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US4266034A Expired / Lapsed US ⏱️ 0.0 years remaining

Method for producing microbial cells and use thereof to produce oxidation products

Assignee: ExxonMobil Technology and Engineering Co
•
Filing: 1979-03-27
•
Granted: 1981-05-05
•
Expires: 1999-03-27
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Abstract

Abstract Disclosed are newly discovered and isolated methylotrophic microorganism strains and their natural and/or artificial mutants which grow well under aerobic conditions in a culture medium in the presence of methane as the major carbon and energy source. The methane-grown microbial cells possess a high content of protein and can be utilized as such as feedstuffs. The methane-grown microbial cells or enzyme preparations thereof are also useful in converting C1-C6 alkanes to alcohols, particularly methane to methanol, C3-C6 alkanes to the corresponding C3-C6 sec. alcohols and methyl ketones, C3-C6 sec. alcohols to the corresponding methyl ketones, cyclic hydrocarbons to cyclic hydrocarbyl alcohols (e.g., cyclohexane to cyclohexanol) C2-C4 alkenes selected from the group consisting of ethylene, propylene, butene-1 and butadiene to 1,2-epoxides, styrene to styrene oxide, and converting other oxidizable substrates to oxidized products. The newly discovered and isolated methylotrophic microorganism strains may be aerobically grown on a plurality of methyl-radical donating carbon-containing compounds, such as methanol, methylamine, methyl formate, methyl carbonate, dimethyl ether, etc., to produce microbial cells or enzyme preparations capable of aerobically converting C3-C6 linear secondary alcohols to the corresponding methyl ketones.

Inventors

  • 👤 Ramesh N. Patel
  • 👤 Ching-Tsang Hou
  • 👤 Allen I. Laskin

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-08 16:06
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0071; C12N9/0073; C07; C07D; C07D301/00; C07D301/02; C07D303/00; C07D303/02; C07D303/04; C12N1/00; C12N1/26; C12N1/28; C12N1/30; C12N9/0006; C12P; C12P17/00; C12P17/02; C12P7/00; C12P7/02; C12P7/04; C12P7/06; C12P7/24; C12P7/26; C12P7/28; C12Y; C12Y114/00; C12Y114/13; C12Y114/13025; Y; Y02; Y02E; Y02E50/00; Y02E50/10; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/858

Claims Analysis

Total Claims: 4
Claim 1
Independent Claim 242 words
Isolated and biologically pure microbial cultures of C 1 compound utilizing microorganisms, said cultures being selected from the group consisting of those having the following identifying characteristics: ______________________________________ Methylosinus trichosporium (CRL 15 PM1) NRRL B-11,202 Methylosinus sporium (CRL 16 PM2) NRRL B-11,203 Methylocystis parvus (CRL 18 PM4) NRRL B-11,204 Methylomonas methanica (CRL M4P) NRRL B-11,205 Methylomonas methanica (CRL 21 PM7) NRRL B-11,206 Methylomonas albus (CRL M8Y) NRRL B-11,207 Methylomonas streptobacterium (CRL 17 PM3) NRRL B-11,208 Methylomonas agile (CRL 22 PM9) NRRL B-11,209 Methylomonas rubrum (CRL M6P) NRRL B-11,210 Methylomonas rubrum (CRL 20 PM6) NRRL B-11,211 Methylomonas rosaceus (CRL M10P) NRRL B-11,212 Methylomonas rosaceus (CRL M7P) NRRL B-11,213 Methylobacter chroococcum (CRL M6) NRRL B-11,214 Methylobacter chroococcum (CRL 23 PM8) NRRL B-11,215 Methylobacter bovis (CRL M1Y) NRRL B-11,216 Methylobacter bovis (CRL 19 PM5) NRRL B-11,217 Methylobacter vinelandii (CRL M5Y) NRRL B-11,218 Methylococcus capsulatus (CRL M1) NRRL B-11,219 Methylococcus minimus (CRL 24 PM12) NRRL B-11,220 Methylococcus capsulatus (CRL 25 PM13) NRRL B-11,221 Methylobacterium organophilum (CRL 26 R6) NRRL B-11,222 Pichia sp. (CRL-72) NRRL Y-11,328 Torulopsis sp. (A1) NRRL Y-11,419 Kloeckera sp. (A2) NRRL Y-11,420 ______________________________________ and mutants thereof, said cultures capable of reproducing themselves and capable of producing secondary alcohol dehydrogenase or monooxygenase enzyme activity in isolatable amounts when cultured under aerobic conditions in a liquid growth medium comprising assimilable sources of nitrogen and essential mineral salts in the presence of methane or a methyl radical donating compound as the major carbon and energy source.
Claim 2
Dependent Claim 242 words
Isolated and biologically pure microbial cultures of C 1 compound utilizing microorganisms, said cultures being selected from the group consisting of those having the following identifying characteristics: ______________________________________ Methylosinus trichosporium (CRL 15 PM1) NRRL B-11,202 Methylosinus sporium (CRL 16 PM2) NRRL B-11,203 Methylocystis parvus (CRL 18 PM4) NRRL B-11,204 Methylomonas methanica (CRL M4P) NRRL B-11,205 Methylomonas methanica (CRL 21 PM7) NRRL B-11,206 Methylomonas albus (CRL M8Y) NRRL B-11,207 Methylomonas streptobacterium (CRL 17 PM3) NRRL B-11,208 Methylomonas agile (CRL 22 PM9) NRRL B-11,209 Methylomonas rubrum (CRL M6P) NRRL B-11,210 Methylomonas rubrum (CRL 20 PM6) NRRL B-11,211 Methylomonas rosaceus (CRL M10P) NRRL B-11,212 Methylomonas rosaceus (CRL M7P) NRRL B-11,213 Methylobacter chroococcum (CRL M6) NRRL B-11,214 Methylobacter chroococcum (CRL 23 PM8) NRRL B-11,215 Methylobacter bovis (CRL M1Y) NRRL B-11,216 Methylobacter bovis (CRL 19 PM5) NRRL B-11,217 Methylobacter vinelandii (CRL M5Y) NRRL B-11,218 Methylococcus capsulatus (CRL M1) NRRL B-11,219 Methylococcus minimus (CRL 24 PM12) NRRL B-11,220 Methylococcus capsulatus (CRL 25 PM13) NRRL B-11,221 Methylobacterium organophilum (CRL 26 R6) NRRL B-11,222 Pichia sp. (CRL-72) NRRL Y-11,328 Torulopsis sp. (A1) NRRL Y-11,419 Kloeckera sp. (A2) NRRL Y-11,420 ______________________________________ and mutants thereof, said cultures capable of reproducing themselves and capable of producing secondary alcohol dehydrogenase or monooxygenase enzyme activity in isolatable amounts when cultured under aerobic conditions in a liquid growth medium comprising assimilable sources of nitrogen and essential mineral salts in the presence of methane or a methyl radical donating compound as the major carbon and energy source.
Claim 3
Dependent Claim 10 words
The cultures of claim 1 wherein the cultures are bacteria.
Claim 4
Dependent Claim 10 words
The cultures of claim 1 wherein the cultures are yeasts.

References Cited (Backward Citations)

US3326771A Cited
US3344037A Cited
US3930947A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1984-05-04 1984-11-03 1985-05-05 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1988-05-04 1988-11-03 1989-05-05 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1992-05-04 1992-11-03 1993-05-05 $7700.00 Window Closed / Lapsed

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