US4251631A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cross-linked enzyme membrane
Assignee: RESEARCH PRODUCTS REHOVOT LTDKIRYAT WEIZMANN
•
Filing: 1979-02-01
•
Granted: 1981-02-17
•
Expires: 1999-02-01
Abstract
Abstract A cross-linked enzyme membrane is prepared by directly adsorbing enzymes into the pores of a microporous non-fibrous filter membrane made of a silica modified vinylchloride polymer and then cross-linking the enzyme with a bi-functional coupling agent whereby enzyme molecules are cross-linked to each other without chemically bonding the enzyme molecules to the membrane. The cross-linked enzyme membrane is used to carry out enzymatic reactions by passing a solution of substrate for the enzyme through the membrane under a pressure differential.
Inventors
- 👤 Shulamith Simon
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 15:15
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12P19/00; C12P19/14
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
142 words
A method for effecting an enzymatic reaction which comprises causing an aqueous solution of a substrate capable of being chemically altered upon contact with an enzyme to flow under a pressure differential through a cross-linked enzyme membrane, wherein said cross-linked enzyme membrane is a microporous non-fibrous filter membrane having a pore size of between about 0.1 and 2.0 microns and made of a silica modified vinylchloride polymer selected from the group consisting of polyvinyl chloride, vinylchloride-propylene copolymer and vinylchloride-vinyl acetate copolymer, and said cross-linked membrane is formed by directly adsorbing said enzyme into the pores of said microporous nonfibrous filter membrane and then cross-linking the enzyme therein by means of a bi-functional coupling agent, whereby enzyme molecules in the pores are cross-linked to each other but are not chemically bound directly or through a bridging group to the microporous non-fibrous filter membrane.
Claim 2
Independent Claim
142 words
A method for effecting an enzymatic reaction which comprises causing an aqueous solution of a substrate capable of being chemically altered upon contact with an enzyme to flow under a pressure differential through a cross-linked enzyme membrane, wherein said cross-linked enzyme membrane is a microporous non-fibrous filter membrane having a pore size of between about 0.1 and 2.0 microns and made of a silica modified vinylchloride polymer selected from the group consisting of polyvinyl chloride, vinylchloride-propylene copolymer and vinylchloride-vinyl acetate copolymer, and said cross-linked membrane is formed by directly adsorbing said enzyme into the pores of said microporous nonfibrous filter membrane and then cross-linking the enzyme therein by means of a bi-functional coupling agent, whereby enzyme molecules in the pores are cross-linked to each other but are not chemically bound directly or through a bridging group to the microporous non-fibrous filter membrane.
Claim 3
Dependent Claim
18 words
A method according to claim 1, wherein said pressure differential is from about 0.05 to about 5 atmospheres.
Claim 4
Dependent Claim
14 words
A method according to claim 1 wherein the enzymatic reaction is a hydrolytic reaction.
Claim 5
Dependent Claim
39 words
A method according to claim 3, wherein said hydrolytic reaction comprises the selective hydrolysis of one isomer of a racemic mixture of amino acid compounds having at least one of an acylated amino group and an esterified carboxylic group.
Claim 6
Dependent Claim
14 words
A method according to claim 1, wherein the enzymatic reaction is an oxidizing reaction.
Claim 7
Dependent Claim
13 words
A method according to claim 1, wherein said enzyme is Chymotrypsin or Trypsin.
Claim 8
Dependent Claim
14 words
A method according to claim 1 wherein said membrane is packed in a cartridge.
Claim 9
Dependent Claim
35 words
A method according to claim 7 wherein said membrane is pleated and packed in a cartridge having a packing ratio of membrane area to cartridge volume of at least 5 cm 2 /cm 3 .
Claim 10
Dependent Claim
23 words
A method according to claim 1, wherein the cross-linking agent is selected from the group consisting of dialdehydes, dioxobenzidine, hexamethylene-diisocyanate, 1,5-difluoro-2,4-dinitrobenzene and N,N'-hexamethylene-bisiodoacetamide.
Claim 11
Dependent Claim
12 words
A method according to claim 9, wherein the cross-linking agent is glutaraldehyde.
Claim 12
Dependent Claim
108 words
An immobilized-enzyme flow-through reactor suitable for effecting an enzymatic reaction comprising a microporous non-fibrous filter membrane having a pore size of between about 0.1 to 2.0 microns and made of a silica modified vinylchloride polymer selected from the group consisting of polyvinyl chloride, vinyl chloride-propylene copolymer and vinyl chloride-vinyl acetate copolymer and having an enzyme immobilized in the pores thereof by directly adsorbing the enzyme in the pores and then cross-linking the enzyme by means of a bi-functional coupling agent, whereby enzyme molecules in the pores are cross-linked to each other but are not chemically bound directly or through a bridging group to the microporous non-fibrous filter membrane.
Claim 13
Dependent Claim
108 words
An immobilized-enzyme flow-through reactor suitable for effecting an enzymatic reaction comprising a microporous non-fibrous filter membrane having a pore size of between about 0.1 to 2.0 microns and made of a silica modified vinylchloride polymer selected from the group consisting of polyvinyl chloride, vinyl chloride-propylene copolymer and vinyl chloride-vinyl acetate copolymer and having an enzyme immobilized in the pores thereof by directly adsorbing the enzyme in the pores and then cross-linking the enzyme by means of a bi-functional coupling agent, whereby enzyme molecules in the pores are cross-linked to each other but are not chemically bound directly or through a bridging group to the microporous non-fibrous filter membrane.
Claim 14
Dependent Claim
16 words
An immobilized-enzyme flow-through reactor according to claim 11 wherein said membrane is packed in a cartridge.
Claim 15
Dependent Claim
37 words
An immobilized-enzyme flow-through reactor according to claim 12 wherein said membrane is pleated and packed in a cartridge having a packing ratio of membrane area to cartridge volume of at least 5 cm 2 /cm 3 .
Claim 16
Dependent Claim
25 words
An immobilized-enzyme flow-through reactor according to claim 11 wherein the cross-linking agent is selected from the group consisting of dialdehydes, dioxobenzidine, hexamethylene-diisocyanate, 1,5-difluoro-2,4-dinitrobenzene and N,N'-hexamethylene-bisiodoacetamide.
Claim 17
Dependent Claim
14 words
An immobilized-enzyme flow-through reactor according to claim 14, wherein the cross-linking agent is glutaraldehyde.
References Cited (Backward Citations)
GB1183260A
Cited
US3705084A
Cited
US3796634A
Cited
US3977941A
Cited
US3804719A
Cited
US4102746A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-02-17 | 1984-08-18 | 1985-02-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-02-17 | 1988-08-18 | 1989-02-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-02-17 | 1992-08-18 | 1993-02-17 | $7700.00 | Window Closed / Lapsed |