US4306022A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cocoa bean cell culture
Assignee: Cornell Research Foundation Inc
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Filing: 1980-03-11
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Granted: 1981-12-15
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Expires: 2000-03-11
Abstract
Abstract The invention relates to culturing cocoa bean cells in the presence of a cocoa bean cell culture growth supporting medium for a time and at a temperature sufficient to produce a metabolite, e.g. triglyceride species similar to those normally found in cocoa butter and isolating said metabolite.
Inventors
- 👤 John E. Kinsella
- 👤 Chee H. Tsai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 21:14
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/64; C12P7/6436; C12P7/6445; C12P7/6463; C12N; C12N5/00; C12N5/04; C12P7/6472
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
52 words
A method of producing SUS triglycerides which comprises cultivating cocoa bean cells by suspension cell culture in the presence of a cocoa bean cell growth supporting medium for a time and at a temperature sufficient to propagate said cells thereby forming SUS triglyceride metabolites and separating said triglycerides from the cell culture.
Claim 2
Independent Claim
52 words
A method of producing SUS triglycerides which comprises cultivating cocoa bean cells by suspension cell culture in the presence of a cocoa bean cell growth supporting medium for a time and at a temperature sufficient to propagate said cells thereby forming SUS triglyceride metabolites and separating said triglycerides from the cell culture.
Claim 3
Dependent Claim
24 words
A method as in claim 1 wherein the fatty acid contained in said triglycerides comprise palmitic acid, stearic acid, oleic acid and linoleic acid.
Claim 4
Independent Claim
37 words
A method as in claims 1 or 2 wherein cocoa bean cotyledon is first grown on a solid or semisolid medium to form callus and then the callus resultant is subsequently subjected to said suspension cell culture.
Claim 5
Dependent Claim
15 words
A method as in claim 3 wherein the solid or semisolid medium is agar based.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-12-14 | 1985-06-15 | 1985-12-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-12-14 | 1989-06-15 | 1989-12-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-12-14 | 1993-06-15 | 1993-12-15 | $7700.00 | Window Closed / Lapsed |