US4292331A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process and composition for the preservation of vegetables
Assignee: Ceva Sante Animale SA
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Filing: 1979-07-09
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Granted: 1981-09-29
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Expires: 1999-07-09
Abstract
Abstract A process for stabilizing and preserving harvested vegetables in an acidic environment within a silo. The acidity results from the significant degradation of starch, complex carbohydrates and fermentable carbohydrates. A mixture of bacteria capable of degrading the complex carbohydrates, bacteria capable of causing lactic fermentation of fermentable sugars, and enzymes having a complementary action capable of degrading the complex carbohydrates into fermentable sugars which may be used by the bacteria is used in the process. The bacteria composition may comprise a mixture of fungic amylases, amylases of bacterial origin, amyloglucosidase, and hemicellulase.
Inventors
- 👤 Louis Ostre
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 16:59
CPC Classifications
A; A23; A23K; A23K30/00; A23K30/10; A23K30/15; A23K30/18
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
84 words
In the process of ensilaging vegetables by lactic acidification comprising addition of sufficient bacteria causing lactic fermentation thereof to raise the pH to below about 4.5, the improvement which comprises additionally adding to said vegetables an agent which is capable of degrading higher carbohydrates into fermentable sugars usable by said lactic bacteria in an amount sufficient to produce sufficient fermentable sugars to realize said pH by said lactic fermentation, said agent being Enterobacteriacees bacteria capable of fermenting starch but not capable of fermenting maltose.
Claim 2
Dependent Claim
84 words
In the process of ensilaging vegetables by lactic acidification comprising addition of sufficient bacteria causing lactic fermentation thereof to raise the pH to below about 4.5, the improvement which comprises additionally adding to said vegetables an agent which is capable of degrading higher carbohydrates into fermentable sugars usable by said lactic bacteria in an amount sufficient to produce sufficient fermentable sugars to realize said pH by said lactic fermentation, said agent being Enterobacteriacees bacteria capable of fermenting starch but not capable of fermenting maltose.
Claim 3
Dependent Claim
36 words
Process of claim 1 wherein said agent is capable of fermenting glucose, Voges-Proskaver reagent and oxydase while having no effect on inositol sorbitol, citrate and urea and not resulting in the formation of H 2 S.
Claim 4
Dependent Claim
53 words
Process of claim 1 wherein said agent is disposed on a substrate; the amount of said agent culture and substrate in the dry state added per ton of vegetables to be ensilaged being between 10 and 15 kg., each gram of said culture-substrate mixture containing between 100,000 and 1,000,000 living germs per gram.
Claim 5
Dependent Claim
18 words
Process of claim 1 wherein said agent is genera of Erwinia or Pectobacterium, group Herbicola, named Enterobacter agglomerans.
Claim 6
Dependent Claim
17 words
Process of claim 1 wherein said agent is obtained by culturing in a nutritive medium of cereals.
Claim 7
Dependent Claim
36 words
Process of claim 1 wherein said agent is employed in combination with at least one enzyme having a complimentary action with said agent in a amount sufficient to accelerate the degradation of carbohydrates by said agent.
Claim 8
Dependent Claim
13 words
Process of claim 6 wherein said enzyme is disposed on a cereal substrate.
Claim 9
Dependent Claim
26 words
Process of claim 7 wherein the enzyme is disposed on the cereal in a ratio of approximately 1 kg. of enzyme per 9 kg. of substrate.
Claim 10
Dependent Claim
22 words
Process of claim 6, wherein the enzyme is chosen from the group consisting of fungic amylase, bacerial hemicellulase, amyloglucosidase and mixtures thereof.
Claim 11
Dependent Claim
31 words
Process of claim 9 wherein the enzyme is hemicellulase and is added in amounts of between 0.05 to 0.20 per thousand by weight and has an activity of approximately 35,000 U.I./g.
Claim 12
Dependent Claim
31 words
Process of claim 9 wherein the enzyme is amyloglucosidase and is added in an amount of 0.10 to 0.70 per thousand by weight and has an activity of 200 units AG/gram.
Claim 13
Dependent Claim
13 words
Process of claim 9 wherein said enzyme is disposed on a cereal substrate.
Claim 14
Dependent Claim
36 words
Process of claim 6 wherein the enzyme is bacterial amylase and is added in amounts of 0.05 to 0.20 per thousand by weight of the material to be ensilaged and has an activity of 250 units/gram.
Claim 15
Dependent Claim
38 words
Process of claim 6 wherein the enzyme is fungic amylase and is added in amounts of 0.10 to 0.20 per thousand by weight of the material to be ensilaged and has an activity of 50,000 units PS 50/gram.
Claim 16
Dependent Claim
27 words
Process of claim 7 wherein the bacteria causing the lactic fermentation are added in combination with and at the same time with said agent and said enzymes.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-09-28 | 1985-03-30 | 1985-09-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-09-28 | 1989-03-30 | 1989-09-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-09-28 | 1993-03-30 | 1993-09-29 | $7700.00 | Window Closed / Lapsed |