US4288557A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Antigenic complex from N. gonorrhoeae
Assignee: Merck and Co Inc
•
Filing: 1980-03-05
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Granted: 1981-09-08
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Expires: 2000-03-05
Abstract
Abstract An antigenic, immunogenic, non-toxic complex is obtained from the cell surface of N. gonorrhoeae.
Inventors
- 👤 Yashwant D. Karkhanis
- 👤 Dennis J. Carlo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 08:39
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/02; A61K39/095
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
134 words
A method of obtaining an antigenic, immunogenic, complex obtained from the cell surface of N. gonorrhoeae which is protective against N. gonorrhoeae having a molecular weight of from about 9,200,000 to about 9,500,000, over 80% of the complex being formed of five sub-units in approximately equal amount having molecular weight of about 68,000, about 50,000, about 34,000, about 27,000 and about 10,500, the complex containing a dry weight basis from about 90 to about 95% protein, up to about 7% lipopolysaccharide, about 2% carbohydrate and less than 1% of RNA, DNA and phospholipids which comprises stirring a homogenized cell paste of N. gonorrhoeae for from about 10 to about 15 hours, removing cellular debris, RNA and DNA from the homogenized extract, and separating a fraction having a molecular weight in excess of about 9,000,000.
Claim 2
Independent Claim
134 words
A method of obtaining an antigenic, immunogenic, complex obtained from the cell surface of N. gonorrhoeae which is protective against N. gonorrhoeae having a molecular weight of from about 9,200,000 to about 9,500,000, over 80% of the complex being formed of five sub-units in approximately equal amount having molecular weight of about 68,000, about 50,000, about 34,000, about 27,000 and about 10,500, the complex containing a dry weight basis from about 90 to about 95% protein, up to about 7% lipopolysaccharide, about 2% carbohydrate and less than 1% of RNA, DNA and phospholipids which comprises stirring a homogenized cell paste of N. gonorrhoeae for from about 10 to about 15 hours, removing cellular debris, RNA and DNA from the homogenized extract, and separating a fraction having a molecular weight in excess of about 9,000,000.
Claim 3
Dependent Claim
14 words
A method according to claim 1 wherein the cellular debris is removed by centrifugation.
Claim 4
Dependent Claim
21 words
A method according to claim 2 wherein the centrifugation is carried out at from about 30,000 xg to about 50,000 xg.
Claim 5
Dependent Claim
18 words
A method according to claim 2 wherein the RNA and DNA are removed by treatment with an enzyme.
Claim 6
Dependent Claim
14 words
A method according to claim 2 wherein the fraction is separated by gel filtration.
References Cited (Backward Citations)
US4220638A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-09-07 | 1985-03-09 | 1985-09-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-09-07 | 1989-03-09 | 1989-09-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-09-07 | 1993-03-09 | 1993-09-08 | $7700.00 | Window Closed / Lapsed |