US4293536A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Estrophilin detection
Assignee: University Patents Inc
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Filing: 1979-05-10
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Granted: 1981-10-06
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Expires: 1999-05-10
Abstract
Abstract Estrogen receptor protein, estrophilin, in a tissue sample is detected and quantified by radiochemical methods involving selective binding of the protein to controlled pore glass beads. Also disclosed are methods for selectively removing undesirable degradation products from radiochemically labelled non-polar steroid reagents, especially estradiol.
Inventors
- 👤 Elwood V. Jensen
- 👤 Verne D. Hospelhorn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 12:00
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/743; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/817; Y10S436/824
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
91 words
A method for determining the quantity of estrophilin in a tissue sample, said method comprising: (1) contacting said sample with a plurality of porous glass beads having a pore diameter of from about 400 to about 1200 A to selectively adhere said estrophilin to the pore surfaces of said beads; (2) contacting estrophilin in said sample with radioactively labelled estradiol to form a stable complex association of estrophilin bound to estradiol; and (3) selectively removing laballed estradiol from complex association with estrophilin and measuring the quantity of labelled estradiol so removed.
Claim 2
Independent Claim
91 words
A method for determining the quantity of estrophilin in a tissue sample, said method comprising: (1) contacting said sample with a plurality of porous glass beads having a pore diameter of from about 400 to about 1200 A to selectively adhere said estrophilin to the pore surfaces of said beads; (2) contacting estrophilin in said sample with radioactively labelled estradiol to form a stable complex association of estrophilin bound to estradiol; and (3) selectively removing laballed estradiol from complex association with estrophilin and measuring the quantity of labelled estradiol so removed.
Claim 3
Dependent Claim
30 words
The method of claim 1 wherein the step (1) of contacting said sample with said beads is carried out at a temperature of from about 1° to about 6° C.
Claim 4
Dependent Claim
35 words
The method of claim 1 wherein the step (2) of forming a complex association of labelled estradiol bound to estrophilin precedes the step (1) of selectively adhering estrophilin to pore surfaces of the glass beads.
Claim 5
Dependent Claim
35 words
The method of claim 1 wherein the step (2) of forming a complex association of labelled estradiol bound to estrophilin follows the step (1) of selectively adhering estrophilin to pore surfaces of said glass beads.
Claim 6
Dependent Claim
97 words
The method of claim 4 wherein the step (2) of forming a complex association of labelled estradiol bound to estrophilin comprises the following steps: (a) selectively dissociating non-labelled estradiol bound in complex association to estrophilin with a heavy metal ion by treatment with a water soluble source of heavy metal ions; (b) selectively displacing heavy metal ion bound in complex association with esrophilin by treatment with a water soluble sulfhydryl group-containing reagent capable of forming a soluble heavy metal salt with said metal ion; and, (c) contacting estrophilin adhered to said glass beads with radioactively labelled estrogen.
Claim 7
Dependent Claim
17 words
The method of claim 5 wherein said water soluble source of heavy metal ion is silver nitrate.
Claim 8
Dependent Claim
13 words
The method of claim 5 wherein said sulfhydryl group containing reagent is dithiothreitol.
Claim 9
Dependent Claim
19 words
The method of claim 5 wherein steps (b) and (c) are simultaneously practiced subsequent to practice of step (a).
Claim 10
Dependent Claim
17 words
The method of claim 1 wherein said glass beads have a pore diameter of about 500 A.
Claim 11
Dependent Claim
25 words
The method of claim 1 wherein said step (3) of removing labelled estradiol from complex association with estrophilin comprises the step of washing with ethanol.
Claim 12
Dependent Claim
14 words
The method of claim 1 wherein said radio-actively labelled estradiol is [6,7- 3 H]-estradiol-17β.
Claim 13
Dependent Claim
25 words
A plurality of porous glass beads having a pore diameter of from about 400 to 1200 A and having estrophilin adhered to pore surfaces thereof.
Claim 14
Dependent Claim
25 words
A plurality of porous glass beads having a pore diameter of from about 400 to 1200 A and having estrophilin adhered to pore surfaces thereof.
Claim 15
Independent Claim
50 words
A method for removing degradation products from a solution of radioactively labelled non-polar steroid, said method comprising contacting said solution with a plurality of porous glass beads having a pore diameter of from about 400 to 1200 A to selectively adhere said degradation products to pore surfaces of said beads.
Claim 16
Independent Claim
50 words
A method for removing degradation products from a solution of radioactively labelled non-polar steroid, said method comprising contacting said solution with a plurality of porous glass beads having a pore diameter of from about 400 to 1200 A to selectively adhere said degradation products to pore surfaces of said beads.
Claim 17
Dependent Claim
11 words
The process of claim 13 wherein said steroid is tritiated estradiol.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-10-05 | 1985-04-06 | 1985-10-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-05 | 1989-04-06 | 1989-10-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-10-05 | 1993-04-06 | 1993-10-06 | $7700.00 | Window Closed / Lapsed |