US4255267A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Separation and recovery of granulocytes from blood using adherence on an expandable bed of a polymeric material
Assignee: EIDP Inc
•
Filing: 1979-11-02
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Granted: 1981-03-10
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Expires: 1999-11-02
Abstract
Abstract A leukapheresis process is disclosed in which an expandable bed of synthetic polymeric packing having a diameter of from 90 μm to 2000 μm and preferably 200 μm to 400 μm is used to adhere granulocytes from blood. The granulocytes are harvested from the packing by expanding the bed and washing with a physiologically acceptable solution.
Inventors
- 👤 Harvey H. Hoehn
- 👤 John R. Wells
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 00:36
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/15; B; B01; B01D; B01D15/00; B01D15/08; A61M; A61M1/00; A61M1/02; A61M1/0272
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
58 words
A process comprising passing blood through an expandable bed packed with synthetic normally solid polymer having a smooth surface and an average diameter of from about 90 μm to about 2000 μm whereby granulocytes become adhered to the packing, expanding the bed from 25% to 600%, and washing the granulocytes off the packing with a physiologically acceptable solution.
Claim 2
Dependent Claim
58 words
A process comprising passing blood through an expandable bed packed with synthetic normally solid polymer having a smooth surface and an average diameter of from about 90 μm to about 2000 μm whereby granulocytes become adhered to the packing, expanding the bed from 25% to 600%, and washing the granulocytes off the packing with a physiologically acceptable solution.
Claim 3
Dependent Claim
24 words
The process of claim 1 wherein the packing is made of a resin selected from the class consisting of nylon, polymethyl methacrylate, and polyesters.
Claim 4
Dependent Claim
12 words
The process of claim 2 wherein the packing is beads or rods.
Claim 5
Dependent Claim
16 words
The process of claim 3 wherein the packing is beads, which beads are made of nylon.
Claim 6
Dependent Claim
11 words
The process of claim 4 wherein the nylon is poly(hexamethylene adipamide).
Claim 7
Dependent Claim
18 words
The process of claim 5 wherein the beads have an average diameter of from 150 to 400 μm.
Claim 8
Dependent Claim
21 words
The process of claim 1 wherein the bed is beads which bed is inverted to wash the granulocytes from the beads.
Claim 9
Dependent Claim
24 words
The process of claim 7 wherein the beads are made of a resin selected from the class consisting of nylon, polymethyl methacrylate, and polyesters.
Claim 10
Dependent Claim
12 words
The process of claim 8 wherein the beads are made of nylon.
Claim 11
Dependent Claim
11 words
The process of claim 9 wherein the nylon is poly(hexamethylene adipamide).
Claim 12
Dependent Claim
18 words
The process of claim 10 wherein the beads have an average diameter of from 150 to 400 μm.
References Cited (Backward Citations)
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Cited
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-09 | 1984-09-08 | 1985-03-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-09 | 1988-09-08 | 1989-03-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-09 | 1992-09-08 | 1993-03-10 | $7700.00 | Window Closed / Lapsed |