US4212946A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for recovering protease
Assignee: Sagami Chemical Research Institute
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Filing: 1978-05-18
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Granted: 1980-07-15
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Expires: 1998-05-18
Abstract
Abstract Protease is recovered by reacting a first amino acid whose amino group is protected with a protective group with a second amino acid whose carboxyl group is protected with a protective group, in an aqueous medium in the presence of a protease to result a peptide synthesis to deposit the addition compound of a dipeptide and said second C-terminal protected amino acid; dissolving said addition compound by adding an organic solvent to a precipitate separated from the reaction mixture and isolating the protease from an organic solvent suspension or dissolving said addition compound by adding a water immiscible organic solvent and separating the organic solvent phase by a liquid-liquid separation. The addition compound can be dissolved in an organic solvent after separating the precipitate from the reaction mixture. The addition compound can be also dissolved by adding a water-immiscible organic solvent to the reaction mixture and the organic solvent phase can be separated from the aqueous phase.
Inventors
- 👤 Yuji Nonaka
- 👤 Kiyotaka Oyama
- 👤 Heijiro Satoh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 20:08
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12P; C12P21/00; C12P21/02; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
95 words
A process for recovering protease which comprises reacting a first amino acid whose amino group is protected with a protective group with a second amino acid whose carboxyl group is protected with a protective group in an aqueous medium in the presence of the protease to result in a peptide synthesis and to deposit as a precipitate the addition compound of a dipeptide and said second C-terminal protected amino acid; dissolving said addition compound by adding an organic solvent to a precipitate separated from the reaction mixture and isolating the protease from said organic solvent.
Claim 2
Dependent Claim
95 words
A process for recovering protease which comprises reacting a first amino acid whose amino group is protected with a protective group with a second amino acid whose carboxyl group is protected with a protective group in an aqueous medium in the presence of the protease to result in a peptide synthesis and to deposit as a precipitate the addition compound of a dipeptide and said second C-terminal protected amino acid; dissolving said addition compound by adding an organic solvent to a precipitate separated from the reaction mixture and isolating the protease from said organic solvent.
Claim 3
Dependent Claim
70 words
A process according to claim 1 wherein the protective group for the amino group of the first amino acid or peptide is an aliphatic oxycarbonyl group, a benzloxycarbonyl group which can have ring substituents, a benzoyl group, an aromatic sulfonyl group or an aromatic sulfuryl group; and the protective group for the carboxyl group of the second amino acid or peptide is a lower alkoxy group or a benzhydryloxy group.
Claim 4
Dependent Claim
13 words
A process according to claim 1 wherein the protease is a metallo protease.
Claim 5
Dependent Claim
60 words
A process according to claim 1 wherein the precipitate is the addition compound having the formula ##STR2## wherein A represents a residue of asparatic acid or glutamic acid exluding C-terminal and N-terminal and B represents a residue of the second amino acid excluding C-terminal and N-terminal and X represents an N-terminal protective group and Y represents a C-terminal protective group.
Claim 6
Dependent Claim
33 words
A process according to claim 1 wherein the first amino acid is asparatic acid or glutamic acid and the second amino acid or peptide is a monoaminomonocarboxylic acid having no other functional group.
Claim 7
Dependent Claim
17 words
A process according to claim 1 wherein the monoaminomonocarboxylic acid having no other functional group is phenylalanine.
Claim 8
Dependent Claim
12 words
A process according to claim 1 wherein the aqueous medium is water.
Claim 9
Dependent Claim
101 words
A process for recovering protease which comprises reacting a first amino acid whose amino group is protected with a protective group with a second amino acid whose carboxyl group is protected with a protective group in an aqueous medium in the presence of the protease to result in a peptide synthesis and to deposit as a precipitate the addition compound of a dipeptide and said second C-terminal protected amino acid; dissolving said precipitate by adding a water immiscible organic solvent to said aqueous medium; separating the resulting organic phase from the aqueous phase; and separating said protease from said aqueous phase.
Claim 10
Dependent Claim
101 words
A process for recovering protease which comprises reacting a first amino acid whose amino group is protected with a protective group with a second amino acid whose carboxyl group is protected with a protective group in an aqueous medium in the presence of the protease to result in a peptide synthesis and to deposit as a precipitate the addition compound of a dipeptide and said second C-terminal protected amino acid; dissolving said precipitate by adding a water immiscible organic solvent to said aqueous medium; separating the resulting organic phase from the aqueous phase; and separating said protease from said aqueous phase.
Claim 11
Dependent Claim
68 words
A process according to claim 8, wherein the protective group for the amino group of the first amino acid or peptide is an aliphatic oxycarbonyl group, a benzyloxycarbonyl group which can have ring substituents, a benzoyl group, an aromatic sulfonyl group or aromatic sulfuryl group; and the protective group for the carboxyl group of the second amino acid or peptide is lower alkoxy group or a benzhydryloxy group.
References Cited (Backward Citations)
US4086136A
Cited
US4119493A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-07-15 | 1984-01-14 | 1984-07-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-07-15 | 1988-01-14 | 1988-07-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-07-15 | 1992-01-14 | 1992-07-15 | $7700.00 | Window Closed / Lapsed |