pooled urines in solution, concentrating putative antigenic malignant disease-sensitized components present in said pooled urine, separating said concentrated components from said solution, and clarifying said components to form said urine extract.
Claim 63
Independent Claim
73 words
A method for the preparation of a putative antigenic urine extract specific to a particular malignant disease of interest which comprises the steps of pooling the urines from a plurality of donors known to have the malignant disease of interest, placing said pooled urines in solution, concentrating putative antigenic malignant disease-sensitized components present in said pooled urine, separating said concentrated components from said solution, and clarifying said components to form said urine extract.
Claim 64
Dependent Claim
17 words
The method of claim 45 wherein said pooled urines in solution are mixed with a non-isotonic medium.
Claim 65
Independent Claim
75 words
A method for the preparation of a putative antigenic pleural extract specific to a malignant disease of interest which comprises the steps of pooling the pleural fluids of a plurality of donors known to have the malignant disease of interest, placing said pooled pleural fluids in solution, concentrating putative antigenic malignant disease-sensitized components present in said pooled pleural fluids, separating said concentrated components from said solution, and clarifying said components to form said pleural extract.
Claim 66
Independent Claim
75 words
A method for the preparation of a putative antigenic pleural extract specific to a malignant disease of interest which comprises the steps of pooling the pleural fluids of a plurality of donors known to have the malignant disease of interest, placing said pooled pleural fluids in solution, concentrating putative antigenic malignant disease-sensitized components present in said pooled pleural fluids, separating said concentrated components from said solution, and clarifying said components to form said pleural extract.
Claim 67
Independent Claim
74 words
A method for the preparation of a putative antigenic ascite extract specific to a malignant disease of interest which comprises the steps of pooling the ascite extract of a plurality of donors known to have the malignant disease of interest, placing said pooled ascite extract in solution, concentrating putative antigenic malignant disease-sensitized components present in said pooled ascites, separating said concentrated components from said solution, and clarifying said components to form said ascite extract.
Claim 68
Independent Claim
74 words
A method for the preparation of a putative antigenic ascite extract specific to a malignant disease of interest which comprises the steps of pooling the ascite extract of a plurality of donors known to have the malignant disease of interest, placing said pooled ascite extract in solution, concentrating putative antigenic malignant disease-sensitized components present in said pooled ascites, separating said concentrated components from said solution, and clarifying said components to form said ascite extract.
Claim 69
Independent Claim
82 words
A method for the preparation of a putative antigenic tumor cell extract specific to a malignant disease of interest which comprises the steps of pooling the supernates of tumor cell cultures of a plurality of donors known to have the malignant disease of interest, placing said pooled supernates of tumor cell cultures in solution, concentrating putative antigenic malignant disease-sensitized components present in said pooled supernates, separating said concentrated components from said solution, and clarifying said components to form said tumor cell extract.
Claim 70
Independent Claim
82 words
A method for the preparation of a putative antigenic tumor cell extract specific to a malignant disease of interest which comprises the steps of pooling the supernates of tumor cell cultures of a plurality of donors known to have the malignant disease of interest, placing said pooled supernates of tumor cell cultures in solution, concentrating putative antigenic malignant disease-sensitized components present in said pooled supernates, separating said concentrated components from said solution, and clarifying said components to form said tumor cell extract.
Claim 71
Independent Claim
84 words
A method for the preparation of a putative antigenic tumor cell extract specific to a malignant disease of interest which comprises the steps of pooling tumor cell grown in culture media from a plurality of donors known to have the malignant disease of interest, placing said pooled tumor cells grown in culture media in solution, concentrating putative antigenic malignant disease-sensitized components present in said pooled tumor cells, separating said concentrated components from said solution, and clarifying said components to form said tumor cell extract.
Claim 72
Independent Claim
84 words
A method for the preparation of a putative antigenic tumor cell extract specific to a malignant disease of interest which comprises the steps of pooling tumor cell grown in culture media from a plurality of donors known to have the malignant disease of interest, placing said pooled tumor cells grown in culture media in solution, concentrating putative antigenic malignant disease-sensitized components present in said pooled tumor cells, separating said concentrated components from said solution, and clarifying said components to form said tumor cell extract.
References Cited (Backward Citations)
US4146603A
Cited
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