US4310504A
Expired / Lapsed
US
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Dry radioimmunoassay test composition containing stabilized label/antibody mixture
Assignee: Ames Yissum Ltd
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Filing: 1979-09-12
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Granted: 1982-01-12
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Expires: 1999-09-12
Abstract
Abstract A dry radioimmunoassay test composition comprising a mixture of a radiolabelled form of a ligand, i.e., a hapten or an antigen, an antibody for the ligand, and a heavy metal cation, e.g., cupric ion, to inhibit the complex-forming reaction between the radiolabelled ligand and the antibody. A method for preparing the dry test composition is also provided. In use in a radioimmunoassay, the dry test composition is reconstituted with an aqueous liquid and combined with a test sample and a chelating agent to bind the heavy metal cation, permitting reaction among any ligand present in the sample, the radiolabelled ligand, and the antibody. A test kit for performing such radioimmunoassay is also provided.
Inventors
- 👤 Sara Derfler
- 👤 Yehuday Tamir
- 👤 Daniel B. Wagner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 22:17
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/826
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
95 words
A method for preparing a dry radioimmunoassay test composition comprising a mixture of (i) a radiolabelled form of a ligand selected from haptens and antigens and (ii) an antibody for said ligand, wherein substantially all of said radiolabelled ligand is not bound to said antibody in said mixture, which method comprises the steps of: (a) preparing an aqueous liquid mixture of said radiolabelled ligand, said antibody, and a heavy metal cation present in an amount sufficient to inhibit substantially the complex-forming reaction between said radiolabelled ligand and said antibody, and (b) drying said liquid mixture.
Claim 2
Independent Claim
95 words
A method for preparing a dry radioimmunoassay test composition comprising a mixture of (i) a radiolabelled form of a ligand selected from haptens and antigens and (ii) an antibody for said ligand, wherein substantially all of said radiolabelled ligand is not bound to said antibody in said mixture, which method comprises the steps of: (a) preparing an aqueous liquid mixture of said radiolabelled ligand, said antibody, and a heavy metal cation present in an amount sufficient to inhibit substantially the complex-forming reaction between said radiolabelled ligand and said antibody, and (b) drying said liquid mixture.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein said heavy metal cation is cupric ion.
Claim 4
Dependent Claim
18 words
The method of claim 1 or 2 wherein said drying step is accomplished by lyophilizing said liquid mixture.
Claim 5
Dependent Claim
32 words
The method of claim 1 wherein step (a) is accomplished by preparing an aqueous solution containing said heavy metal cation and said antibody and thereafter adding to said solution said radiolabelled ligand.
Claim 6
Dependent Claim
10 words
The method of claim 1 wherein said ligand is thyroxine.
Claim 7
Dependent Claim
50 words
A dry radioimmunoassay test composition comprising a mixture of (i) a radiolabelled form of a ligand selected from haptens and antigens, (ii) an antibody for said ligand, and (iii) a heavy metal cation in an amount sufficient to inhibit substantially the complex-forming reaction between said radiolabelled ligand and said antibody.
Claim 8
Dependent Claim
50 words
A dry radioimmunoassay test composition comprising a mixture of (i) a radiolabelled form of a ligand selected from haptens and antigens, (ii) an antibody for said ligand, and (iii) a heavy metal cation in an amount sufficient to inhibit substantially the complex-forming reaction between said radiolabelled ligand and said antibody.
Claim 9
Dependent Claim
13 words
The composition of claim 6 wherein said heavy metal cation is cupric ion.
Claim 10
Dependent Claim
12 words
The composition of claim 6 or 7 wherein said ligand is thyroxine.
Claim 11
Dependent Claim
171 words
A radioimmunoassay method for the determination of a ligand selected from haptens and antigens in a liquid sample, comprising the steps of: (a) providing a dry test composition comprising a mixture of (i) a radiolabelled form of said ligand, (ii) an antibody for said ligand, and (iii) a heavy metal cation in an amount sufficient to inhibit substantially the complex-forming reaction between said radiolabelled ligand and said antibody, (b) reconstituting said composition with a predetermined volume of an aqueous liquid, (c) combining the reconstituted liquid composition with said liquid sample and with a chelating agent for said heavy metal cation in an amount sufficient to bind substantially all of said cation present, (d) incubating the resulting liquid reaction mixture, (e) separating the fraction of said radiolabelled ligand which becomes bound to said antibody during the incubation period from that which does not become so bound, and (f) measuring the radioactivity of one of the separated fractions as a function of the presence or amount of said ligand in said liquid sample.
Claim 12
Dependent Claim
171 words
A radioimmunoassay method for the determination of a ligand selected from haptens and antigens in a liquid sample, comprising the steps of: (a) providing a dry test composition comprising a mixture of (i) a radiolabelled form of said ligand, (ii) an antibody for said ligand, and (iii) a heavy metal cation in an amount sufficient to inhibit substantially the complex-forming reaction between said radiolabelled ligand and said antibody, (b) reconstituting said composition with a predetermined volume of an aqueous liquid, (c) combining the reconstituted liquid composition with said liquid sample and with a chelating agent for said heavy metal cation in an amount sufficient to bind substantially all of said cation present, (d) incubating the resulting liquid reaction mixture, (e) separating the fraction of said radiolabelled ligand which becomes bound to said antibody during the incubation period from that which does not become so bound, and (f) measuring the radioactivity of one of the separated fractions as a function of the presence or amount of said ligand in said liquid sample.
Claim 13
Dependent Claim
13 words
The method of claim 9 wherein said heavy metal cation is cupric ion.
Claim 14
Dependent Claim
13 words
The method of claim 10 wherein said chelating agent is ethylenediamine tetraacetic acid.
Claim 15
Dependent Claim
26 words
The method of claim 9 wherein in step (c) said reconstituted liquid composition is combined first with said chelating agent and thereafter with said liquid sample.
Claim 16
Dependent Claim
24 words
The method of claim 9 wherein in step (c) said reconstituted liquid composition is combined simultaneously with said liquid sample and said chelating agent.
Claim 17
Independent Claim
12 words
The method of any of claims 9-13 wherein said ligand is thyroxine.
Claim 18
Dependent Claim
85 words
A test kit for the radioimmunoassay determination of a ligand selected from haptens and antigens in a liquid sample, comprising, in a packaged combination, (a) a container holding a dry test composition comprising a mixture of (i) a radiolabelled form of said ligand, (ii) an antibody for said ligand, and (iii) a heavy metal cation in an amount sufficient to inhibit substantially the complex-forming reaction between said radiolabelled ligand and said antibody, and (b) a container holding a chelating agent for said heavy metal cation.
Claim 19
Dependent Claim
85 words
A test kit for the radioimmunoassay determination of a ligand selected from haptens and antigens in a liquid sample, comprising, in a packaged combination, (a) a container holding a dry test composition comprising a mixture of (i) a radiolabelled form of said ligand, (ii) an antibody for said ligand, and (iii) a heavy metal cation in an amount sufficient to inhibit substantially the complex-forming reaction between said radiolabelled ligand and said antibody, and (b) a container holding a chelating agent for said heavy metal cation.
Claim 20
Dependent Claim
13 words
The kit of claim 15 wherein said heavy metal cation is cupric ion.
Claim 21
Dependent Claim
13 words
The kit of claim 16 wherein said chelating agent is ethylenediamine tetraacetic acid.
Claim 22
Dependent Claim
15 words
The kit of claim 15 wherein said chelating agent is dissolved in an aqueous liquid.
Claim 23
Dependent Claim
12 words
The kit of any of claims 15-18 wherein said ligand is thyroxine.
References Cited (Backward Citations)
US4017597A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-01-11 | 1985-07-13 | 1986-01-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-01-11 | 1989-07-13 | 1990-01-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-01-11 | 1993-07-13 | 1994-01-12 | $7700.00 | Window Closed / Lapsed |