US4241184A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Secondary alcohol dehydrogenase enzyme and use thereof
Assignee: ExxonMobil Technology and Engineering Co
•
Filing: 1979-03-27
•
Granted: 1980-12-23
•
Expires: 1999-03-27
Abstract
Abstract Disclosed is a newly-discovered and isolated enzyme which is a nicotinamide adenine dinucleotide (NAD+)-linked C3-C6 secondary alcohol-specific dehydrogenase. This enzyme, in the presence of oxygen and NAD, is capable of converting C3-C6 secondary alcohols to the corresponding methyl ketones. The purified enzyme has a molecular weight of 95,000 +/- 3,000 dalton as determined by acrylamide gel electrophoresis and contains two zinc atoms per molecule of protein. Primary alcohols are not oxidized by this enzyme.
Inventors
- 👤 Ching-Tsang Hou
- 👤 Ramesh N. Patel
- 👤 Allen I. Laskin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 11:15
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; C12N1/00; C12N1/26; C12N1/28; C12N1/30; C12P; C12P17/00; C12P17/02; C12P7/00; C12P7/02; C12P7/04; C12P7/06; C12P7/24; C12P7/26; C12P7/28; Y; Y02; Y02E; Y02E50/00; Y02E50/10; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/858
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
265 words
A process for converting a C 3 -C 6 secondary alcohol to the corresponding methyl ketone comprising oxidizing said alcohol by contacting said alcohol in a medium, under aerobic conditions, with microbial cells derived from a methylotrophic microorganism or an enzyme preparation derived from said cells, wherein said microorganism has been previously grown under aerobic conditions in a nutrient medium containing a carbon-containing compound which provides the carbon and energy source for growth of the cells and induces C 3 -C 6 secondary alcohol dehydrogenase enzyme activity in said cells, and wherein said microorganism is selected from the group consisting of: ______________________________________
Methylosinus trichosporium
(CRL 15 PM1)
NRRL B-11,202;
Methylosinus sporium
(CRL 16 PM2)
NRRL B-11,203;
Methylocystis parvus
(CRL 18 PM4)
NRRL B-11,204;
Methylomonas methanica
(CRL M4P) NRRL B-11,205;
Methylomonas methanica
(CRL 21 PM7)
NRRL B-11,206;
Methylomonas albus
(CRL M8Y) NRRL B-11,207;
Methylomonas
streptobacterium
(CRL 17 PM3)
NRRL B-11,208;
Methylomonas agile
(CRL 22 PM9)
NRRL B-11,209;
Methylomonas rubrum
(CRL M6P) NRRL B-11,210;
Methylomonas rubrum
(CRL 20 PM6)
NRRL B-11,211;
Methylomonas rosaceus
(CRL M10P) NRRL B-11,212;
Methylomonas rosaceus
(CRL M7P) NRRL B-11,213;
Methylobacter chroococcum
(CRL M6) NRRL B-11,214;
Methylobacter chroococcum
(CRL 23 PM8)
NRRL B-11,215;
Methylobacter bovis
(CRL M1Y) NRRL B-11,216;
Methylobacter bovis
(CRL 19 PM5)
NRRL B-11,217;
Methylobacter vinelandii
(CRL M5Y) NRRL B-11,218;
Methylococcus capsulatus
(CRL M1) NRRL B-11,219;
Methylococcus minimus
(CRL 24 PM12)
NRRL B-11,220;
Methylococcus capsulatus
(CRL 25 PM13)
NRRL B-11,221;
Methylobacterium
(CRL 26 R6) NRRL B-11,222;
organophilum
Pichia sp. (CRL-72) NRRL-Y-11,328;
Torulopsis sp. (A.sub.1) NRRL-Y-11,419;
Kloeckera sp. (A.sub.2) NRRL-Y-11,420;
______________________________________ and mutants thereof wherein said methyl ketone is produced in isolable amounts.
Claim 2
Dependent Claim
265 words
A process for converting a C 3 -C 6 secondary alcohol to the corresponding methyl ketone comprising oxidizing said alcohol by contacting said alcohol in a medium, under aerobic conditions, with microbial cells derived from a methylotrophic microorganism or an enzyme preparation derived from said cells, wherein said microorganism has been previously grown under aerobic conditions in a nutrient medium containing a carbon-containing compound which provides the carbon and energy source for growth of the cells and induces C 3 -C 6 secondary alcohol dehydrogenase enzyme activity in said cells, and wherein said microorganism is selected from the group consisting of: ______________________________________
Methylosinus trichosporium
(CRL 15 PM1)
NRRL B-11,202;
Methylosinus sporium
(CRL 16 PM2)
NRRL B-11,203;
Methylocystis parvus
(CRL 18 PM4)
NRRL B-11,204;
Methylomonas methanica
(CRL M4P) NRRL B-11,205;
Methylomonas methanica
(CRL 21 PM7)
NRRL B-11,206;
Methylomonas albus
(CRL M8Y) NRRL B-11,207;
Methylomonas
streptobacterium
(CRL 17 PM3)
NRRL B-11,208;
Methylomonas agile
(CRL 22 PM9)
NRRL B-11,209;
Methylomonas rubrum
(CRL M6P) NRRL B-11,210;
Methylomonas rubrum
(CRL 20 PM6)
NRRL B-11,211;
Methylomonas rosaceus
(CRL M10P) NRRL B-11,212;
Methylomonas rosaceus
(CRL M7P) NRRL B-11,213;
Methylobacter chroococcum
(CRL M6) NRRL B-11,214;
Methylobacter chroococcum
(CRL 23 PM8)
NRRL B-11,215;
Methylobacter bovis
(CRL M1Y) NRRL B-11,216;
Methylobacter bovis
(CRL 19 PM5)
NRRL B-11,217;
Methylobacter vinelandii
(CRL M5Y) NRRL B-11,218;
Methylococcus capsulatus
(CRL M1) NRRL B-11,219;
Methylococcus minimus
(CRL 24 PM12)
NRRL B-11,220;
Methylococcus capsulatus
(CRL 25 PM13)
NRRL B-11,221;
Methylobacterium
(CRL 26 R6) NRRL B-11,222;
organophilum
Pichia sp. (CRL-72) NRRL-Y-11,328;
Torulopsis sp. (A.sub.1) NRRL-Y-11,419;
Kloeckera sp. (A.sub.2) NRRL-Y-11,420;
______________________________________ and mutants thereof wherein said methyl ketone is produced in isolable amounts.
Claim 3
Dependent Claim
26 words
The process of claim 1 wherein the enzyme preparation is a bacterial or yeast cell suspension which has been grown on a methyl radical donating compound.
Claim 4
Dependent Claim
12 words
The process of claim 2 wherein said enzyme preparation comprises microbial cells.
Claim 5
Dependent Claim
19 words
The process of claim 1 wherein said enzyme preparation is cell-free and the medium additionally contains nicotinamide adenine dinucleotide.
Claim 6
Dependent Claim
16 words
The process of claim 4 wherein the enzyme preparation is a bacterial- or yeast-derived cell-free extract.
Claim 7
Dependent Claim
19 words
The process of claim 4 wherein the enzyme preparation is substantially purified C 3 -C 6 secondary alcohol dehydrogenase.
Claim 8
Dependent Claim
35 words
The process of claim 4 wherein the conversion is carried out at a temperature ranging from about 5° to about 55° C. and at a pH in the range from about 4 to about 9.
Claim 9
Dependent Claim
10 words
The process of claim 4 wherein the enzyme is immobilized.
Claim 10
Dependent Claim
12 words
The process of claim 4 wherein the conversion is carried out batchwise.
Claim 11
Dependent Claim
20 words
The process of claim 4 wherein the conversion is carried out in a continuous manner and said enzyme is immobilized.
Claim 12
Dependent Claim
36 words
The process of claim 4 wherein the enzyme, C 3 -C 6 secondary alcohol dehydrogenase, has a molecular weight of 95,000±3,000 dalton as determined by Bio-gel column chromatography and two zinc atoms per molecule of protein.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-12-23 | 1984-06-23 | 1984-12-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-12-23 | 1988-06-23 | 1988-12-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-12-23 | 1992-06-23 | 1992-12-23 | $7700.00 | Window Closed / Lapsed |