US4243753A
Expired / Lapsed
US
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Apparatus for enzyme detection
Assignee: Purdue Research Foundation
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Filing: 1978-02-27
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Granted: 1981-01-06
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Expires: 1998-02-27
Abstract
Abstract An apparatus is disclosed for enzyme detection that is particularly well suited for high performance liquid chromatography. The apparatus is basically a flow-through enzyme detector that is capable of maintaining zero-order reaction kinetics with respect to substrate during enzyme detection and this results in a linear accumulation of product that can be readily measured to provide a dependable readout of detected enzymes. A sample in liquid form that may include one or more enzymes is coupled through a separation column to separate the enzymes, after which a reaction causing substrate is added and the resulting mixture is caused to flow through a reaction chamber, reaction products then being detected with the readout therefrom indicating the detected enzymes. The dimensions of the reaction chamber are dependent upon flow rates and must be selected to allow the mixture to remain in the chamber for a preselected reaction time. In addition, the chamber and packing material therein are selected to assure minimization of chromatographic profile distortion by band spreading as well as preventing separation of the components of the mixture passing therethrough. To this end, the chamber and packing material within the chamber must be of non-porous material, inert to proteins to be detected, and must assure even flow conditions.
Inventors
- 👤 Frederick E. Regnier
- 👤 Shung-Ho Chang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 23:42
CPC Classifications
C; C12; C12M; C12M21/00; C12M21/18; C12M25/00; C12M25/16; C12M27/00; C12M33/00; C12M33/04; C12M47/00; C12M47/10; C12M47/12; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/808; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
203 words
A detection apparatus, comprising: mixing means for receiving and mixing, in liquid form, a compound having at least one enzyme to be detected and reacting material for said enzyme; fluid inlet means for receiving, in liquid form, said mixed compound and reacting material from said mixing means; reaction enabling means including a chamber substantially filled with particles the surface of each of which is nonporous and inorganic with said reaction enabling means being connected with said fluid inlet means for receiving said compound and reacting material therefrom in liquid form with the mixture thereof being caused to pass through said chamber of said reaction enabling means in a continuous flow whereby said mixture is brought into containing contact with the surface of said particles while in said chamber and whereby reaction of said enzyme with said reacting material continues for a predetermined period of time, said reaction enabling means providing a reaction product output after said predetermined period of time with said reaction product output having a characteristic indicative of detected enzyme; and monitoring means connected with said reaction enabling means to receive said output therefrom, said monitoring means responsive to said characteristic in said output providing an indication of said detected enzyme.
Claim 2
Dependent Claim
203 words
A detection apparatus, comprising: mixing means for receiving and mixing, in liquid form, a compound having at least one enzyme to be detected and reacting material for said enzyme; fluid inlet means for receiving, in liquid form, said mixed compound and reacting material from said mixing means; reaction enabling means including a chamber substantially filled with particles the surface of each of which is nonporous and inorganic with said reaction enabling means being connected with said fluid inlet means for receiving said compound and reacting material therefrom in liquid form with the mixture thereof being caused to pass through said chamber of said reaction enabling means in a continuous flow whereby said mixture is brought into containing contact with the surface of said particles while in said chamber and whereby reaction of said enzyme with said reacting material continues for a predetermined period of time, said reaction enabling means providing a reaction product output after said predetermined period of time with said reaction product output having a characteristic indicative of detected enzyme; and monitoring means connected with said reaction enabling means to receive said output therefrom, said monitoring means responsive to said characteristic in said output providing an indication of said detected enzyme.
Claim 3
Dependent Claim
26 words
The apparatus of claim 1 wherein said particles having non-porous and inorganic surfaces are beads of a size between about 5 and 400 millimicrons nominal diameter.
Claim 4
Dependent Claim
14 words
The apparatus of claim 2 wherein said beads are nonporous inorganic sodasilicate glass beads.
Claim 5
Dependent Claim
25 words
The apparatus of claim 1 wherein said particles having nonporous and inorganic surfaces are beads of a size between 37 and 74 millimicrons nominal diameter.
Claim 6
Dependent Claim
18 words
The apparatus of claim 1 wherein said apparatus forms a part of a high performance liquid chromatography system.
Claim 7
Dependent Claim
206 words
A detection apparatus, comprising: separation means for receiving a compound and separating a plurality of enzymes therein to be detected; fluid inlet means for receiving, in liquid form, from said separation means, said compound having said separated plurality of enzymes therein to be detected and reacting material for said enzymes; reaction enabling means including a chamber substantially filled with particles the surface of each of which is nonporous and inorganic with said reaction enabling means being connected with said fluid inlet means for receiving said compound and reacting material therefrom in liquid form with the mixture thereof being caused to pass through said chamber of said reaction enabling means in a continuous flow whereby said mixture is brought into continuing contact with the surface of said particles while in said chamber and whereby reaction of said enzymes with said reacting material continues for a predetermined period of time, said reaction enabling means providing a reaction product output after said predetermined period of time with said reaction product output have a characteristic indicative of detective enzymes; and monitoring means connected with said reaction enabling means to receive said output therefrom, said monitoring means responsive to said characteristic in said output providing an indication of said detected enzymes.
Claim 8
Dependent Claim
206 words
A detection apparatus, comprising: separation means for receiving a compound and separating a plurality of enzymes therein to be detected; fluid inlet means for receiving, in liquid form, from said separation means, said compound having said separated plurality of enzymes therein to be detected and reacting material for said enzymes; reaction enabling means including a chamber substantially filled with particles the surface of each of which is nonporous and inorganic with said reaction enabling means being connected with said fluid inlet means for receiving said compound and reacting material therefrom in liquid form with the mixture thereof being caused to pass through said chamber of said reaction enabling means in a continuous flow whereby said mixture is brought into continuing contact with the surface of said particles while in said chamber and whereby reaction of said enzymes with said reacting material continues for a predetermined period of time, said reaction enabling means providing a reaction product output after said predetermined period of time with said reaction product output have a characteristic indicative of detective enzymes; and monitoring means connected with said reaction enabling means to receive said output therefrom, said monitoring means responsive to said characteristic in said output providing an indication of said detected enzymes.
Claim 9
Dependent Claim
202 words
A detection apparatus, comprising: fluid inlet means for receiving, in liquid form, a compound having at least one enzyme to be detected and reacting material for said enzyme; reaction enabling means including a chamber substantially filled with particles the surface of each of which is nonporous and inorganic beads of a size between about 5 and 400 millimicrons nominal diameter having a glycidoxypropl trimethoxysilane coating thereon, with said reaction enabling means being connected with said fluid inlet means for receiving said compound and reacting material therefrom in liquid form with the mixture thereof being caused to pass through said chamber of said reaction enabling means in a continuous flow whereby said mixture is brought into continuing contact with the surface of said particles while in said chamber and whereby reaction of said enzymes with said reacting material continues for a predetermined period of time, said reaction enabling means providing a reaction product output after said predetermined period of time with said reaction product output having a characteristic indicative of detected enzymes; and monitoring means connected with said reaction enabling means to receive said output therefrom, said monitoring means responsive to said characteristic in said output providing an indication of said detected enzymes.
Claim 10
Dependent Claim
202 words
A detection apparatus, comprising: fluid inlet means for receiving, in liquid form, a compound having at least one enzyme to be detected and reacting material for said enzyme; reaction enabling means including a chamber substantially filled with particles the surface of each of which is nonporous and inorganic beads of a size between about 5 and 400 millimicrons nominal diameter having a glycidoxypropl trimethoxysilane coating thereon, with said reaction enabling means being connected with said fluid inlet means for receiving said compound and reacting material therefrom in liquid form with the mixture thereof being caused to pass through said chamber of said reaction enabling means in a continuous flow whereby said mixture is brought into continuing contact with the surface of said particles while in said chamber and whereby reaction of said enzymes with said reacting material continues for a predetermined period of time, said reaction enabling means providing a reaction product output after said predetermined period of time with said reaction product output having a characteristic indicative of detected enzymes; and monitoring means connected with said reaction enabling means to receive said output therefrom, said monitoring means responsive to said characteristic in said output providing an indication of said detected enzymes.
Claim 11
Independent Claim
255 words
An apparatus for detecting enzymes in a mixture during continuous flow of said mixture through said apparatus, said apparatus comprising: separating means for separating enzymes in a liquid solution and supplying the same under pressure from said separating means; substrate supplying means for supplying a substrate capable of reacting with said separated enzymes, said substrate being in liquid form and supplied under pressure from the output of said substrate supplying means; mixing means including a tee one input of which is connected to the output of said separating means to receive said liquid solution under pressure therefrom and the other input of which is connected to the output of said substrate supplying means to receive said substrate under pressure therefrom whereby mixing occurs at said tee so that said mixing means provides an output in liquid form that is a mixture of said received liquid solution and substrate; a reaction chamber substantially filled with particles the surface of each of which is nonporous and inorganic, said chamber being connected with said mixing means and receiving said output in liquid form therefrom for passage through said chamber and into contact with the surface of said particles, said substrate reacting with said enzymes during passage through said reaction chamber so that the reaction product output from said chamber has a characteristic indicative of detected enzymes; and monitoring means connected with said reaction chamber to receive the reaction product output therefrom, said monitoring means respective to said characteristic in said output providing an indication of said detected enzymes.
Claim 12
Independent Claim
255 words
An apparatus for detecting enzymes in a mixture during continuous flow of said mixture through said apparatus, said apparatus comprising: separating means for separating enzymes in a liquid solution and supplying the same under pressure from said separating means; substrate supplying means for supplying a substrate capable of reacting with said separated enzymes, said substrate being in liquid form and supplied under pressure from the output of said substrate supplying means; mixing means including a tee one input of which is connected to the output of said separating means to receive said liquid solution under pressure therefrom and the other input of which is connected to the output of said substrate supplying means to receive said substrate under pressure therefrom whereby mixing occurs at said tee so that said mixing means provides an output in liquid form that is a mixture of said received liquid solution and substrate; a reaction chamber substantially filled with particles the surface of each of which is nonporous and inorganic, said chamber being connected with said mixing means and receiving said output in liquid form therefrom for passage through said chamber and into contact with the surface of said particles, said substrate reacting with said enzymes during passage through said reaction chamber so that the reaction product output from said chamber has a characteristic indicative of detected enzymes; and monitoring means connected with said reaction chamber to receive the reaction product output therefrom, said monitoring means respective to said characteristic in said output providing an indication of said detected enzymes.
Claim 13
Dependent Claim
40 words
The apparatus of claim 8 wherein said particles within said reaction chamber are glass beads of between about 5 and 400 millimicrons nominal diameter and whereby the pressure drop through said chamber is between about fifty and several thousand pounds.
Claim 14
Dependent Claim
89 words
For an enzyme detection apparatus, a reaction device comprising a chamber substantially filled with particles the surface of each of which is nonporous and inorganic glass beads having a glycidoxypropl trimethoxysilane coating thereon and having a range of between about 37 and 74 millimicrons nominal diameter whereby separated enzymes may be reacted with substrate during passage through said chamber and into contact with the surface of said particles without appreciable band spreading of the chromatographic profile or demixing of enzymes and substrate during passage through an enzyme detection apparatus.
Claim 15
Dependent Claim
89 words
For an enzyme detection apparatus, a reaction device comprising a chamber substantially filled with particles the surface of each of which is nonporous and inorganic glass beads having a glycidoxypropl trimethoxysilane coating thereon and having a range of between about 37 and 74 millimicrons nominal diameter whereby separated enzymes may be reacted with substrate during passage through said chamber and into contact with the surface of said particles without appreciable band spreading of the chromatographic profile or demixing of enzymes and substrate during passage through an enzyme detection apparatus.
Claim 16
Dependent Claim
23 words
The device of claim 10 wherein said particles are glass beads having a range of between about 5 and 400 millimicrons nominal diameter.
Claim 17
Dependent Claim
215 words
A flow-through system for detecting enzymes in a liquid solution, said system comprising: separation means for separating enzymes in a received liquid solution and providing an output in liquid form with said enzymes separated therein; substrate supply means for supplying substrate capable of reacting with said separated enzymes; mixing means separately connected with said separation means and said substrate supply means to receive the outputs in liquid form therefrom and providing an output in liquid form that is a mixture thereof; a reaction chamber substantially filled with particles the surface of which is non-porous and inorganic, said chamber being connected with said mixing means and receiving said output therefrom in liquid form for passage through said chamber and into contact with the surface of said particles, said substrate reacting with said enzymes during passage through said reaction chamber so that the reaction product output from said chamber has a color characteristic indicative of detected enzymes; a spectrophotometer connected with said reaction chamber to receive the reaction product output therefrom, said spectrophotometer responsive to said color characteristic in said reaction product output providing an indication of said detected enzymes; and pump means connected with said separation means and said substrate supply means whereby a continuous flow is established through said system during detection of said enzymes.
Claim 18
Dependent Claim
215 words
A flow-through system for detecting enzymes in a liquid solution, said system comprising: separation means for separating enzymes in a received liquid solution and providing an output in liquid form with said enzymes separated therein; substrate supply means for supplying substrate capable of reacting with said separated enzymes; mixing means separately connected with said separation means and said substrate supply means to receive the outputs in liquid form therefrom and providing an output in liquid form that is a mixture thereof; a reaction chamber substantially filled with particles the surface of which is non-porous and inorganic, said chamber being connected with said mixing means and receiving said output therefrom in liquid form for passage through said chamber and into contact with the surface of said particles, said substrate reacting with said enzymes during passage through said reaction chamber so that the reaction product output from said chamber has a color characteristic indicative of detected enzymes; a spectrophotometer connected with said reaction chamber to receive the reaction product output therefrom, said spectrophotometer responsive to said color characteristic in said reaction product output providing an indication of said detected enzymes; and pump means connected with said separation means and said substrate supply means whereby a continuous flow is established through said system during detection of said enzymes.
Claim 19
Dependent Claim
69 words
The system of claim 12 wherein said pump means pumps buffer solution to said separation means, wherein said system includes means for permitting injection of a sample containing enzymes into said buffer prior to receipt of said liquid solution at said separation means, and wherein said system includes a pen recorder connected with said spectrophotometer to record enzymes injected into said solution and detected during passage through said system.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-01-06 | 1984-07-07 | 1985-01-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-01-06 | 1988-07-07 | 1989-01-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-01-06 | 1992-07-07 | 1993-01-06 | $7700.00 | Window Closed / Lapsed |