US4224409A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for producing L-proline by fermentation
Assignee: Ajinomoto Co Inc
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Filing: 1979-01-30
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Granted: 1980-09-23
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Expires: 1999-01-30
Abstract
Abstract A method for producing L-proline by fermentation which comprises culturing an L-proline producing mutant in a culture medium until L-proline is accumulated in the culture medium, and recovering the accumulated L-proline; said mutant belonging to the genus Brevibacterium, Corynebacterium or Microbacterium and being resistant to DL-3,4-dehydroproline.
Inventors
- 👤 Shigeru Nakamori
- 👤 Hajimu Morioka
- 👤 Fumihiro Yoshinaga
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 19:26
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/24
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
46 words
A method for producing L-proline by fermentation which comprises culturing an L-proline producing mutant in a culture medium until L-proline is accumulated in the culture medium, and recovering the accumulated L-proline; said mutant belonging to the genus Brevibacterium, Corynebacterium or Microbacterium and being resistant to DL-3,4-dehydroproline.
Claim 2
Independent Claim
46 words
A method for producing L-proline by fermentation which comprises culturing an L-proline producing mutant in a culture medium until L-proline is accumulated in the culture medium, and recovering the accumulated L-proline; said mutant belonging to the genus Brevibacterium, Corynebacterium or Microbacterium and being resistant to DL-3,4-dehydroproline.
Claim 3
Dependent Claim
25 words
A method according to claim 1, wherein said mutant is selected from the group consisting of Brevibacterium lactofermentum, Brevibacterium flavum, Corynebacterium glutamicum, and Microbacterium ammoniaphilum.
Claim 4
Dependent Claim
13 words
A method according to claim 1, wherein said mutant requires L-isoleucine for growth.
Claim 5
Independent Claim
63 words
A method for producing L-proline by fermentation which comprises culturing an L-proline producing mutant in a culture medium until L-proline is accumulated in the culture medium, and recovering the accumulated L-proline, wherein said mutant is selected from the class consisting of Brevibacterium lactofermentum, NRRL B-11421; Brevibacterium flavum, NRRL B-11422; Corynebacterium glutamicum, NRRL B-11423; and Microbacterium ammoniaphilum, NRRL B-11424, and being resistant to DL-3,4-dehydroproline.
Claim 6
Independent Claim
63 words
A method for producing L-proline by fermentation which comprises culturing an L-proline producing mutant in a culture medium until L-proline is accumulated in the culture medium, and recovering the accumulated L-proline, wherein said mutant is selected from the class consisting of Brevibacterium lactofermentum, NRRL B-11421; Brevibacterium flavum, NRRL B-11422; Corynebacterium glutamicum, NRRL B-11423; and Microbacterium ammoniaphilum, NRRL B-11424, and being resistant to DL-3,4-dehydroproline.
Claim 7
Dependent Claim
16 words
A process according to claim 4, wherein the L-proline producing mutant is Brevibacterium lactofermentum NRRL B-11421.
Claim 8
Dependent Claim
16 words
A process according to claim 4, wherein the L-proline producing mutant is Brevibacterium flavum NRRL B-11422.
Claim 9
Dependent Claim
16 words
A process according to claim 4, wherein the L-proline producing mutant is Corynebacterium glutamicum NRRL B-11423.
Claim 10
Dependent Claim
16 words
A process according to claim 4, wherein the L-proline producing mutant is Microbacterium ammoniaphilum NRRL B-11424.
Claim 11
Dependent Claim
13 words
A process according to claim 4, wherein said mutant requires L-isoleucine for growth.
References Cited (Backward Citations)
US3819483A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-09-23 | 1984-03-24 | 1984-09-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-09-23 | 1988-03-24 | 1988-09-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-09-23 | 1992-03-24 | 1992-09-23 | $7700.00 | Window Closed / Lapsed |