US4223013A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Amitriptyline conjugates to antigenic proteins and enzymes
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1978-12-29
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Granted: 1980-09-16
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Expires: 1998-12-29
Abstract
Abstract Amitriptyline functionalized compounds are provided for conjugation to antigenic compositions, particularly poly(amino acids), and enzymes. The antigenic conjugates are employed for the production of antibodies, which find particular use in immunoassays for the determination of amitriptyline, while the enzyme conjugate finds use in a homogeneous enzyme immunoassay for the determination of amitriptyline.
Inventors
- 👤 Mae W. Hu
- 👤 Prithipal Singh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 14:50
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/44; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/823; Y10S530/00; Y10S530/806; Y10S530/81; Y10S530/812
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
63 words
Compound of the formula; (Ami--X--RCO--).sub.n Z wherein: Ami is of the formula: ##STR3## X is methylene or carbonyl; Z is a poly(amino acid): n is in the range of one to the molecular weight of Z divided by 500; and R is an aliphatic linking group of from about 1 to 7 carbon atoms having from 0 to 1 site of aliphatic unsaturation.
Claim 2
Dependent Claim
63 words
Compound of the formula; (Ami--X--RCO--).sub.n Z wherein: Ami is of the formula: ##STR3## X is methylene or carbonyl; Z is a poly(amino acid): n is in the range of one to the molecular weight of Z divided by 500; and R is an aliphatic linking group of from about 1 to 7 carbon atoms having from 0 to 1 site of aliphatic unsaturation.
Claim 3
Dependent Claim
21 words
Compound according to claim 1 wherein Z is an antigen and n is in the range of from 1 to 500.
Claim 4
Dependent Claim
18 words
Compound according to claim 1 wherein Z is an enzyme and n is of from 1 to 30.
Claim 5
Dependent Claim
57 words
Compound according to claim 1 and of the formula: (Ami--CH.sub.2 --R.sup.1 CO-).sbsb.n.sub.1 Z.sup.1 wherein: Ami is defined in claim 1; R 1 is alkylene of from 1 to 7 carbon atoms; n 1 is at least 1 and not greater than the molecular weight of Z 1 divided by 500; and Z 1 is a poly(amino acid).
Claim 6
Dependent Claim
22 words
Compound according to claim 4, wherein Z 1 is an enzyme and n 1 is in the range of 2 and 16.
Claim 7
Dependent Claim
10 words
Compound according to 5, wherein said enzyme is glucose-6-phosphate dehydrogenase.
Claim 8
Dependent Claim
10 words
Compound according to 5, wherein said enzyme is glucose-6-phosphate dehydrogenase.
Claim 9
Dependent Claim
23 words
Compound according to claim 4 wherein Z 1 is an antigen and n 1 is in the range of about 2 to 500.
Claim 10
Dependent Claim
11 words
Compound according to claim 7 wherein said antigen is a globulin.
Claim 11
Dependent Claim
10 words
Compound according to 7 wherein said antigen is an albumin.
Claim 12
Dependent Claim
10 words
Compound according to 7 wherein said antigen is an albumin.
Claim 13
Dependent Claim
9 words
The conjugate of 2- or 3-(γ-carboxypropyl)amitriptyline with glucose-6-phosphate dehydrogenase.
Claim 14
Dependent Claim
9 words
The conjugate of 2- or 3-(γ-carboxypropyl)amitriptyline with glucose-6-phosphate dehydrogenase.
Claim 15
Dependent Claim
11 words
The conjugate of 2- or 3-(γ-carboxypropyl)amitriptyline with a poly(amino acid) antigen.
Claim 16
Dependent Claim
11 words
The conjugate of 2- or 3-(γ-carboxypropyl)amitriptyline with a poly(amino acid) antigen.
Claim 17
Dependent Claim
11 words
Conjugate according to claim 11 wherein said antigen is a globulin.
Claim 18
Dependent Claim
11 words
Conjugate according to claim 11 wherein said antigen is an albumin.
Claim 19
Dependent Claim
20 words
Antibodies prepared in response to an antigen according to any of claims 4, 7 to 9 or 11 to 13.
References Cited (Backward Citations)
US3852157A
Cited
US4069105A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-09-16 | 1984-03-17 | 1984-09-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-09-16 | 1988-03-17 | 1988-09-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-09-16 | 1992-03-17 | 1992-09-16 | $7700.00 | Window Closed / Lapsed |