US4224404A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of specific immune nucleic acids cell dialysates and antibodies
Assignee: International Institute of Differentiation Ltd
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Filing: 1977-12-14
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Granted: 1980-09-23
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Expires: 1997-12-14
Abstract
Abstract Gamma-globulins and immune nucleic acids, both specific to a given antigen, are produced by culturing in vitro an inducible cell line in the presence of specific immune nucleic acids or specific immune dialysates obtained either from human or animal donor lymphoid cells sensitized to said specific antigen or from cells of a previously induced cell line sensitized to said specific antigen, or in the presence of the liquid phase of a culture of a previously induced cell line sensitized to said specific antigen; and extracting gamma-globulins from the liquid phase of the thus induced cell culture and/or extracting the immune nucleic acids from the cells so cultured. Specific immune dialysates may also be produced by a similar process.
Inventors
- 👤 Dimitri Viza
- 👤 Dimitri Adamopoulos
- 👤 John Phillips
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 21:23
CPC Classifications
C; C07; C07H; C07H21/00; C07K; C07K16/00
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
192 words
A process for the production of gamma-globulins specific to a given antigen, which process comprises culturing an inducible established cell line of lymphoid origin, which spontaneously produces gamma-globulins, in vitro in the presence of material which is specific to said given antigen and which is selected from: (a) immune nucleic acids obtained from the lymphoid cells of donors sensitised to said given antigen, said donors being selected from humans and animals, (b) immune nucleic acids obtained from cells of an established cell line culture sensitised to said given antigen by having been cultured with immune nucleic acids or immune dialysates specific to said given antigen, (c) immune dialysates obtained from the lymphoid cells of donors sensitised to said given antigen, said donors being selected from humans and animals, (d) immune dialysates obtained from cells of an established cell line culture sensitised to said given antigen by having been cultured with immune nucleic acids or immune dialysates specific to said given antigen, or (e) the liquid phase of an established cell line culture sensitised to said given antigen by having been cultured with immune nucleic acids or immune dialysates specific to said antigen.
Claim 2
Dependent Claim
192 words
A process for the production of gamma-globulins specific to a given antigen, which process comprises culturing an inducible established cell line of lymphoid origin, which spontaneously produces gamma-globulins, in vitro in the presence of material which is specific to said given antigen and which is selected from: (a) immune nucleic acids obtained from the lymphoid cells of donors sensitised to said given antigen, said donors being selected from humans and animals, (b) immune nucleic acids obtained from cells of an established cell line culture sensitised to said given antigen by having been cultured with immune nucleic acids or immune dialysates specific to said given antigen, (c) immune dialysates obtained from the lymphoid cells of donors sensitised to said given antigen, said donors being selected from humans and animals, (d) immune dialysates obtained from cells of an established cell line culture sensitised to said given antigen by having been cultured with immune nucleic acids or immune dialysates specific to said given antigen, or (e) the liquid phase of an established cell line culture sensitised to said given antigen by having been cultured with immune nucleic acids or immune dialysates specific to said antigen.
Claim 3
Dependent Claim
17 words
A process according to claim 1 wherein said inducible established cell line is a myeloma cell line.
Claim 4
Dependent Claim
17 words
A process according to claim 1 wherein said inducible established cell line is a lymphoblastoid cell line.
Claim 5
Dependent Claim
33 words
A process according to claim 1 wherein said inducible established cell line is selected from the group consisting of the LDV/7 cell line, the BRI 8 cell line and the BEC/11 cell line.
Claim 6
Dependent Claim
22 words
A process according to claim 1 wherein said material (a) is i-RNA obtained from the lymphoid cells of a sensitised animal donor.
Claim 7
Dependent Claim
19 words
A process according to claim 5 wherein the animal is selected from a sheep, rabbit, guinea pig and rat.
Claim 8
Dependent Claim
25 words
A process according to claim 1 wherein said material (b) is i-RNA obtained from the cells of a LDV/7 culture sensitised to said given antigen.
Claim 9
Dependent Claim
23 words
A process according to claim 1 wherein said material (d) is obtained from cells of a LDV/7 culture sensitised to said given antigen.
Claim 10
Dependent Claim
25 words
A process according to claim 1 wherein said given antigen is selected from Keyhole-limpet haemocyanin, coccidioidin, histoplasmin, brucella bacteria, melanoma cells and colon carcinoma cells.
Claim 11
Dependent Claim
28 words
A process according to claim 1 which further comprises cloning the resulting established cell line of lymphoid origin which is spontaneously producing gamma-globulins specific to said given antigen.
Claim 12
Dependent Claim
211 words
A process for the production of immune nucleic acids and immune dialysates, both specific to a given antigen, which process comprises culturing an inducible established cell line of lymphoid origin, which spontaneously produces gamma-globulins, in vitro in the presence of material which is specific to said antigen and which is selected from: (a) immune nucleic acids obtained from the lymphoid cells of donors sensitised to said given antigen, said donors being selected from humans and animals, (b) immune nucleic acids obtained from cells of an established cell line culture sensitised to said given antigen by having been cultured with immune nucleic acids or immune dialysates specific to said given antigen, or (c) the liquid phase of an established cell line culture sensitised to said given antigen by having been cultured with immune nucleic acids or immune dialysates specific to said antigen, or in the case of the production of immune nucleic acids, also from: (d) immune dialysates obtained from the lymphoid cells of donors sensitised to said given antigen, said donors being selected from humans and animals, or (e) immune dialysates obtained from cells of an established cell line culture sensitised to said given antigen by having been cultured with immune nucleic acids or immune dialysates specific to said given antigen.
Claim 13
Dependent Claim
211 words
A process for the production of immune nucleic acids and immune dialysates, both specific to a given antigen, which process comprises culturing an inducible established cell line of lymphoid origin, which spontaneously produces gamma-globulins, in vitro in the presence of material which is specific to said antigen and which is selected from: (a) immune nucleic acids obtained from the lymphoid cells of donors sensitised to said given antigen, said donors being selected from humans and animals, (b) immune nucleic acids obtained from cells of an established cell line culture sensitised to said given antigen by having been cultured with immune nucleic acids or immune dialysates specific to said given antigen, or (c) the liquid phase of an established cell line culture sensitised to said given antigen by having been cultured with immune nucleic acids or immune dialysates specific to said antigen, or in the case of the production of immune nucleic acids, also from: (d) immune dialysates obtained from the lymphoid cells of donors sensitised to said given antigen, said donors being selected from humans and animals, or (e) immune dialysates obtained from cells of an established cell line culture sensitised to said given antigen by having been cultured with immune nucleic acids or immune dialysates specific to said given antigen.
Claim 14
Dependent Claim
17 words
A process according to claim 11 wherein said inducible established cell line is a myeloma cell line.
Claim 15
Dependent Claim
17 words
A process according to claim 11 wherein said inducible established cell line is a lymphoblastoid cell line.
Claim 16
Dependent Claim
32 words
A process according to claim 11 wherein said inducible established cell line is selected from the group consisting of the LDV/7 cell line, the BRI/8 cell line and the BEC/11 cell line.
Claim 17
Dependent Claim
22 words
A process according to claim 11 wherein said material (a) is i-RNA obtained from the lymphoid cells of a sensitised animal donor.
Claim 18
Dependent Claim
19 words
A process according to claim 15 wherein the animal is selected from a sheep, rabbit, guinea pig and rat.
Claim 19
Dependent Claim
25 words
A process according to claim 11 wherein said material (b) is i-RNA obtained from the cells of a LDV/7 culture sensitised to said given antigen.
Claim 20
Dependent Claim
23 words
A process according to claim 11 wherein said material (e) is obtained from cells of a LDV/7 culture sensitised to said given antigen.
Claim 21
Dependent Claim
25 words
A process according to claim 11 wherein said given antigen is selected from Keyhole-limpet haemocyanin, coccidioidin, histoplasmin, brucella bacteria, melanoma cells and colon carcinoma cells.
References Cited (Backward Citations)
GB1229888A
Cited
GB1443948A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-09-23 | 1984-03-24 | 1984-09-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-09-23 | 1988-03-24 | 1988-09-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-09-23 | 1992-03-24 | 1992-09-23 | $7700.00 | Window Closed / Lapsed |