US4279999A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Means for accelerating precipitation in immunoassay and immunoassay method
Assignee: Biodata SpA
•
Filing: 1978-07-20
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Granted: 1981-07-21
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Expires: 1998-07-20
Abstract
Abstract A serum factor extracted through dialysis of an animal serum or anti-serum has been found to accelerate precipitation in radioimmunoassays.
Inventors
- 👤 Giorgio Ricci
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 11:40
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/539; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
77 words
A serum free serum factor capable of accelerating precipitation during radioimmunoassay having the following chemical-physical characteristics: (a) a molecular weight higher than 50,000, (b) being of the Euglobulin type completely insoluble in distilled water, (c) having an electrophoretic mobility which is the same as serum alpha or beta globulins, (d) not assimilated to any component of the serum complement, (e) homogeneous on Sephadex G-100, (f) extracted by dialysis from animal serum or anti-serum, and (g) being thermolabile.
Claim 2
Dependent Claim
77 words
A serum free serum factor capable of accelerating precipitation during radioimmunoassay having the following chemical-physical characteristics: (a) a molecular weight higher than 50,000, (b) being of the Euglobulin type completely insoluble in distilled water, (c) having an electrophoretic mobility which is the same as serum alpha or beta globulins, (d) not assimilated to any component of the serum complement, (e) homogeneous on Sephadex G-100, (f) extracted by dialysis from animal serum or anti-serum, and (g) being thermolabile.
Claim 3
Dependent Claim
9 words
The serum factor of claim 1 in lyophilized form.
Claim 4
Dependent Claim
50 words
Process for obtaining the serum factor claimed in claim 1 comprising dialyzing animal serum or anti-serum against constantly stirred distilled water thereby recovering said serum factor by extraction, centrifuging and washing the solid with distilled water, redissolving in a buffer and then lyophilizing to thereby obtain the lyophilized serum factor.
References Cited (Backward Citations)
US4162895A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-07-20 | 1985-01-19 | 1985-07-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-07-20 | 1989-01-19 | 1989-07-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-07-20 | 1993-01-19 | 1993-07-21 | $7700.00 | Window Closed / Lapsed |