US4288552A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilized intracellular enzymes
Assignee: Novo Nordisk AS
•
Filing: 1979-04-11
•
Granted: 1981-09-08
•
Expires: 1999-04-11
Abstract
Abstract Intracellular glutaraldehyde sensitive enzymes are immobilized by reacting microbial cell material with glutaraldehyde in the presence of a polyamine which is preferably a branched polyethylene imine.
Inventors
- 👤 Stina M. Gestrelius
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 16:36
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/04; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/911
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
88 words
Process for immobilization of an intracellular, glutaraldehyde sensitive enzyme, which process comprises reacting in an aqueous medium microbial cell material containing therein an intracellular glutaraldehyde sensitive enzyme with glutartaldehyde in the presence of a branched polyalkalene imine added to the medium before or simultaneously with the glutaraldehyde, said polyalkalene imine having primary amino groups on the polyalkalene imine molecule at a ratio of primary amine equivalents to aldehyde equivalents in the range of from 0.01:1 to 10:1, and thereafter recovering the resulting immobilized enzyme containing microbial cell preparation.
Claim 2
Dependent Claim
88 words
Process for immobilization of an intracellular, glutaraldehyde sensitive enzyme, which process comprises reacting in an aqueous medium microbial cell material containing therein an intracellular glutaraldehyde sensitive enzyme with glutartaldehyde in the presence of a branched polyalkalene imine added to the medium before or simultaneously with the glutaraldehyde, said polyalkalene imine having primary amino groups on the polyalkalene imine molecule at a ratio of primary amine equivalents to aldehyde equivalents in the range of from 0.01:1 to 10:1, and thereafter recovering the resulting immobilized enzyme containing microbial cell preparation.
Claim 3
Dependent Claim
14 words
The process of claim 1, wherein the microbial cell material comprises whole microbial cells.
Claim 4
Dependent Claim
23 words
The process of claim 1 wherein the microbial cell material is derived from the microorganism of strain NRRL 11240 and contains penicillin V-acylase.
Claim 5
Dependent Claim
24 words
The process of claim 1 wherein the microbial cell material is derived from a strain belonging to the species Bacillus pasteurii and contains urease.
Claim 6
Dependent Claim
36 words
The process of claim 1 wherein the microbial cell material is derived from a strain containing lactase belonging to the species selected from the group consisting of Kluyveromyces fragilis and species of strain NRRL B-11, 229.
Claim 7
Dependent Claim
26 words
The process of claim 1 wherein the microbial cell material is derived from a strain belonging to the species Leuconostoc oenos and contains a malolactic enzyme.
Claim 8
Dependent Claim
23 words
The process of claim 1 wherein the aqueous medium is fermentation broth from cultivation of the microorganism source of the microbial cell material.
Claim 9
Dependent Claim
34 words
The process of claim 1 wherein the aqueous medium is the aqueous part of a cell sludge separated out of the fermentation broth from cultivation of the microorganism source of the microbial cell material.
Claim 10
Dependent Claim
13 words
The process of claim 1 wherein the polyamine is a branched polyethylene imine.
Claim 11
Dependent Claim
19 words
The process of claim 9 wherein the branched polyethylene imine has a molecular weight between 500 and 100.000 Daltons.
Claim 12
Dependent Claim
27 words
The process of claim 1 wherein the process sequence comprises addition of the polyamine to the microbial material containing aqueous medium and subsequently addition to the glutaraldehyde.
Claim 13
Dependent Claim
17 words
The process of claim 1 wherein a ratio glutaraldehyde/microbial cell material (w/w) of from 0.01 to 100.
Claim 14
Dependent Claim
18 words
The process of claim 1 wherein the recovered immobilized enzyme containing microbial cell preparation is shaped by extrusion.
Claim 15
Dependent Claim
28 words
The process of claim 1 wherein the recovered immobilized enzyme containing microbial cell preparation is shaped to a particle size in the range of from 100 to 1000μ.
Claim 16
Dependent Claim
14 words
The immobilized enzyme containing microbial cell preparation produced by the process of claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-09-07 | 1985-03-09 | 1985-09-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-09-07 | 1989-03-09 | 1989-09-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-09-07 | 1993-03-09 | 1993-09-08 | $7700.00 | Window Closed / Lapsed |