Immune serum for diagnosis of cancer and preparation thereof

Claim 1

A specific diagnostic immune serum for cancer diagnosis prepared by immunizing a non-human animal with an acid protein composition having immunosuppressive properties (IAP) having an isoelectric point pH of 2.9 to 3.3 determined by thin layer gel electrofocusing and a molecular weight of about 59,000 determined by electrophoresis with 2.5% polyacrylamide gel containing sodium dodecyl sulfate, said acid protein compositions being a white powder exhibiting the following properties: decomposition point 265° C. soluble in water and in 0.01 M phosphate buffered saline insoluble in methanol, ethanol, butanol, acetone, ethylacetate and chloroform positive color reaction with von Euler's reagent, Ehrlich's reagent, Buret's reagent and Molisch's reagent negative color reaction with ferric chloride reagent and Lieberman's reagent positive reaction to ninhydrin after hydrolysis in 6 N hydrolchloric acid at 120° C. for 15 hours infrared absorption spectrum with 0.5% K Br showing maxima at 3300 cm -1 , 2940 cm -1 , 2340 cm -1 , 1650 cm -1 , 1540 cm -1 , 1440 cm -1 , 1400 cm -1 , 1240 cm -1 , 1060 cm -1 , ultra-violet absorption spectrum of an 0.02% solution in distilled water λmax=280 nm and which is obtainable by negative ion-exchange chromatography from a human body liquid selected from the group consisting of serum, peritoneal fluid, thoracic fluid, urine and other body liquids of humans suffering from cancer and the liquid extracted from placentas of healthy humans.

Claim 3

A method for preparing a specific diagnostic immune serum for cancer diagnosis which comprises the steps of: (a) preparing a solution in an aqueous injectable medium of an acid protein composition having immunosuppressive properties (IAP) having an isoelectric point pH of 2.9 to 3.3 determined by thin layer gel electrofocusing and a molecular weight of about 59,000 determined by electrophoresis with 7.5% polyacrylamide gel containing sodium dodecyl sulfate, said acid protein composition being a white powder exhibiting the following properties: decomposition point 265° C. soluble in water and in 0.01 M phosphate buffered saline insoluble in methanol, ethanol, butanol, acetone, ethylacetate and chloroform positive color reaction with von Euler's reagent, Ehrlich's reagent, Buret's reagent and Molisch's reagent negative color reaction with ferric chloride reagent and Lieberman's reagent positive reaction to ninhydrin after hydrolysis natant IAP antibody-containing liquid phase; and (i) separating the supernatant IAP anti-body-containing liquid from the sediment.

Claim 4

A method for preparing a specific diagnostic immune serum for cancer diagnosis which comprises the steps of: (a) preparing a solution in an aqueous injectable medium of an acid protein composition having immunosuppressive properties (IAP) having an isoelectric point pH of 2.9 to 3.3 determined by thin layer gel electrofocusing and a molecular weight of about 59,000 determined by electrophoresis with 7.5% polyacrylamide gel containing sodium dodecyl sulfate, said acid protein composition being a white powder exhibiting the following properties: decomposition point 265° C. soluble in water and in 0.01 M phosphate buffered saline insoluble in methanol, ethanol, butanol, acetone, ethylacetate and chloroform positive color reaction with von Euler's reagent, Ehrlich's reagent, Buret's reagent and Molisch's reagent negative color reaction with ferric chloride reagent and Lieberman's reagent positive reaction to ninhydrin after hydrolysis natant IAP antibody-containing liquid phase; and (i) separating the supernatant IAP anti-body-containing liquid from the sediment.