US4209373A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Acidic agar gel electrochromatography of hemoglobins
Assignee: Bayer Corp
•
Filing: 1979-06-01
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Granted: 1980-06-24
•
Expires: 1999-06-01
Abstract
Abstract The common hemoglobin variants A, A 2 , F, S, and C are discretely separated by electrochromatography under acidic conditions on an agar gel which utilizes an agar gel having a wet thickness of from about 0.1 to about 0.5 mm. and a potential of from about 60 to about 110 volts. The gel buffer is a citrate buffer having a citrate concentration of from about 0.04 to about 0.065 M and a pH of from about 6.0 to about 6.5. The well buffer is a citrate buffer having a citrate concentration of from about 0.055 to about 0.065 M and a pH of from about 6.0 to about 6.5.
Inventors
- 👤 Barry I. Bluestein
- 👤 Cyrus A. Lepp
- 👤 Robert D. Mason
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 20:51
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
89 words
In a method for discretely separating the common hemoglobin variants A, A 2 , F, S, and C by electrochromatography on an agar gel, under acidic conditions utilizing citrate gel and well buffers, each of which has a citrate concentration of approximately 0.05 M and a pH of approximately 6.0, and a potential of 50-90 volts, the improvement which comprises employing: A. an agar gel having a wet thickness of from about 0.1 to about 0.5 mm. and B. a potential of from about 60 to about 110 volts.
Claim 2
Dependent Claim
89 words
In a method for discretely separating the common hemoglobin variants A, A 2 , F, S, and C by electrochromatography on an agar gel, under acidic conditions utilizing citrate gel and well buffers, each of which has a citrate concentration of approximately 0.05 M and a pH of approximately 6.0, and a potential of 50-90 volts, the improvement which comprises employing: A. an agar gel having a wet thickness of from about 0.1 to about 0.5 mm. and B. a potential of from about 60 to about 110 volts.
Claim 3
Dependent Claim
19 words
The method of claim 1 in which the gel wet thickness is from about 0.3 to about 0.4 mm.
Claim 4
Dependent Claim
13 words
The method of claim 1 in which the potential is about 90 volts.
Claim 5
Dependent Claim
25 words
The method of claim 1 in which the gel contains a humectant at a level of from about 0 to about 10% weight per volume.
Claim 6
Dependent Claim
11 words
The method of claim 4 in which the humectant is sucrose.
Claim 7
Dependent Claim
20 words
The method of claim 5 in which the sucrose is present at a level of about 5%, weight per volume.
Claim 8
Dependent Claim
26 words
The method of claim 1 in which the gel contains a wetting agent at a level of from about 0 to about 0.1%, weight per volume.
Claim 9
Dependent Claim
13 words
The method of claim 7 in which the wetting agent is polyvinyl alcohol.
Claim 10
Dependent Claim
21 words
The method of claim 8 in which the polyvinyl alcohol is present at a level of about 0.02%, weight per volume.
Claim 11
Dependent Claim
23 words
The method of claim 1 in which the gel contains agarose at a level of from 0 to about 10%, weight per volume.
Claim 12
Dependent Claim
20 words
The method of claim 10 in which the agarose is present at a level of about 0.5%, weight per volume.
Claim 13
Dependent Claim
108 words
In a method for discretely separating the common hemoglobin variants A, A 2 , F, S, and C by electrochromatography on an agar gel, under acidic conditions utilizing citrate gel and well buffers, each of which has a citrate concentration of approximately 0.05 M and a pH of approximately 6.0, a potential of 50-90 volts, and a hemoglobin-specific stain to visualize the separated hemoglobin variants, the improvement which comprises employing: A. an agar gel having a wet thickness of from about 0.1 to about 0.5 mm., B. a potential of from about 60 to about 110 volts, and C. a nonspecific protein stain to visualize the separated hemoglobins.
Claim 14
Dependent Claim
108 words
In a method for discretely separating the common hemoglobin variants A, A 2 , F, S, and C by electrochromatography on an agar gel, under acidic conditions utilizing citrate gel and well buffers, each of which has a citrate concentration of approximately 0.05 M and a pH of approximately 6.0, a potential of 50-90 volts, and a hemoglobin-specific stain to visualize the separated hemoglobin variants, the improvement which comprises employing: A. an agar gel having a wet thickness of from about 0.1 to about 0.5 mm., B. a potential of from about 60 to about 110 volts, and C. a nonspecific protein stain to visualize the separated hemoglobins.
Claim 15
Dependent Claim
19 words
The method of claim 12 in which the gel wet thickness is from about 0.3 to about 0.4 mm.
Claim 16
Dependent Claim
13 words
The method of claim 12 in which the potential is about 90 volts.
Claim 17
Dependent Claim
14 words
The method of claim 12 in which the nonspecific protein stain is amido black.
Claim 18
Dependent Claim
25 words
The method of claim 12 in which the gel contains a humectant at a level of from about 0 to about 10%, weight per volume.
Claim 19
Dependent Claim
11 words
The method of claim 16 in which the humectant is sucrose.
Claim 20
Dependent Claim
20 words
The method of claim 17 in which the sucrose is present at a level of about 5%, weight per volume.
Claim 21
Dependent Claim
26 words
The method of claim 12 in which the gel contains a wetting agent at a level of from about 0 to about 0.1%, weight per volume.
Claim 22
Dependent Claim
13 words
The method of claim 19 in which the wetting agent is polyvinyl alcohol.
Claim 23
Dependent Claim
21 words
The method of claim 20 in which the polyvinyl alcohol is present at a level of about 0.02%, weight per volume.
Claim 24
Dependent Claim
23 words
The method of claim 12 in which the gel contains agarose at a level of from 0 to about 1%, weight per volume.
Claim 25
Dependent Claim
20 words
The method of claim 22 in which the agarose is present at a level of about 0.5%, weight per volume.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-06-24 | 1983-12-24 | 1984-06-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-06-24 | 1987-12-24 | 1988-06-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-06-24 | 1991-12-24 | 1992-06-24 | $7700.00 | Window Closed / Lapsed |