US4297273A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Chemiluminescent phthalhydrazide-labeled protein and polypeptide conjugates
Assignee: Bayer Corp
•
Filing: 1980-01-14
•
Granted: 1981-10-27
•
Expires: 2000-01-14
Abstract
Abstract Chemiluminescent-labeled conjugates of the formula: <IMAGE> wherein one of R1 and R2 is hydrogen and the other is -NR3R4; R3 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and R4 is L(CO)-HN-(CH2)n wherein n=2-8 and L(CO)- is an antigenic protein or polypeptide bound through an amide bond. The labeled conjugates are useful as reagents in specific binding assays for determining antigenic proteins or polypeptides, or their binding partners, in liquid media.
Inventors
- 👤 Robert T. Buckler
- 👤 Hartmut R. Schroeder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 06:27
CPC Classifications
C; C07; C07D; C07D209/00; C07D209/02; C07D209/44; C07D209/48; C07D237/00; C07D237/26; C07D237/30; C07D237/32; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; Y; Y10; Y10S; Y10S435/00; Y10S435/968; Y10S436/00; Y10S436/80; Y10S436/805; Y10S530/00; Y10S530/802; Y10S530/806
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
60 words
In a chemiluminescent-labeled conjugate for use in specific binding assays of the general formula: L(CO--NH) labeling substance wherein L(CO)-- is an antigenic protein or polypeptide bound to said labeling substance through an amide bond, characterized in that said --NH) labeling substance has the formula: ##STR31## wherein R 3 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and n=2-8.
Claim 2
Dependent Claim
60 words
In a chemiluminescent-labeled conjugate for use in specific binding assays of the general formula: L(CO--NH) labeling substance wherein L(CO)-- is an antigenic protein or polypeptide bound to said labeling substance through an amide bond, characterized in that said --NH) labeling substance has the formula: ##STR31## wherein R 3 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and n=2-8.
Claim 3
Dependent Claim
20 words
The labeled conjugate of claim 1 wherein said antigenic protein or polypeptide has a molecular weight between 1,000 and 4,000,000.
Claim 4
Dependent Claim
15 words
The labeled conjugate of claim 1 wherein said antigenic protein or polypeptide is an antibody.
Claim 5
Dependent Claim
14 words
The labeled conjugate of claim 3 wherein said antibody is of the IgG class.
Claim 6
Dependent Claim
14 words
The labeled conjugate of claim 1 wherein said antigenic protein or polypeptide is insulin.
Claim 7
Dependent Claim
16 words
The labeled conjugate of claim 1 wherein said antigenic protein or polypeptide is thyroid stimulating hormone.
Claim 8
Dependent Claim
16 words
The labeled conjugate of claim 1 wherein said antigenic protein or polypeptide is a hepatitis-associated antigen.
Claim 9
Dependent Claim
33 words
The labeled conjugate of any one of claims 1-7 wherein said --NH) labeling substance has the formula: ##STR32## wherein R 3 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and n=2-8.
Claim 10
Dependent Claim
10 words
The labeled conjugate of claim 8 wherein n=4 or 6.
Claim 11
Dependent Claim
11 words
The labeled conjugate of claim 9 wherein R 3 is ethyl.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-10-26 | 1985-04-27 | 1985-10-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-26 | 1989-04-27 | 1989-10-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-10-26 | 1993-04-27 | 1993-10-27 | $7700.00 | Window Closed / Lapsed |