US4235969A
Expired / Lapsed
US
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Procainamide antigen conjugates and antibodies
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1978-05-08
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Granted: 1980-11-25
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Expires: 1998-05-08
Abstract
Abstract Compounds are provided for use in the preparation of reagents which can be used in immunoassays for the determination of benzamides of N,N-dialkylethyleneamines. A linking group is provided, at a particular site of the drug, which provides a link between the above compounds and an antigen, with the resulting conjugate being employed for the preparation of antibodies. The antibodies find particular use in competitive protein binding assays. Conjugates to enzymes are prepared which find particular use in homogeneous enzyme immunoassays.
Inventors
- 👤 Prithipal Singh
- 👤 Marcel R. Pirio
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 20:52
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S435/964; Y10S436/00; Y10S436/815; Y10S436/816; Y10S436/823; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
116 words
A compound of the formula: ##STR4## wherein: Z is hydrogen or acetyl; Y is alkyl of from 1 to 3 carbon atoms; X is oxygen or imino (NH); R is a linking of group of from 1 to 8 carbon atoms having from 0 to 4 heteroatoms, which are oxygen, nitrogen and sulfur, wherein the oxygen is present as oxy or nonoxocarbonyl, nitrogen is present as tertiary amino, amido or imino and sulfur is present as thioether or thiono, and having from 0 to 1 site of ethylenic unsaturation as the only unsaturation; A is a poly(amino acid) which is antigenic or an enzyme; n is 1 to the molecular weight of A divided by 1000.
Claim 2
Dependent Claim
116 words
A compound of the formula: ##STR4## wherein: Z is hydrogen or acetyl; Y is alkyl of from 1 to 3 carbon atoms; X is oxygen or imino (NH); R is a linking of group of from 1 to 8 carbon atoms having from 0 to 4 heteroatoms, which are oxygen, nitrogen and sulfur, wherein the oxygen is present as oxy or nonoxocarbonyl, nitrogen is present as tertiary amino, amido or imino and sulfur is present as thioether or thiono, and having from 0 to 1 site of ethylenic unsaturation as the only unsaturation; A is a poly(amino acid) which is antigenic or an enzyme; n is 1 to the molecular weight of A divided by 1000.
Claim 3
Dependent Claim
15 words
A compound according to claim 1 wherein A is a poly(amino acid) which is antigenic.
Claim 4
Dependent Claim
22 words
A compound according to claim 1 wherein A is a poly(amino acid) which is an enzyme, and n is 1 to 30.
Claim 5
Dependent Claim
15 words
A compound according to claim 3 wherein said enzyme is an oxidoreductase or a hydrolase.
Claim 6
Dependent Claim
12 words
A compound according to claim 3 wherein said enzyme is glucose-6-phosphate dehydrogenase.
Claim 7
Dependent Claim
50 words
A compound of the formula: ##STR5## wherein: Z 1 is hydrogen or acetyl; R 1 is alkylene of from 1 to 4 carbon atoms; A 1 is a poly(amino acid) which is antigenic or an enzyme; n 1 is 1 to the molecular weight of A 1 divided by 1000.
Claim 8
Dependent Claim
50 words
A compound of the formula: ##STR5## wherein: Z 1 is hydrogen or acetyl; R 1 is alkylene of from 1 to 4 carbon atoms; A 1 is a poly(amino acid) which is antigenic or an enzyme; n 1 is 1 to the molecular weight of A 1 divided by 1000.
Claim 9
Dependent Claim
16 words
A compound according to claim 6 wherein A 1 is a poly(amino acid) which is antigenic.
Claim 10
Dependent Claim
24 words
A compound according to claim 6 wherein A 1 is an enzyme, said enzyme being glucose-6-phosphate dehydrogenase, and n 1 is 1 to 30.
Claim 11
Dependent Claim
37 words
A compound of the formula: ##STR6## wherein: Z 2 is hydrogen or acetyl; A 2 is bovine serum albumin or bovine gamma globulin; n 2 is 1 to the molecular weight of A 2 divided by 1000.
Claim 12
Dependent Claim
37 words
A compound of the formula: ##STR6## wherein: Z 2 is hydrogen or acetyl; A 2 is bovine serum albumin or bovine gamma globulin; n 2 is 1 to the molecular weight of A 2 divided by 1000.
Claim 13
Dependent Claim
24 words
A compound of the formula: ##STR7## wherein: Z 3 is hydrogen or acetyl; A 3 is glucose-6-phosphate dehydrogenase; n 3 is 1 to 30.
Claim 14
Dependent Claim
24 words
A compound of the formula: ##STR7## wherein: Z 3 is hydrogen or acetyl; A 3 is glucose-6-phosphate dehydrogenase; n 3 is 1 to 30.
Claim 15
Dependent Claim
67 words
An antibody composition prepared in immunological response to procainamide or N-acetyl procainamide conjugated to an antigen through the amino group of the aminoalkyl bonded to the nitrogen of the benzamide group of procainamide, said antibody being capable of binding to procainamide and an enzyme conjugate according to claim 4, wherein Z is hydrogen, or N-acetylprocainamide and an enzyme conjugate according to claim 4, wherein Z is acetyl.
Claim 16
Dependent Claim
69 words
An antibody composition prepared in immunological response to procainamide or N-acetyl procainamide conjugated to an antigen through the amino group of the aminoalkyl bonded to the nitrogen of the benzamide group of procainamide, and said antibody capable of binding to procainamide and an enzyme conjugate according to claim 10, wherein Z 3 is hydrogen, or N-acetyl-procainamide and an enzyme conjugate according to claim 10, wherein Z 3 is acetyl.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-11-25 | 1984-05-26 | 1984-11-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-11-25 | 1988-05-26 | 1988-11-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-11-25 | 1992-05-26 | 1992-11-25 | $7700.00 | Window Closed / Lapsed |