US4219645A
Expired / Lapsed
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Stabilized nicotinamide-adenine dinucleotides and process for their preparation
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1979-03-29
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Granted: 1980-08-26
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Expires: 1999-03-29
Abstract
Abstract Nicotinamide-adenine dinucleotide or the phosphate thereof, each in the reduced as well as the oxidized forms, are stabilized against activity losses in storage by the addition of hydrolytically decomposed, optionally cross-linked collagen.
Inventors
- 👤 Joseph Stark
- 👤 Reinhard Muller-Matthesius
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 09:50
CPC Classifications
C; C07; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/16; C07H19/20; C07H19/207; C12; C12Q; C12Q1/00
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
40 words
A stable mixture comprising (1) a member selected from the group consisting of oxidized nicotinamide-adenine dinucleotide, reduced nictonamide-adenine dinucleotide, oxidized nicotinamide-adenine dinucleotide phosphate, and reduced nicotinamide-adenine dinucleotide phosphate and (2) decomposed collagen having a molecular weight from 2000 to 20000.
Claim 2
Dependent Claim
40 words
A stable mixture comprising (1) a member selected from the group consisting of oxidized nicotinamide-adenine dinucleotide, reduced nictonamide-adenine dinucleotide, oxidized nicotinamide-adenine dinucleotide phosphate, and reduced nicotinamide-adenine dinucleotide phosphate and (2) decomposed collagen having a molecular weight from 2000 to 20000.
Claim 3
Dependent Claim
20 words
A stable mixture as in claim 1 wherein said decomposed collagen has been cross-linked by prior reaction with a diisocyanate.
Claim 4
Dependent Claim
47 words
A stable mixture as in claim 2 wherein said decomposed collagen has been cross-linked by prior reaction with an amount of diisocyanate which is from 20 to 80 percent of the theoretical amount required for reaction with amino groups and guanidino groups present in the decomposed collagen.
Claim 5
Dependent Claim
22 words
A stable mixture as in claim 1 wherein the ratio by weight of nucleotide to decomposed collagen is between 1:0.001 and 1:1200.
Claim 6
Dependent Claim
10 words
A stable mixture as in claim 1 which is lyophilized.
Claim 7
Independent Claim
50 words
A method for stabilizing a solution of a member selected from the group consisting of oxidized nicotinamide-adenine dinucleotide, reduced nicotinamide-adenine dinucleotide, oxidized nicotinamide-adenine dinucleotide phosphate, and reduced nicotinamide-adenine dinucleotide phosphate, which method comprises combining a solution of said member with decomposed collagen having a molecular weight from 2000 to 20,000.
Claim 8
Independent Claim
50 words
A method for stabilizing a solution of a member selected from the group consisting of oxidized nicotinamide-adenine dinucleotide, reduced nicotinamide-adenine dinucleotide, oxidized nicotinamide-adenine dinucleotide phosphate, and reduced nicotinamide-adenine dinucleotide phosphate, which method comprises combining a solution of said member with decomposed collagen having a molecular weight from 2000 to 20,000.
Claim 9
Dependent Claim
19 words
A method as in claim 6 wherein said decomposed collagen has been cross-linked by prior reaction with a diisocyanate.
Claim 10
Dependent Claim
13 words
A method as in claim 6 wherein the stabilized solution is then lyophilized.
Claim 11
Dependent Claim
13 words
A method as in claim 7 wherein the stabilized solution is then lyophilized.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-08-26 | 1984-02-25 | 1984-08-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-08-26 | 1988-02-25 | 1988-08-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-08-26 | 1992-02-25 | 1992-08-26 | $7700.00 | Window Closed / Lapsed |