US4293537A
Expired / Lapsed
US
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Novel chemical method of labeling proteins with 99m Tc-Technetium at physiological condition
Assignee: Individual
•
Filing: 1978-09-05
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Granted: 1981-10-06
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Expires: 1998-09-05
Abstract
Abstract A novel rapid chemical method of labeling exogenous and autologous plasma proteins, other compound and/or substance containing proteins with 99m Tc-Technetium at physiologic pH 7.4 condition, producing a sterile apyrogenic radioactive tracer material which is suitable for biological and medical uses.
Inventors
- 👤 Dennis W. Wong
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 20:07
CPC Classifications
A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/08; A61K51/081; A61K51/088; A61K2123/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
123 words
A method of labeling human or animal plasma proteins, compounds or substances containing proteins with 99m Tc-pertechnetate at physiological pH 6-8 condition comprising the sequential steps of: a. treating 2-3 ml(60-100 mCi) 99m Tc-pertechnetate in normal saline with 0.5 ml of a solution of 0.1-5 mg stannous chloride or stannous tartrate in 0.05 N hydrochloric acid solution at room temperature for about 10 minutes; b. raising the pH of the acidic mixture of step (a) to 7.4 with a sufficient amount of pH 12.4 trisodium citrate/NaOH solution; c. adding from 0.1-50 mg of the desired protein to be labeled in less than 1 ml diluent to the admixture of step (b) and incubating at 37° C. or at room temperature for 30 minutes.
Claim 2
Independent Claim
123 words
A method of labeling human or animal plasma proteins, compounds or substances containing proteins with 99m Tc-pertechnetate at physiological pH 6-8 condition comprising the sequential steps of: a. treating 2-3 ml(60-100 mCi) 99m Tc-pertechnetate in normal saline with 0.5 ml of a solution of 0.1-5 mg stannous chloride or stannous tartrate in 0.05 N hydrochloric acid solution at room temperature for about 10 minutes; b. raising the pH of the acidic mixture of step (a) to 7.4 with a sufficient amount of pH 12.4 trisodium citrate/NaOH solution; c. adding from 0.1-50 mg of the desired protein to be labeled in less than 1 ml diluent to the admixture of step (b) and incubating at 37° C. or at room temperature for 30 minutes.
Claim 3
Dependent Claim
45 words
A method of detecting thromboembolism in patients by intravenous administration of 3-15 mCi 99m Tc-autologous human fibrinogen labeled according to the method of claim 1 and observing areas of increased radioactivity at the site of blood clots or active thrombosis as seen in scintigraphic scans.
Claim 4
Dependent Claim
15 words
Human serum albumin is labeled with 99m Tc according to the method of claim 1.
Claim 5
Dependent Claim
14 words
Human fibrinogen is labeled with 99m Tc according to the method of claim 1.
Claim 6
Dependent Claim
14 words
Human immunoglobulin is labeled with 99m Tc according to the method of claim 1.
Claim 7
Dependent Claim
16 words
A protein enzyme thrombin(bovine) is labeled with 99m Tc according to the method of claim 1.
Claim 8
Dependent Claim
16 words
A protein hormone thyrotropin(bovine) is labeled with 99m Tc according to the method of claim 1.
Claim 9
Dependent Claim
53 words
A method of detecting infectious foci in patients by intravenous administration of 3-15 mCi 99m Tc-autologous human immunoglobulin labeled according to the method of claim 1 which contains the specific antibody against the antigen or microorganisms and observing areas of increased radioactivity at the sites of the infection as seen in scintigraphic scans.
Claim 10
Dependent Claim
55 words
A method of detecting benign or malignant tumors in patients by intravenous administration of 3-15 mCi 99m Tc-autologous human immunoglobulin labeled according to the method of claim 1 which contains the specific antibody against the specific tumor and observing areas of increased radioactivity at the sites of tumors or metastasis as seen in scintigraphic scans.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-10-05 | 1985-04-06 | 1985-10-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-05 | 1989-04-06 | 1989-10-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-10-05 | 1993-04-06 | 1993-10-06 | $7700.00 | Window Closed / Lapsed |