US4275159A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the production of xylose by enzymatic hydrolysis of xylan
Assignee: Sunopta Inc
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Filing: 1979-07-30
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Granted: 1981-06-23
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Expires: 1999-07-30
Abstract
Abstract A process for the production of xylose by enzymatic hydrolysis of xylan wherein an aqueous solution containing xylan is treated with a carrier having bonded thereto xylanase enzyme and a carrier having bonded thereto β-xylosidase and, optionally, uronic acid-splitting enzyme.
Inventors
- 👤 Jurgen Puls
- 👤 Michael Sinner
- 👤 Hans-Hermann Dietrichs
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 04:35
CPC Classifications
C; C13; C13K; C13K13/00; C13K13/002; Y; Y10; Y10S; Y10S435/00; Y10S435/814
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
145 words
A process for the immobilization of purified xylanase, β-xylosidase, and uronic acid-splitting enzymes on to carriers comprising, (a) dissolving an untreated enzyme in a buffer solution having a pH between about 4 and about 6, (b) freeing said enzyme solution from insoluble constituents, (c) filtering the solution obtained from step (b) through an ultrafilter having a cut-off between about MW 80,000 and about MW 120,000 and collecting both the supernatant and the filtrate, (d) filtering the supernatant through an ultrafilter having a cut-off between about MW 250,000 and about MW 350,000 and recovering the filtrate containing substantially β-xylosidase and uronic acid-splitting enzymes, if present, (e) filtering the filtrate obtained in step (c) through an ultrafilter having a cut-off between about MW 10,000 and about MW 50,000 and recovering the filtrate containing substantially xylanase, (f) bonding the recovered filtrates from steps (d) and (e) to carriers.
Claim 2
Dependent Claim
145 words
A process for the immobilization of purified xylanase, β-xylosidase, and uronic acid-splitting enzymes on to carriers comprising, (a) dissolving an untreated enzyme in a buffer solution having a pH between about 4 and about 6, (b) freeing said enzyme solution from insoluble constituents, (c) filtering the solution obtained from step (b) through an ultrafilter having a cut-off between about MW 80,000 and about MW 120,000 and collecting both the supernatant and the filtrate, (d) filtering the supernatant through an ultrafilter having a cut-off between about MW 250,000 and about MW 350,000 and recovering the filtrate containing substantially β-xylosidase and uronic acid-splitting enzymes, if present, (e) filtering the filtrate obtained in step (c) through an ultrafilter having a cut-off between about MW 10,000 and about MW 50,000 and recovering the filtrate containing substantially xylanase, (f) bonding the recovered filtrates from steps (d) and (e) to carriers.
Claim 3
Dependent Claim
42 words
A process according to claim 1, wherein the filtrate containing substantially all xylanase is filtered through an ultrafilter with a cut-off range of at least about MW 300 and at most about MW 700, and the residue is bonded onto the carrier.
Claim 4
Dependent Claim
19 words
A process according to claim 1, wherein the carriers are activated with glutaraldehyde, cyclohexylmorpholinoethylcarbodiimide-toluenesulfonate (CMC) or TiCl 4 .
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-22 | 1984-12-22 | 1985-06-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-22 | 1988-12-22 | 1989-06-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-22 | 1992-12-22 | 1993-06-23 | $7700.00 | Window Closed / Lapsed |