US4307193A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of producing interferon
Assignee: Toray Industries Inc
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Filing: 1979-11-20
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Granted: 1981-12-22
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Expires: 1999-11-20
Abstract
Abstract In the method of the present invention for production of interferon from propagating tissue culture cells, a propagating apparatus is utilized having a plurality of spaced-apart parallel flat plates. Monolayers of the tissue cells are grown on these plates and the apparatus is adapted for the introduction of gas and/or liquid mediums between the plates. In carrying out the method of the present invention the culture medium which nourishes the propagating cell tissues is exposed to a gas phase for oxygenating and adjusting the pH of the medium, while maintaining the culture medium and the propagated cells in a static and undisturbed condition.
Inventors
- 👤 Masahiko Iizuka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 12:40
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C12; C12M; C12M23/00; C12M23/34; C12M23/50; C12M25/00; C12M25/06; Y; Y10; Y10S; Y10S435/00; Y10S435/811
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
109 words
A method of producing interferon, comprising the steps of: providing a plurality of substantially flat, substantially parallel plates adapted for the cultivation of tissue cells; sterilizing said plates; introducing a cell suspension onto said plates wherein said suspension comprises tissue cells and a culture medium; forming confluent monolayers of cells on said plates; oxygenating and adjusting the pH of said culture medium by exposure to a gas phase while maintaining said medium and said tissue cell monolayers in a static and undisturbed condition; exposing said tissue cell monolayers to interferon inducers and enhancers incubating said exposed tissue cell monolayers for formation of interferon therefrom; and collecting the crude interferon.
Claim 2
Independent Claim
109 words
A method of producing interferon, comprising the steps of: providing a plurality of substantially flat, substantially parallel plates adapted for the cultivation of tissue cells; sterilizing said plates; introducing a cell suspension onto said plates wherein said suspension comprises tissue cells and a culture medium; forming confluent monolayers of cells on said plates; oxygenating and adjusting the pH of said culture medium by exposure to a gas phase while maintaining said medium and said tissue cell monolayers in a static and undisturbed condition; exposing said tissue cell monolayers to interferon inducers and enhancers incubating said exposed tissue cell monolayers for formation of interferon therefrom; and collecting the crude interferon.
Claim 3
Dependent Claim
183 words
In a method of producing interferon, the steps comprising: providing an apparatus for propagating tissue culture cells comprising a plurality of spaced-apart, substantially flat plates on which cells, liquid and gas phases can exist, and means for maintaining said plates substantially parallel to each other, in which said plates are enclosed by frames to form dishes, wherein said dishes are easily separable from each other and are easily separable from the other components of the apparatus; sterilizing said apparatus; introducing a cell suspension to said plate, said suspension comprising tissue cells and a culture medium; forming substantially confluent monolayers of said cells on said plates; oxygenating and adjusting the pH of said medium by exposure to a gas phase while maintaining said medium and said tissue cell monolayers in a static and undisturbed condition; discarding the culture medium; stimulating said cells to produce interferon by exposure to interferon inducer and by irradiation of said cell monolayer with UV light; refeeding said cell monolayers with fresh serum-free medium; incubating said cell monolayers until sufficient interferon accumulates in the medium; and harvesting the crude interferon.
Claim 4
Dependent Claim
183 words
In a method of producing interferon, the steps comprising: providing an apparatus for propagating tissue culture cells comprising a plurality of spaced-apart, substantially flat plates on which cells, liquid and gas phases can exist, and means for maintaining said plates substantially parallel to each other, in which said plates are enclosed by frames to form dishes, wherein said dishes are easily separable from each other and are easily separable from the other components of the apparatus; sterilizing said apparatus; introducing a cell suspension to said plate, said suspension comprising tissue cells and a culture medium; forming substantially confluent monolayers of said cells on said plates; oxygenating and adjusting the pH of said medium by exposure to a gas phase while maintaining said medium and said tissue cell monolayers in a static and undisturbed condition; discarding the culture medium; stimulating said cells to produce interferon by exposure to interferon inducer and by irradiation of said cell monolayer with UV light; refeeding said cell monolayers with fresh serum-free medium; incubating said cell monolayers until sufficient interferon accumulates in the medium; and harvesting the crude interferon.
Claim 5
Dependent Claim
15 words
The method defined in claim 4 wherein the interferon inducer is a polyriboinosinic-polyribocytidylic acid complex.
Claim 6
Dependent Claim
37 words
The method defined in claim 4 comprising the further steps of: removing each dish from said propagation apparatus for irradiation of said cell monolayers, and replacing said dishes so as to reform said apparatus under aseptic conditions.
Claim 7
Dependent Claim
17 words
The method defined in claim 3 wherein said interferon inducers and enhancers are cycloheximide and actenomycin D.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-12-21 | 1985-06-22 | 1985-12-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-12-21 | 1989-06-22 | 1989-12-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-12-21 | 1993-06-22 | 1993-12-22 | $7700.00 | Window Closed / Lapsed |