US4259232A
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Flavin adenine dinucleotide-labeled protein and polypeptide conjugates
Assignee: Bayer Corp
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Filing: 1979-10-23
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Granted: 1981-03-31
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Expires: 1999-10-23
Abstract
Abstract Flavin adenine dinucleotide-labeled conjugates of the formula: <IMAGE> wherein Riboflavin-Phos)2 Ribose represents the riboflavin-pyrophosphate-ribose residue in flavin adenine dinucleotide (FAD), -NH)L is a protein or polypeptide (e.g., an immunoglobulin) bound through an amino group thereof, n is an integer from 2 through 10, m is an integer from 1 through 10, and p is on the average from 1 to the number of available amino groups in L. The FAD-labeled conjugates are useful as reagents in specific binding assays (e.g., immunoassays) to determine the conjugated protein or polypeptide, or a specific binding analog or partner thereof, in liquid media.
Inventors
- 👤 Robert J. Carrico
- 👤 That T. Ngo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 23:02
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S530/00; Y10S530/806; Y10S530/861; Y10S530/863
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
83 words
A flavin adenine dinucleotide-labeled conjugate of the formula: ##STR7## wherein Riboflavin --Phos) 2 Ribose represents the riboflavin-pyrophosphate-ribose residue in flavin adenine dinucleotide, --NH)L is a protein or polypeptide bound through an amino group thereof whereby said protein or polypeptide in the conjugate is capable of being bound by an antibody, n is an integer from 2 through 10, m is an integer from 1 through 10, and p is on the average from 1 to the number of available amino groups in L.
Claim 2
Dependent Claim
83 words
A flavin adenine dinucleotide-labeled conjugate of the formula: ##STR7## wherein Riboflavin --Phos) 2 Ribose represents the riboflavin-pyrophosphate-ribose residue in flavin adenine dinucleotide, --NH)L is a protein or polypeptide bound through an amino group thereof whereby said protein or polypeptide in the conjugate is capable of being bound by an antibody, n is an integer from 2 through 10, m is an integer from 1 through 10, and p is on the average from 1 to the number of available amino groups in L.
Claim 3
Dependent Claim
19 words
The conjugate of claim 1 wherein --NH)L is a protein or polypeptide of molecular weight between 130 and 10,000,000.
Claim 4
Dependent Claim
19 words
The conjugate of claim 1 wherein --NH)L is a protein or polypeptide of molecular weight between 1,000 and 1,000,000.
Claim 5
Dependent Claim
10 words
The conjugate of claim 1 wherein --NH)L is an immunoglobulin.
Claim 6
Dependent Claim
10 words
The conjugate of claim 4 wherein said immunoglobulin is IgG.
Claim 7
Dependent Claim
10 words
The conjugate of claim 4 wherein said immunoglobulin is IgM.
Claim 8
Dependent Claim
10 words
The conjugate of claim 4 wherein said immunoglobulin is IgA.
Claim 9
Dependent Claim
17 words
The conjugate of any of claims 1-7 wherein p is on the average from 1 to 100.
Claim 10
Dependent Claim
17 words
The conjugate of any of claims 1-7 wherein p is on the average from 1 to 20.
Claim 11
Dependent Claim
9 words
The conjugate of claim 9 wherein n=6 and m=4.
Claim 12
Dependent Claim
11 words
The conjugate of any of claims 1-7 wherein n=6 and m=4.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-30 | 1984-09-29 | 1985-03-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-30 | 1988-09-29 | 1989-03-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-30 | 1992-09-29 | 1993-03-31 | $7700.00 | Window Closed / Lapsed |