US4284719A
Expired / Lapsed
US
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Substrate composition and use thereof
Assignee: I-POINT BIOTECHNOLOGIES NYA AGNESFRIDSVAGEN 181 S-213 75 MALMO SWEDEN A SWEDISH JOINT-STOCK Co AB
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Filing: 1980-04-23
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Granted: 1981-08-18
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Expires: 2000-04-23
Abstract
Abstract A substrate composition for reaction with an enzyme, comprising carrier particles and a substrate adsorbed thereto and being characterized in that the carrier consists of polyvinylchloride. Polyvinylchloride of emulsion type is particularly preferred. A preferred substrate is tricaproin which is enzymatically split by lipase enzyme. The use of the substrate composition as a substrate component in enzymatic time-temperature indicating devices is also disclosed.
Inventors
- 👤 Halina Agerhem
- 👤 Hans J. Nilsson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 11:02
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/44; C12Q1/61; G; G01; G01N; G01N31/00; G01N31/22; G01N31/229
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
47 words
A pulverulent enzymatic substrate composition which composition comprises a water-insoluble hydrophobic enzymatic substrate adsorbed on a particulate polyvinyl chloride free of plasticizer and wherein the polyvinyl chloride has an average grain size of at most 0.5 mm, said substrate making up 1-30% by weight of the composition.
Claim 2
Dependent Claim
47 words
A pulverulent enzymatic substrate composition which composition comprises a water-insoluble hydrophobic enzymatic substrate adsorbed on a particulate polyvinyl chloride free of plasticizer and wherein the polyvinyl chloride has an average grain size of at most 0.5 mm, said substrate making up 1-30% by weight of the composition.
Claim 3
Dependent Claim
21 words
A substrate composition as claimed in claim 1, characterized in that the substrate makes up 5-25% by weight of the composition.
Claim 4
Dependent Claim
28 words
A substrate composition as claimed in claim 1, characterized in that the carrier grains consist of agglomerates of primary particles of an average particle size of 0.1-1 μm.
Claim 5
Dependent Claim
21 words
A substrate composition as claimed in claim 1, characterized in that the carrier consists of polyvinyl chloride prepared by emulsion polymerization.
Claim 6
Dependent Claim
20 words
A substrate composition as claimed in claim 1, characterized in that the substrate is selected from liquid and solid triglycerides.
Claim 7
Dependent Claim
15 words
A substrate composition as claimed in claim 5, characterized in that the substrate is glyceryltricapronate.
Claim 8
Dependent Claim
30 words
An aqueous suspension for use in an enzymatic reaction, said suspension comprising (a) a water phase, and (b) the pulverulent substrate composition of claim 1 suspended in said water phase.
Claim 9
Dependent Claim
43 words
An enzymatic time-temperature-integrating device comprising two compartments separated by a rupturable partition, wherein one compartment contains the aqueous suspension of claim 7, and the other compartment contains an enzyme for said substrate, and at least one of the compartments includes a pH indicator.
Claim 10
Dependent Claim
32 words
An enzymatic time-temperature-integrating device comprising a compartment which contains an aqueous solution including an enzyme and a pH indicator, said aqueous solution having suspended therein the pulverulent substrate composition of claim 1.
Claim 11
Dependent Claim
53 words
A method of enzymatic reaction comprising providing an aqueous solution including an enzyme and a pH indicator, combining said aqueous solution with a composition comprising the pulverulent substrate composition of claim 1, reacting said enzyme with said substrate, and determining a change in colour of said solution caused by a change of pH.
Claim 12
Dependent Claim
62 words
A method of determining the activity of an enzyme, comprising providing an aqueous solution including the enzyme and adding to said solution a predetermined amount of the pulverulent substrate composition of claim 1, reacting the enzyme with the substrate while generating an enzymatic reaction product, the determination of enzymatic activity being based on the amount of reaction product produced per time unit.
Claim 13
Dependent Claim
72 words
A method of determining the activity of an enzyme, comprising providing an aqueous solution including the enzyme and adding to said solution a predetermined amount of the pulverulent substrate composition of claim 1, agitating said mixture and maintaining the pH of said mixture constant by the addition of a base or an acid, and determining the activity of the enzyme as the added amount per time unit of said base or acid.
Claim 14
Dependent Claim
14 words
A substrate composition as claimed in claim 1, wherein the composition additionally includes tenside.
Claim 15
Dependent Claim
18 words
A substrate composition as claimed in claim 13, characterized in that it comprises 0.1-3% by weight of tenside.
Claim 16
Dependent Claim
19 words
A substrate composition as claimed in claim 3, characterized in that the composition contains 1-2% by weight of tenside.
References Cited (Backward Citations)
US2671028A
Cited
US3647713A
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US3806417A
Cited
US3822189A
Cited
US4043871A
Cited
US4219334A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-08-17 | 1985-02-16 | 1985-08-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-08-17 | 1989-02-16 | 1989-08-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-08-17 | 1993-02-16 | 1993-08-18 | $7700.00 | Window Closed / Lapsed |