US4284724A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of highly concentrated cultivation of yeasts
Assignee: Individual
•
Filing: 1979-01-22
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Granted: 1981-08-18
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Expires: 1999-01-22
Abstract
Abstract A broth containing yeast cells is continuously or intermittently removed from a fermentor. Then, yeast cells are separated from the filtrate using a cell separator, or further washed with water. Then, the yeast cells so obtained are recycled to the fermentor, whereby yeasts are cultivated at a high cell concentration of from 6% to about 20% based on dry weight. As discussed herein, by removing the filtrate from the cultivation system, there is no accumulation of metabolites and salts prohibiting the cultivation of yeasts, and further the growth of miscellaneous microorganisms which interfere with yeast cell growth, is surprisingly suppressed.
Inventors
- 👤 Hideki Fukuda
- 👤 Takeshi Shiotani
- 👤 Wataru Okada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 23:11
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/32; C12N1/14; C12N1/16; C12N1/18; Y; Y10; Y10S; Y10S435/00; Y10S435/80; Y10S435/804; Y10S435/813; Y10S435/8215; Y10S435/911; Y10S435/94
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
174 words
A method of highly concentrated aerobic cultivation of yeast cells in a batch process using a cultivating system comprising a fermentor and a separator, said method comprising: cultivating said yeast cells in said fermentor using a feed liquor comprising carbon source, nutrients and diluting water; setting the starting--up cell concentration at at least 0.1%; removing continuously or intermittently from said fermentor during the course of cultivation, a broth containing a portion of said yeast cells and feed liquor in such a manner that the amount of filtrate removed from said separator is within the range of from 0.3 to 4 times the volume of said feed liquor fed during the course of cultivation; separating said broth into yeast cells and filtrate, said filtrate containing growth inhibiting substances; removing said filtrate from the cultivation system; recycling the total amount of separated yeast cells to said fermentor wherein cultivation is continuing; and repeating the above steps to bring the final cell concentration between 6% and about 20% thereby to substantially suppress growth of undesired miscellaneous microorgaisms.
Claim 2
Independent Claim
174 words
A method of highly concentrated aerobic cultivation of yeast cells in a batch process using a cultivating system comprising a fermentor and a separator, said method comprising: cultivating said yeast cells in said fermentor using a feed liquor comprising carbon source, nutrients and diluting water; setting the starting--up cell concentration at at least 0.1%; removing continuously or intermittently from said fermentor during the course of cultivation, a broth containing a portion of said yeast cells and feed liquor in such a manner that the amount of filtrate removed from said separator is within the range of from 0.3 to 4 times the volume of said feed liquor fed during the course of cultivation; separating said broth into yeast cells and filtrate, said filtrate containing growth inhibiting substances; removing said filtrate from the cultivation system; recycling the total amount of separated yeast cells to said fermentor wherein cultivation is continuing; and repeating the above steps to bring the final cell concentration between 6% and about 20% thereby to substantially suppress growth of undesired miscellaneous microorgaisms.
Claim 3
Dependent Claim
17 words
The method of claim 1, wherein said cell concentration is at least 8% based on dry weight.
Claim 4
Dependent Claim
14 words
The method of claim 1, wherein the starting-up cell concentration is at least 0.5%.
Claim 5
Dependent Claim
33 words
The method of claim 1, wherein said yeast cells are separated centrifugally and washed with water in amounts up to twice the volume of the filtrate remaining after separating of said yeast cells.
Claim 6
Dependent Claim
37 words
The method of claim 1, wherein said yeast cells are separated through a membrane and washed with water in amounts between two to five times the volume of the filtrate remaining after separating of said yeast cells.
Claim 7
Dependent Claim
29 words
The method of claim 1, wherein the yeast is at least one member selected from the group consisting of Saccharomyces; Candida; Rhodotorula; Torulopsis; Hansenula; Pichia; Debaryomyces; Schizosaccharomyces and Trichosporon.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-08-17 | 1985-02-16 | 1985-08-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-08-17 | 1989-02-16 | 1989-08-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-08-17 | 1993-02-16 | 1993-08-18 | $7700.00 | Window Closed / Lapsed |