US4258134A
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US
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Novel hyaluronidase BMP-8231 and production thereof
Assignee: Astellas Pharma Inc
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Filing: 1979-05-03
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Granted: 1981-03-24
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Expires: 1999-05-03
Abstract
Abstract Hyaluronidase obtained from Streptomyces koganeiensis has the following characteristics: (a) Acts as an endo B-hexosaminidase (b) Degrades hyaluronic acid but not chondroitin sulfate A, C, or chondroitin (c) Optimum pH around 4.0 (d) Stable pH
Inventors
- 👤 Keizo Yoshida
- 👤 Takashi Fujii
- 👤 Hiroyuki Kikuchi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 17:25
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2408; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
60 words
Hyaluronidase BMP-8231 having the characteristic of (a) Type of Action: Endo β-hexosaminidase; (b) Substrate Specificity: Degradable: Hyaluronic acid Not Degradable: Chondroitin sulfate A, Chondroitin sulfate C and Chondroitin; (c) Optimum pH: Around pH 4.0; (d) Stable pH range: 4.0-pH 11.3; (e) Optimum temperature: Around 60° C.; (f) Stable temperature range: Below 60° C.; and (g) Stability against proteolytic enzyme: Stable.
Claim 2
Dependent Claim
60 words
Hyaluronidase BMP-8231 having the characteristic of (a) Type of Action: Endo β-hexosaminidase; (b) Substrate Specificity: Degradable: Hyaluronic acid Not Degradable: Chondroitin sulfate A, Chondroitin sulfate C and Chondroitin; (c) Optimum pH: Around pH 4.0; (d) Stable pH range: 4.0-pH 11.3; (e) Optimum temperature: Around 60° C.; (f) Stable temperature range: Below 60° C.; and (g) Stability against proteolytic enzyme: Stable.
Claim 3
Dependent Claim
28 words
A process for preparing hyaluronidase BMP-8231, which comprises culturing a strain of Streptomyces koganeiensis in a nutrient medium and recovering the said hyaluronidase BMP-8231 from the cultured broth.
Claim 4
Dependent Claim
28 words
A process for preparing hyaluronidase BMP-8231, which comprises culturing a strain of Streptomyces koganeiensis in a nutrient medium and recovering the said hyaluronidase BMP-8231 from the cultured broth.
Claim 5
Dependent Claim
15 words
The process according to claim 2, in which the strain is Streptomyces koganeiensis ATCC 31394.
Claim 6
Dependent Claim
9 words
Hyaluronidase BMP-8231 obtained by the process of claim 3.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-23 | 1984-09-22 | 1985-03-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-23 | 1988-09-22 | 1989-03-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-23 | 1992-09-22 | 1993-03-24 | $7700.00 | Window Closed / Lapsed |