US4277249A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Mammalian Somatomedin measurement
Assignee: Nichols Institute for Endocrinology
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Filing: 1979-10-15
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Granted: 1981-07-07
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Expires: 1999-10-15
Abstract
Abstract Methods and means for collection of blood samples for Somatomedin assay, while suppressing exogenous Somatomedin generation, wherein a Somatomedin-containing blood specimen is absorbed on an absorbent medium and air-dried at ambient temperature, whereafter a portion of the blood-smear containing medium of predetermined uniform dimensions is removed and the Somatomedin activity therein eluted for assay.
Inventors
- 👤 Alan Broughton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 13:56
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
57 words
A method for determining levels of Somatomedin-C in biological fluid which comprises the steps of: (a) collecting a specimen of biological fluid having an unknown content of Somatomedin-C by absorption on an absorbent medium; (b) drying the absorbent medium; (c) eluting the Somatomedin-C activity from the absorbent medium; and (d) determining the Somatomedin-C activity of the eluate.
Claim 2
Independent Claim
57 words
A method for determining levels of Somatomedin-C in biological fluid which comprises the steps of: (a) collecting a specimen of biological fluid having an unknown content of Somatomedin-C by absorption on an absorbent medium; (b) drying the absorbent medium; (c) eluting the Somatomedin-C activity from the absorbent medium; and (d) determining the Somatomedin-C activity of the eluate.
Claim 3
Dependent Claim
23 words
A method as claimed in claim 1 wherein the activity is eluted from a portion of the medium of predetermined size and dimension.
Claim 4
Independent Claim
40 words
A method for suppressing exogenous generation of Somatomedin-C in biological fluid specimens, which comprises the steps of: (a) collecting a fluid specimen having an unknown content of Somatomedin-C; (b) absorbing the fluid on an absorbent medium; (c) drying the medium.
Claim 5
Independent Claim
40 words
A method for suppressing exogenous generation of Somatomedin-C in biological fluid specimens, which comprises the steps of: (a) collecting a fluid specimen having an unknown content of Somatomedin-C; (b) absorbing the fluid on an absorbent medium; (c) drying the medium.
Claim 6
Dependent Claim
16 words
A method as claimed in claim 1 or 3 wherein the absorbent medium is filter paper.
Claim 7
Dependent Claim
20 words
A method as claimed in claim 1 or 3 wherein the drying of the medium is done at ambient temperature.
Claim 8
Dependent Claim
20 words
A method as claimed in claim 1 or 3 wherein the drying of the medium is done at room temperature.
Claim 9
Dependent Claim
18 words
A method as claimed in claim 1 or 3 wherein the biological fluid is a species of blood.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-07-06 | 1985-01-05 | 1985-07-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-07-06 | 1989-01-05 | 1989-07-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-07-06 | 1993-01-05 | 1993-07-07 | $7700.00 | Window Closed / Lapsed |