US4293643A
Expired / Lapsed
US
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Rotary shaking culture measuring method and apparatus
Assignee: Ohtake Works Co Ltd
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Filing: 1980-01-28
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Granted: 1981-10-06
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Expires: 2000-01-28
Abstract
Abstract Rotary shaking culture measuring method and apparatus characterizes in that culture tubes are disposed radially and at equal intervals in the circumferential direction, and an inclined turntable is disposed at a predetermined angle of inclination with respect to a horizontal reference plane, a central shaft of the inclined turntable serves as a driving shaft whereby a turning motion in a continuous or intermittently indexing form is imparted to the turntable. In addition, the culture tubes revolve along an inclined circular orbit described by such turning motion, and the substance being cultured within the tubes is shaked and cultured by a reciprocative motion of inertial resultant force of centrifugal force and gravity. Furthermore, in a certain position of the turntable the growth of the substance being cultured within all the culture tubes is measured with the lapse of time by a signal obtained at every fixed cycle.
Inventors
- 👤 Yukio Ohtake
- 👤 Masahiro Nakamura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 18:54
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/02; C12M23/08; B; B01; B01F; B01F29/00; B01F29/30; B01F29/32; B01F29/321; C12M23/50; C12M27/00; C12M27/10; C12M27/16; C12M41/00; C12M41/30; C12M41/36
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
225 words
A rotary shaking culture measuring apparatus comprising a rotatable turntable means rotatable about an axis which is disposed at an acute angle relative to vertical, said turntable means comprising a turntable element rotatable in a plane generally perpendicular to said axis, a plurality of radially spaced culture means carried on said turntable means, said culture means each being generally L-shaped and each having an upright portion joined to a lateral portion, said upright portion being disposed generally parallel to said axis and said lateral portion being generally parallel to said turntable element such that upon rotation of said turntable means, said culture means is stirred by the resultant force of gravity and centrifugal force, retaining means retaining said culture means on said turntable means, said turntable element having a plurality of concentration detection openings, a concentration measuring device disposed adjacent to said turntable means comprising a light source and a light detector device operable in association with said concentration detection openings to provide a concentration signal indicative of the concentration in said culture means, and a synchronizing device disposed adjacent to said turntable means operable to detect passage of said culture means past a predetermined position and thereby provide a synchronization signal for synchronizing the measuring of said concentration by said concentration measuring device as said culture means pass successively by said concentration measuring device.
Claim 2
Dependent Claim
225 words
A rotary shaking culture measuring apparatus comprising a rotatable turntable means rotatable about an axis which is disposed at an acute angle relative to vertical, said turntable means comprising a turntable element rotatable in a plane generally perpendicular to said axis, a plurality of radially spaced culture means carried on said turntable means, said culture means each being generally L-shaped and each having an upright portion joined to a lateral portion, said upright portion being disposed generally parallel to said axis and said lateral portion being generally parallel to said turntable element such that upon rotation of said turntable means, said culture means is stirred by the resultant force of gravity and centrifugal force, retaining means retaining said culture means on said turntable means, said turntable element having a plurality of concentration detection openings, a concentration measuring device disposed adjacent to said turntable means comprising a light source and a light detector device operable in association with said concentration detection openings to provide a concentration signal indicative of the concentration in said culture means, and a synchronizing device disposed adjacent to said turntable means operable to detect passage of said culture means past a predetermined position and thereby provide a synchronization signal for synchronizing the measuring of said concentration by said concentration measuring device as said culture means pass successively by said concentration measuring device.
Claim 3
Dependent Claim
50 words
A rotary shaking culture measuring apparatus according to claim 1 wherein said turntable element is a disk member disposed generally in a plane perpendicular to said axis of rotation, said retaining means comprising upright supports extending upright from said disk member for supporting said upright portions of said culture means.
Claim 4
Dependent Claim
35 words
A rotary shaking culture measuring apparatus according to claim 2 wherein said retaining means further comprises mounting members on said disk member for mounting said lateral portions of said culture means on said disk member.
Claim 5
Dependent Claim
63 words
A rotary shaking culture measuring apparatus according to claim 1 wherein said retaining means are constructed and located to mount said culture means at radial spaced positions on said disk element and with said lateral portions of said culture means disposed generally parallel to said disk element and with said upright portions of said culture means disposed generally perpendicular to said disk element.
Claim 6
Dependent Claim
93 words
A rotary shaking culture measuring apparatus according to claim 1 wherein said concentration detection openings underlie each of said lateral portions of said culture means, said light source being disposed on one side of said turntable element and said light detector device being disposed on the opposite side of said turntable element in optical alignment with said light source such that said lateral portions of said culture means and the underlying associated concentration detection openings pass successively between said optically aligned light source and light detector device as said turntable means is rotated.
Claim 7
Dependent Claim
87 words
A rotary shaking culture measuring apparatus according to claim 1 wherein said turntable element has a plurality of synchronization openings in radial alignment with said concentration detection openings, said synchronizing device comprising a light source on one side of said turntable element and a light detecting device on the opposite side of said turntable element which is in optical alignment with said light source such that said synchronization openings pass successively between said optically aligned light source and light detecting device as said turntable means is rotated.
Claim 8
Dependent Claim
23 words
A rotary shaking culture measuring apparatus according to claim 1 wherein said turntable means comprises drive means for continuously rotating said turntable element.
Claim 9
Dependent Claim
23 words
A rotary shaking culture measuring apparatus according to claim 1 wherein said turntable means comprises drive means for intermittently rotating said turntable element.
Claim 10
Dependent Claim
33 words
A rotary shaking culture measuring apparatus according to claim 1 further comprising recording means connected to said concentration measuring device and to said synchronizing device for recording said concentration of said culture means.
Claim 11
Dependent Claim
26 words
A rotary shaking culture measuring apparatus according to claim 1 further comprising means defining an enclosure about said turntable means providing a closed and controlled atmosphere.
Claim 12
Dependent Claim
197 words
A rotary shaking culture measuring method comprising revolving a plurality of culture means about a fixed axis disposed at an acute angle relative to vertical and along an inclined circular path which is disposed at a right angle relative to said axis, said culture means each being L-shaped having an upright portion opening at the top thereof and a lateral portion, said upright portion being generally parallel to said axis, stirring the substance being cultured in said culture means by said revolutionary motion utilizing the reciprocative motion of the inertial resultant force of centrifugal force and gravity, continuously measuring the degree of growth of the substance being cultured within each said culture means when said culture means passes a predetermined position on said inclined circular path, said continuous measurement being effected by obtaining a concentration signal and a synchronizing signal at every cycle of revolution by light sources each passing a light through said culture means and through said inclined circular path respectively, said synchronizing signal detecting when each of said culture means passes said predetermined position and thereby providing timing for measuring said concentration, and indicating the concentration of said substance in analog or digital forms.
Claim 13
Dependent Claim
197 words
A rotary shaking culture measuring method comprising revolving a plurality of culture means about a fixed axis disposed at an acute angle relative to vertical and along an inclined circular path which is disposed at a right angle relative to said axis, said culture means each being L-shaped having an upright portion opening at the top thereof and a lateral portion, said upright portion being generally parallel to said axis, stirring the substance being cultured in said culture means by said revolutionary motion utilizing the reciprocative motion of the inertial resultant force of centrifugal force and gravity, continuously measuring the degree of growth of the substance being cultured within each said culture means when said culture means passes a predetermined position on said inclined circular path, said continuous measurement being effected by obtaining a concentration signal and a synchronizing signal at every cycle of revolution by light sources each passing a light through said culture means and through said inclined circular path respectively, said synchronizing signal detecting when each of said culture means passes said predetermined position and thereby providing timing for measuring said concentration, and indicating the concentration of said substance in analog or digital forms.
Claim 14
Dependent Claim
31 words
A rotary shaking culture measuring method according to claim 11 in which said revolving of said culture means is effected by rotating an inclined turntable which carries thereon said culture means.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-10-05 | 1985-04-06 | 1985-10-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-05 | 1989-04-06 | 1989-10-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-10-05 | 1993-04-06 | 1993-10-06 | $7700.00 | Window Closed / Lapsed |