US4256588A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Separation and recovery of B and T lymphocytes
Assignee: EIDP Inc
•
Filing: 1979-11-02
•
Granted: 1981-03-17
•
Expires: 1999-11-02
Abstract
Abstract A process for the separation of B and T lymphocytes comprising passing a suspension of a mixture over a bed of synthetic polymer granules whereby the B leukocytes adhere to the granules and the T lymphocytes pass through the bed.
Inventors
- 👤 Harvey H. Hoehn
- 👤 Stephen T. Toy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:52
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/17
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
61 words
A process comprising passing an aqueous suspension of a mixture of B lymphocytes and T lymphocytes through a bed of synthetic polymeric granules having a diameter of from 0.1 to 5 mm at a temperature of from about 4° C. to about 25° C. whereby the B lymphocytes become adhered to the granules and the T lymphocytes pass through the bed.
Claim 2
Dependent Claim
61 words
A process comprising passing an aqueous suspension of a mixture of B lymphocytes and T lymphocytes through a bed of synthetic polymeric granules having a diameter of from 0.1 to 5 mm at a temperature of from about 4° C. to about 25° C. whereby the B lymphocytes become adhered to the granules and the T lymphocytes pass through the bed.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein the pH of the suspension is from 6.9 to 7.5.
Claim 4
Dependent Claim
15 words
The process of claim 2 wherein the suspension contains from 1 to 10 percent serum.
Claim 5
Dependent Claim
23 words
The process of claim 3 wherein the granules are made of a synthetic polymer selected from the class consisting of polyamides and polyesters.
Claim 6
Dependent Claim
12 words
The process of claim 4 wherein the synthetic polymer is a polyamide.
Claim 7
Dependent Claim
11 words
The process of claim 5 wherein the polyamide is polyhexamethylene adipamide.
Claim 8
Dependent Claim
12 words
The process of claim 4 wherein the synthetic polymer is a polyester.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-16 | 1984-09-15 | 1985-03-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-16 | 1988-09-15 | 1989-03-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-16 | 1992-09-15 | 1993-03-17 | $7700.00 | Window Closed / Lapsed |