US4237033A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Pretreatment of microcarriers for cell culture
Assignee: Merck and Co Inc
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Filing: 1979-04-23
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Granted: 1980-12-02
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Expires: 1999-04-23
Abstract
Abstract A method is provided for pretreatment of microcarrier beads to make them suitable for use in mass culture of vertebrate anchorage dependent cells such as primary and diploid cells. The beads are soaked and heated in fetal calf serum (FCS) at from about 65 DEG to about 95 DEG C. for about 3 minutes to about 1 hour.
Inventors
- 👤 Edgar M. Scattergood
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 23:36
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N5/0075; C12N2533/00; C12N2533/70
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
33 words
A process of treating microcarrier beads to enhance their suitability as a surface for cell culture which comprises soaking the beads in heated fetal calf serum prior to culturing cells on the beads.
Claim 2
Dependent Claim
33 words
A process of treating microcarrier beads to enhance their suitability as a surface for cell culture which comprises soaking the beads in heated fetal calf serum prior to culturing cells on the beads.
Claim 3
Dependent Claim
24 words
A process according to claim 1 wherein the soaking is carried out for a period of from about 3 minutes to about 1 hour.
Claim 4
Dependent Claim
24 words
A process according to claim 2 wherein the soaking is carried out for a period of from about 5 minutes to about 20 minutes.
Claim 5
Dependent Claim
23 words
A process according to claim 1 wherein the heated fetal calf serum has a temperature of from about 65° to about 95° C.
Claim 6
Dependent Claim
23 words
A process according to claim 4 wherein the heated fetal calf serum has a temperature of from about 75° to about 90° C.
Claim 7
Dependent Claim
9 words
The beads prepared by the process of claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-12-02 | 1984-06-02 | 1984-12-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-12-02 | 1988-06-02 | 1988-12-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-12-02 | 1992-06-02 | 1992-12-02 | $7700.00 | Window Closed / Lapsed |