US4254219A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for recording data in the leucocyte migration inhibition assay
Assignee: Merck and Co Inc
•
Filing: 1979-09-21
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Granted: 1981-03-03
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Expires: 1999-09-21
Abstract
Abstract A method of detecting and quantitating the migration of leucocytes in agar gel by (1) fixing the cells; (2) staining them with Ponceau S or a similar dye; (3) destaining; and (4) dissolving the gel by gentle heating in the destaining solution. The stained cells remain adhered to the glass surface of the culture dish, and the cells can be read through a projection microscope or photographed for a permanent record.
Inventors
- 👤 W. Wardle Fullerton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 00:16
CPC Classifications
G; G01; G01N; G01N1/00; G01N1/28; G01N1/30; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5094; G01N2001/305; G01N2474/00; G01N2474/20
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
43 words
In an antigen-induced leucocyte migration assay wherein leucocytes are added to a hole in an agar medium, the improvement which comprises fixing and staining the leucocytes after formation of a precipitin band, and heating the system to permit removal of the agar medium.
Claim 2
Dependent Claim
43 words
In an antigen-induced leucocyte migration assay wherein leucocytes are added to a hole in an agar medium, the improvement which comprises fixing and staining the leucocytes after formation of a precipitin band, and heating the system to permit removal of the agar medium.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein the stain is a protein stain.
Claim 4
Dependent Claim
26 words
The method of claim 2 in which the stain used is a staining and fixing solution of 0.2% Ponceau S in 5% trichloracetic acid in water.
Claim 5
Dependent Claim
16 words
The method of claim 1 wherein the system is washed before heating with a solvent system.
Claim 6
Dependent Claim
18 words
The method of claim 4 in which the washing solvent system is 7.5/5.0/87.5 acetic acid, methanol and water.
Claim 7
Dependent Claim
16 words
The method of claim 5 in which the heating is at 50°-60° C. for 2-3 hours.
References Cited (Backward Citations)
US3960489A
Cited
US3960490A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-02 | 1984-09-01 | 1985-03-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-02 | 1988-09-01 | 1989-03-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-02 | 1992-09-01 | 1993-03-03 | $7700.00 | Window Closed / Lapsed |