US4229436A
Expired / Lapsed
US
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Antibiotic G-6302
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1978-12-15
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Granted: 1980-10-21
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Expires: 1998-12-15
Abstract
Abstract A novel Antibiotic G-6302 is produced by cultivating a microorganism belonging to the genus Pseudomonas and being capable of producing Antibiotic G-6302 in a culture medium to have Antibiotic G-6302 elaborated and accumulated in the cultured broth and recovering the antibiotic. Antibiotic G-6302 is useful as a germicide or disinfectant.
Inventors
- 👤 Akira Imada
- 👤 Kazuaki Kitano
- 👤 Mitsuko Asai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 00:23
CPC Classifications
C; C12; C12P; C12P1/00; C12P1/04; A; A61; A61P; A61P31/00; A61P31/04; C12N; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/38
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
168 words
A member selected from the group consisting of Antibiotic G-6302 having the following characteristics, and a metal or ammonium salt thereof: (1) Melting point: 120° C. (sintering), 130° C. (decomp.) (2) Appearance: white powder (3) Elemental analysis: (%) C 34.99, ±0.5; H 5.58, ±0.5; N 13.30, ±0.5; O 38.13, ±0.5; S 7.70, ±0.5 (as dried at 40° C. for 6 hours over phosphorus pentoxide under reduced pressure) (4) Ultraviolet absorption spectrum: No characteristic absorptions over 210 nm (5) Infrared absorption spectrum [KBr, dominant absorptions (wave-numbers, cm -1 )]: 1770, 1650, 1530, 1240, 1043 (6) Specific rotation: [α] D 23 +94°±10° (c=0.35, H 2 O) (7) Solubility: Insoluble in petroleum ether, hexane, diethyl ether, benzene, ethyl acetate and chloroform; sparingly soluble in ethanol, pyridine and acetone; soluble in methanol and dimethylsulfoxide; and readily soluble in water (8) Basicity, neutrality or acidity: Acid (9) Molecular weight: 400±20 (by titrometry) (10) Color reactions: Positive: ninhydrin and potassium permanganate reactions; negative: ferric chloride-potassium ferricyanate, Sakaguchi and Molisch reactions; doubtful positive: Ehrlich reaction.
Claim 2
Dependent Claim
168 words
A member selected from the group consisting of Antibiotic G-6302 having the following characteristics, and a metal or ammonium salt thereof: (1) Melting point: 120° C. (sintering), 130° C. (decomp.) (2) Appearance: white powder (3) Elemental analysis: (%) C 34.99, ±0.5; H 5.58, ±0.5; N 13.30, ±0.5; O 38.13, ±0.5; S 7.70, ±0.5 (as dried at 40° C. for 6 hours over phosphorus pentoxide under reduced pressure) (4) Ultraviolet absorption spectrum: No characteristic absorptions over 210 nm (5) Infrared absorption spectrum [KBr, dominant absorptions (wave-numbers, cm -1 )]: 1770, 1650, 1530, 1240, 1043 (6) Specific rotation: [α] D 23 +94°±10° (c=0.35, H 2 O) (7) Solubility: Insoluble in petroleum ether, hexane, diethyl ether, benzene, ethyl acetate and chloroform; sparingly soluble in ethanol, pyridine and acetone; soluble in methanol and dimethylsulfoxide; and readily soluble in water (8) Basicity, neutrality or acidity: Acid (9) Molecular weight: 400±20 (by titrometry) (10) Color reactions: Positive: ninhydrin and potassium permanganate reactions; negative: ferric chloride-potassium ferricyanate, Sakaguchi and Molisch reactions; doubtful positive: Ehrlich reaction.
Claim 3
Dependent Claim
75 words
A method for producing Antibiotic G-6302 as defined in claim 1 comprising cultivating a microorganism having the characteristics of Pseudomonas acidophila G-6302 (ATCC 31363) in a culture medium containing an assimilable carbon source and a digestible nitrogen source under aerobic conditions at pH 4 to 8 and at a temperature of the range of 15° to 35° C. until Antibiotic G-6302 is substantially elaborated and accumulated in the cultured broth and recovering Antibiotic G-6302 therefrom.
Claim 4
Dependent Claim
45 words
A method as claimed in claim 2, wherein the culture medium is supplemented with 0.01 to 1.0 w/v% of a sulfur compound which is assimilated by the microorganism and which is selected from the group consisting of sodium thiosulfate, sodium sulfate, cystine, cysteine and methionine.
References Cited (Backward Citations)
US3629405A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-10-21 | 1984-04-21 | 1984-10-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-10-21 | 1988-04-21 | 1988-10-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-10-21 | 1992-04-21 | 1992-10-21 | $7700.00 | Window Closed / Lapsed |