Antibiotic production

Claim 2

A process for the production of S7481/F-1 of formula I, ##STR3## or S7481/F-2 of formula II, ##STR4## or S7481/F-1 and S7481/F-2, which comprises cultivating a S7481/F-1; S7481/F-2, or S7481/F-1 and S7481/F-2 producing strain of the fungus species, Cylindrocarpon lucidum Booth or Trichoderma polysporum (Link ex Pers.) Rifai (now referred to as Tolypocladium inflatum Gams) in contact with a nutrient medium and isolating S7481/F-1; S7481/F-2 or S7481/F-1 and S7481/F-2.

Claim 3

A process according to claim 1, wherein the strain is a Cylindrocarpon lucidum Booth strain.

Claim 4

A process according to claim 2, wherein the strain is NRRL 5760 an obvious taxonomical equivalent thereof, or a mutant or variant thereof.

Claim 5

A process according to claim 3, wherein the strain is NRRL 5760 or an obvious taxonomical equivalent thereof.

Claim 6

A process according to claim 1, wherein the strain is a Trichoderma polysporum (Link ex Pers.) Rifai strain.

Claim 7

A process according to claim 5, wherein the strain is NRRL 8044 or an obvious taxonomical equivalent thereof, or a mutant or variant thereof.

Claim 8

A process according to claim 6, wherein the strain is NRRL 8044 or an obvious taxonomical equivalent thereof.

Claim 9

A process according to claim 1 for the production of antibiotic S 7481/F-1 having the following characteristics when in amorphous form: colorless powder; M.P. 148°-151° C. (decomp.); [α] D 20 =-236° (CHCl 3 ; c=0.5); U.V. Spectrum [CH 3 OH; c=1.063 g/l; d=0.01 cm] as shown in FIG. 1 of the accompanying drawings; I.R. Spectrum [CH 2 Cl 2 ] as shown in FIG. 2 of the accompanying drawings; Proton N.M.R. Spectrum [90 MHz; CDCl 3 ] as shown in FIG. 3 of the accompanying drawings; Carbon-13 N.M.R. Spectrum [250 mg in 1.5 ml CDCl 3 ; sweep width 6000 MHz] as shown in accompanying Table; High resolution mass spectrum [ion acceleration 4 kv; temperature ion source 290° C.; pressure 5.10 -6 mm Hg] peak of highest mass=m/e 1183.831 [±0.004] corresponding to C 62 H 109 N 11 O 11 recognizable as product of elimination of water from C 62 H 111 N 11 O 12 ; Elementary analysis C 61.8, H 9.4, N 13.0, O 15.7% in agreement with empirical formula C 62 H 111 N 11 O 12 ; and release of amino acids alanine, α-aminobutyric acid, N-methylleucine, N-methylglycine and valine on reaction with 6 N aqueous hydrochloric acid at reflux, which comprises cultivating a S 7481/F-1; S7481/F-2, or S 7481/F-1 and S 7481/F-2 producing strain of the fungus species, Cylindrocarpon lucidum Booth or Trichoderma polysporum (Link ex Pers.) Rifai (now referred to as Tolypocladium inflatum Gams) in contact with a nutrient medium and separating S 7481/F-1 from S 7481/F-2.

Claim 10

A process according to claim 1 for the production of antibiotic S 7481/F-2 having the following characteristics when in amorphous form: Colorless powder; M.P. 127°-130° C. (decomp.); [α] C 20 =-243° (CHCl 3 , c=0.5); U.V. Spectrum [CH 3 OH; c=1.062 g/l; d=0.01 cm] as shown in FIG. 4 of the accompanying drawings; I.R. Spectrum [CH 2 Cl 2 ] as shown in FIG. 5 of the accompanying drawings; Proton N.M.R. Spectrum [90 MHz; CDCl 3 ] as shown in FIG. 6 of the accompanying drawings; High resolution mass Spectrum [ion acceleration 4 kv; ion source temperature 290° C.; pressure 5.10 -6 mm Hg] peak of highest mass m/e 1169.815 (±0.006) corresponding to C 61 H 107 N 11 O 11 , the product of water elimination from C 61 H 109 N 11 O 12 ; and Elementary analysis found C 61.7; H 9.1, N 13.1, O 16.5% in agreement with empirical formula C 61 H 109 N 11 O 12 , which comprises cultivating a S 7481/F-1; S 7481/F-2, or S 7481/F-1 and S 7481/F-2 producing strain of the fungus species, Cylindrocarpon lucidum Booth or Trichoderma polysporum (Link ex Pers.) Rifai (now referred to as Tolypocladium inflatum Gams) in contact with a nutrient medium and separating S 7481/F-2 from S 7481/F-1.