US4228238A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of laboratory testing in water-based culture media for zones of inhibition
Assignee: Allor Foundation
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Filing: 1979-08-23
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Granted: 1980-10-14
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Expires: 1999-08-23
Abstract
Abstract This disclosure is concerned with providing extractions of oil-based anti-microbial materials, such as essential oil extracts and the like, that can be rendered miscible with water-based culture media with which they are otherwise non-miscible, to enable zone of inhibition testing with such culture media.
Inventors
- 👤 Damon Swanson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 20:56
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/18
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
135 words
A method of enabling zone of inhibition testing of essential oil extracts with non-miscible oil-based culture media and the like, that comprises, mixing said essential oil extracts with an alcohol that, in relatively dilute concentration, does not demonstrate substantial anti-microbial activity, to form an emulsion containing an extraction of the same materials; diluting the alcohol emulsion with water sufficient to bring the alcohol to said relatively dilute concentration of non-anti-microbial activity; saturating a sensitivity disc with a sample of the diluted emulsion after contacting the disc with a water-based culture medium; evaporating the alcohol saturated into the disc; inverting and incubating the culture medium carrying the disc; and observing zones of inhibition produced on the disc as a result of miscible reaction with the water-based culture medium of the alcohol-extracted originally non-miscible essential oil extracts.
Claim 2
Independent Claim
135 words
A method of enabling zone of inhibition testing of essential oil extracts with non-miscible oil-based culture media and the like, that comprises, mixing said essential oil extracts with an alcohol that, in relatively dilute concentration, does not demonstrate substantial anti-microbial activity, to form an emulsion containing an extraction of the same materials; diluting the alcohol emulsion with water sufficient to bring the alcohol to said relatively dilute concentration of non-anti-microbial activity; saturating a sensitivity disc with a sample of the diluted emulsion after contacting the disc with a water-based culture medium; evaporating the alcohol saturated into the disc; inverting and incubating the culture medium carrying the disc; and observing zones of inhibition produced on the disc as a result of miscible reaction with the water-based culture medium of the alcohol-extracted originally non-miscible essential oil extracts.
Claim 3
Dependent Claim
16 words
A method as claimed in claim 1 in which said essential oil extracts are anti-microbial materials.
Claim 4
Dependent Claim
24 words
A method as claimed in claim 1 and in which said alcohol is ethanol and said relatively dilute concentration is less than about 50%.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-10-14 | 1984-04-14 | 1984-10-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-10-14 | 1988-04-14 | 1988-10-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-10-14 | 1992-04-14 | 1992-10-14 | $7700.00 | Window Closed / Lapsed |