US4303680A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of yeast extract containing flavoring
Assignee: Ajinomoto Co Inc
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Filing: 1980-01-02
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Granted: 1981-12-01
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Expires: 2000-01-02
Abstract
Abstract A yeast extract containing flavoring 5'-neucleotide and having an improved thickness or body in taste is produced by (1) autolyzing suspended yeast cells in the presence of a stimulator of autolysis at a constant pH ranging from 6.0 to 6.6, then (2) heating the autolyzed suspension at a temperature of 90°-110° C. for 1 to 3 hours thereby extracting intracellular ribonucleic acid; and thereafter performing the following steps in any order; (3) hydrolyzing the extracted ribonucleic acid with a 5'-phosphodiesterase and; (4) separating the resulting extract from the insoluble residue.
Inventors
- 👤 Tetsuo Tanekawa
- 👤 Hiroshi Takashima
- 👤 Tomoyoshi Hachiya
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 09:52
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/06; C12N1/063; A; A23; A23L; A23L33/00; A23L33/10; A23L33/14; A23L33/145
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
21 words
A method for producing a yeast extract containing flavoring 5'-nucleotide and having an improved thickness or body in taste which comprises:
Claim 2
Independent Claim
21 words
A method for producing a yeast extract containing flavoring 5'-nucleotide and having an improved thickness or body in taste which comprises:
Claim 3
Dependent Claim
100 words
(1) autolysing suspended yeast cells in the absence of added enzymes and added sodium chloride, and in the presence of an organic material that stimulates autolysis at a constant pH ranging from 6.0 to 6.6, then (2) heating the autolysed suspension at a temperature of 90° to 100° C. for 1 to 3 hours in the absence of added sodium chloride thereby extracting intracellular ribonucleic acid; and thereafter performing the following steps in any order; (3) hydrolysing the extracted ribonucleic acid with a 5'-phosphodiesterase to form nucleotides including adenosine monophosphate and; (4) separating the resulting extract from the insoluble residue.
Claim 4
Dependent Claim
100 words
(1) autolysing suspended yeast cells in the absence of added enzymes and added sodium chloride, and in the presence of an organic material that stimulates autolysis at a constant pH ranging from 6.0 to 6.6, then (2) heating the autolysed suspension at a temperature of 90° to 100° C. for 1 to 3 hours in the absence of added sodium chloride thereby extracting intracellular ribonucleic acid; and thereafter performing the following steps in any order; (3) hydrolysing the extracted ribonucleic acid with a 5'-phosphodiesterase to form nucleotides including adenosine monophosphate and; (4) separating the resulting extract from the insoluble residue.
Claim 5
Dependent Claim
18 words
The method of claim 1 wherein step (2) is followed by steps (3) and (4) in consecutive order.
Claim 6
Dependent Claim
18 words
The method of claim 1 wherein step (2) is followed by steps (4) and (3) in consecutive order.
Claim 7
Dependent Claim
16 words
The method of claim 1 wherein said 5'-phosphodiesterase is that derived from malts or malt roots.
Claim 8
Dependent Claim
23 words
The method of claim 1 wherein said step (3) further comprises converting adenosine monophosphate to inosine monophosphate by means of a deaminase enzyme.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-30 | 1985-06-01 | 1985-12-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-30 | 1989-06-01 | 1989-12-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-30 | 1993-06-01 | 1993-12-01 | $7700.00 | Window Closed / Lapsed |