US4214049A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Assay of serine proteases using novel chromogenic enzyme substrates
Assignee: Phadia AB
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Filing: 1978-10-27
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Granted: 1980-07-22
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Expires: 1998-10-27
Abstract
Abstract Method for the quantitative determination of serine proteases which includes contacting the substance for which the determination is desired with a diagnostically active chromogenic substrate with good stability and high specificity to serine proteases having the formula: H-D-A1-A2-A3-NH-R or salts thereof, wherein A1 is Gly, Ala, Val, Leu, Ile, Pip, Pro, or Aze; A2 is Gly, Ala, Val, Leu, Ile, Pip, Pro, Aze, or Phe; A3 is Arg, Lys, or Orn, and R is a chromophoric group.
Inventors
- 👤 Bo T. Af Ekenstam
- 👤 Leif E. Aurell
- 👤 Karl G. Claeson
- 👤 Birgitta G. Karlsson
- 👤 Stig I. Gustavsson
- 👤 Gun A. Olausson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 02:57
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/56; C07; C07K; C07K5/00; C07K5/04; C07K5/08; C07K5/0802; C07K5/0804; C07K5/0808; C12Q1/34; C12Q1/37; C12Q2337/00; C12Q2337/10; C12Q2337/12
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
123 words
A method for the quantitative determination of serine proteases which comprises contacting the substrate for which said determination is desired with a diagnostically active chromogenic substrate with good solubility and a high specificity to serine proteases, having the following general formula: H--D--A.sub.1 --A.sub.2 --A.sub.3 --NH--R or salts thereof, wherein A 1 is an amino acid selected from the group consisting of Gly, Ala, Val, Leu, Ile, Pip, Pro, and Aze; A 2 is an amino acid selected from the group consisting of Gly, Ala, Val, Leu, Ile, Pip, Pro, Aze, and Phe; A 3 is an amino acid selected from the group consisting of Arg, Lys, and Orn; and R is a chromophoric group, and then spectrophotometrically measuring the degree of enzymatic hydrolysis.
Claim 2
Independent Claim
123 words
A method for the quantitative determination of serine proteases which comprises contacting the substrate for which said determination is desired with a diagnostically active chromogenic substrate with good solubility and a high specificity to serine proteases, having the following general formula: H--D--A.sub.1 --A.sub.2 --A.sub.3 --NH--R or salts thereof, wherein A 1 is an amino acid selected from the group consisting of Gly, Ala, Val, Leu, Ile, Pip, Pro, and Aze; A 2 is an amino acid selected from the group consisting of Gly, Ala, Val, Leu, Ile, Pip, Pro, Aze, and Phe; A 3 is an amino acid selected from the group consisting of Arg, Lys, and Orn; and R is a chromophoric group, and then spectrophotometrically measuring the degree of enzymatic hydrolysis.
Claim 3
Dependent Claim
15 words
The method of claim 1 wherein R is nitrophenyl, naphthyl, nitronaphthyl, methoxynaphthyl, quinolyl or nitroquinolyl.
Claim 4
Dependent Claim
9 words
The method of claim 1 wherein R is nitrophenyl.
Claim 5
Dependent Claim
15 words
The method of claim 3 wherein said spectrometrically measuring is carried out at 405 nm.
Claim 6
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Val-Pro-Arg-pNA, or salt thereof.
Claim 7
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Val-Pip-Arg-pNA, or salt thereof.
Claim 8
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Val-Leu-Arg-pNA, or salt thereof.
Claim 9
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Val-Leu-Lys-pNA, or salt thereof.
Claim 10
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Ile-Leu-Arg-pNA, or salt thereof.
Claim 11
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Ile-Leu-Lys-pNA, or salt thereof.
Claim 12
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Pro-Phe-Arg-pNA, or salt thereof.
Claim 13
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Val-Pip-Arg-pNA, or salt thereof.
Claim 14
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Pro-Phe-Lys-pNA, or salt thereof.
Claim 15
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Ala-Ala-Arg-pNA, or salt thereof.
Claim 16
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Leu-Gly-Arg-pNA, or salt thereof.
Claim 17
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Leu-Ile-Arg-pNA, or salt thereof.
Claim 18
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Leu-Val-Arg-pNA, or salt thereof.
Claim 19
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Leu-Val-Lys-pNA, or salt thereof.
Claim 20
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Pip-Phe-Arg-pNA, or salt thereof.
Claim 21
Dependent Claim
13 words
The method of claim 1 wherein said substrate is H-D-Leu-Leu-Arg-pNA, or salt thereof.
Claim 22
Dependent Claim
10 words
The method of claim 1 wherein A 1 is Val.
Claim 23
Dependent Claim
10 words
The method of claim 1 wherein A 1 is Ile.
Claim 24
Dependent Claim
10 words
The method of claim 1 wherein A 1 is Pro.
Claim 25
Dependent Claim
10 words
The method of claim 1 wherein A 2 is Pro.
Claim 26
Dependent Claim
10 words
The method of claim 1 wherein A 2 is Pip.
Claim 27
Dependent Claim
10 words
The method of claim 1 wherein A 2 is Leu.
Claim 28
Dependent Claim
10 words
The method of claim 1 wherein A 2 is Phe.
Claim 29
Dependent Claim
10 words
The method of claim 1 wherein A 3 is Arg.
Claim 30
Dependent Claim
10 words
The method of claim 1 wherein A 3 is Lys.
Claim 31
Dependent Claim
10 words
The method of claim 1 which is a hydrochloride salt.
Claim 32
Dependent Claim
15 words
The method of claim 1 wherein said spectrophotometrically measuring is carried out at 405 nm.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-07-22 | 1984-01-21 | 1984-07-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-07-22 | 1988-01-21 | 1988-07-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-07-22 | 1992-01-21 | 1992-07-22 | $7700.00 | Window Closed / Lapsed |