Abstract Natural heteropolysaccharide, specifically hexuronyl hexosaminoglycane sulfate and, a process to obtain it, which substantially provides for the following steps: (a) hydrolysis of an animal organ with a proteolytic enzyme, in water; (b) precipitation of the hydrolyzed product with a water miscible solvent; (c) solubilization of the precipitate in a solution of a salt of a strong mineral acid; (d) addition of a quaternary ammonium halide to form a soluble complex and a precipitate which is separated; (e) precipitation of such complex by dilution with water; (f) isolation and solubilization of the precipitated complex in a salt solution according to step (c); (g) precipitation of the final product with a water miscible solvent and drying thereof; Such compound is utilized as active principle in pharmaceutical compositions, particularly suitable for the prevention of thrombotic states.
Hexuronyl hexosaminoglycane sulfate, consisting essentially of: ______________________________________
Hexosamines 29 ± 3%
Huronic acids 27 ± 3%
(Organic) SO.sub.4 .sup.=
27 ± 4%
Acetyl groups 7 ± 1%
Sodium 10 ± 2%
______________________________________ such that the molar ratio of huronic acids: hexosamine:sulfate:acetyl:sodium is 1:1.2:2:1.2:3 PM (chromatography by exclusion on gel): 8.000-16.000 Specific rotary power [α].sub.D.sup.20 =-30°/-15° Electrophoresis on cellulose acetate (buffer:pyridine-acetic-acid-water=1:10:229, pH 4.5 and development with toluidine blue): one main band with electrophoretic mobility U=1.90-1.95 ×10 -4 cm 2 v -1 s -1 I.R. spectrum: characteristic bands are observed at 1740, 1647, 1555, 1375, 1235 and 1050 cm -1 . solubility: soluble in water, in diluted mineral acids and diluted fixed alkalies but insoluble in ethanol.
Claim 2
Dependent Claim
115 words
Hexuronyl hexosaminoglycane sulfate, consisting essentially of: ______________________________________
Hexosamines 29 ± 3%
Huronic acids 27 ± 3%
(Organic) SO.sub.4 .sup.=
27 ± 4%
Acetyl groups 7 ± 1%
Sodium 10 ± 2%
______________________________________ such that the molar ratio of huronic acids: hexosamine:sulfate:acetyl:sodium is 1:1.2:2:1.2:3 PM (chromatography by exclusion on gel): 8.000-16.000 Specific rotary power [α].sub.D.sup.20 =-30°/-15° Electrophoresis on cellulose acetate (buffer:pyridine-acetic-acid-water=1:10:229, pH 4.5 and development with toluidine blue): one main band with electrophoretic mobility U=1.90-1.95 ×10 -4 cm 2 v -1 s -1 I.R. spectrum: characteristic bands are observed at 1740, 1647, 1555, 1375, 1235 and 1050 cm -1 . solubility: soluble in water, in diluted mineral acids and diluted fixed alkalies but insoluble in ethanol.
Claim 3
Dependent Claim
22 words
A pharmaceutical composition, comprising a carrier and as the active ingredient the product of claim 1 in the dosage of 100-200 mg/day.