Patent US2290407A successfully refreshed from Google Patents.
US4207166A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for electrophoresis
Assignee: Individual
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Filing: 1977-06-09
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Granted: 1980-06-10
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Expires: 1997-06-09
Abstract
Abstract A plate for use in electrophoresis, comprising a membrane coated with a layer of an electrophoretic medium such as agarose gel. The membrane is bonded to a supporting sheet having cutouts in which the electrophoresis is performed. A transport apparatus moves the plates through an electrophoresis bath into an optical scanning densitometer.
Inventors
- 👤 Harald Dahms
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 21:38
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44704
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
147 words
An apparatus for electrophoretic analysis of biological samples comprising: input means for receiving and holding a plate to be used in said analysis, said plate having an electrophoretic medium and said biological sample thereon, support means for supporting an electrophoretic bath which said plate contacts during said analysis, electric means for applying an electric field across said plate while said plate is in contact with said bath, said electric means including means for electrically contacting said bath, and mechanical means connected to said input means for bringing said plate and said bath into electrolytic contact with one another for a time sufficient to allow electrophoresis to occur and for providing relative motion between said plate and said bath, said electric field being applied in a direction which is transverse to said relative motion to produce electrophoretic separations which extend in a direction transverse to said relative motion.
Claim 2
Independent Claim
147 words
An apparatus for electrophoretic analysis of biological samples comprising: input means for receiving and holding a plate to be used in said analysis, said plate having an electrophoretic medium and said biological sample thereon, support means for supporting an electrophoretic bath which said plate contacts during said analysis, electric means for applying an electric field across said plate while said plate is in contact with said bath, said electric means including means for electrically contacting said bath, and mechanical means connected to said input means for bringing said plate and said bath into electrolytic contact with one another for a time sufficient to allow electrophoresis to occur and for providing relative motion between said plate and said bath, said electric field being applied in a direction which is transverse to said relative motion to produce electrophoretic separations which extend in a direction transverse to said relative motion.
Claim 3
Dependent Claim
22 words
The apparatus of claim 1, where said mechanical means for providing relative motion transports said plate into electrolytic contact with said bath.
Claim 4
Dependent Claim
24 words
The apparatus of claim 2, where said direction of transport of said plate is substantially perpendicular to the direction of said applied electric field.
Claim 5
Dependent Claim
39 words
The apparatus of claim 1, further including optical scanning means to which said plate is brought by said mechanical means after said electric field is applied thereacross, and for scanning said separations along the direction in which they extend.
Claim 6
Dependent Claim
15 words
The apparatus of claim 4, wherein said scanning means includes a source of ultraviolet light.
Claim 7
Dependent Claim
20 words
The apparatus of claim 4, where said plate has at least one sample well therein for containing said biological sample.
Claim 8
Dependent Claim
25 words
The apparatus of claim 1, including additional input means connected to said mechanical means for receiving additional plates to be used in said electrophoretic analysis.
Claim 9
Dependent Claim
28 words
The apparatus of claim 1, further including means for controlling the amount of time during which said plate and said bath are in electrolytic contact with one another.
Claim 10
Independent Claim
136 words
An apparatus for performing electrophoretic analysis of biological samples, comprising: input means for receiving and holding a plate having an electrophoretic medium and said biological sample thereon, transport means connected to said input means for moving said plate in a first direction, electric means for applying an electric field across said plate to produce electrophoretic separations of the components of said sample, said electric means being comprised of electrophoretic baths for applying said electric field across said plate in a direction transverse to the direction of movement of said plate when said plate is brought into electrolytic contact with said baths by said transport means, optical scanning means for scanning said separations for analysis of said components electrophoretically separated by said electric field when said plate is transported to said scanning means by said transport means.
Claim 11
Independent Claim
136 words
An apparatus for performing electrophoretic analysis of biological samples, comprising: input means for receiving and holding a plate having an electrophoretic medium and said biological sample thereon, transport means connected to said input means for moving said plate in a first direction, electric means for applying an electric field across said plate to produce electrophoretic separations of the components of said sample, said electric means being comprised of electrophoretic baths for applying said electric field across said plate in a direction transverse to the direction of movement of said plate when said plate is brought into electrolytic contact with said baths by said transport means, optical scanning means for scanning said separations for analysis of said components electrophoretically separated by said electric field when said plate is transported to said scanning means by said transport means.
Claim 12
Dependent Claim
24 words
The apparatus of claim 9, where said electric field is applied in a direction substantially perpendicular to the direction of movement of said plate.
Claim 13
Dependent Claim
29 words
The apparatus of claim 9, where said means for holding includes means for holding said plate in a bent position, opposing edges of said plate contacting separate electrophoretic baths.
Claim 14
Dependent Claim
33 words
The apparatus of claim 11, where said plate has at least one sample well therein for said sample, and said optical scanning means includes a source of ultraviolet radiation which scans said separations.
Claim 15
Independent Claim
111 words
An apparatus for performing electrophoretic analysis of biological samples, comprising: input means for receiving and holding a plate having an electrophoretic medium and said biological sample thereon, transport means connected to said input means for moving said plate in a first direction to an electric means and then to an optical scanning means, electric means for applying an electric field across said plate to produce electrophoretic separations of the components of said sample, said electric field being applied across said plate in a direction transverse to the direction of movement of said plate, optical scanning means for scanning said separations for analysis of said components electrophoretically separated by said electric field.
Claim 16
Independent Claim
111 words
An apparatus for performing electrophoretic analysis of biological samples, comprising: input means for receiving and holding a plate having an electrophoretic medium and said biological sample thereon, transport means connected to said input means for moving said plate in a first direction to an electric means and then to an optical scanning means, electric means for applying an electric field across said plate to produce electrophoretic separations of the components of said sample, said electric field being applied across said plate in a direction transverse to the direction of movement of said plate, optical scanning means for scanning said separations for analysis of said components electrophoretically separated by said electric field.
Claim 17
Dependent Claim
20 words
The apparatus of claim 13, where said optical scanning means includes means for providing ultraviolet radiation for scanning said separations.
Claim 18
Independent Claim
144 words
An apparatus for performing electrophoretic analysis of biological samples, comprising: input means for receiving and holding a support member containing an electrophoretic medium and said biological sample, said support member being substantially transparent to ultraviolet radiation, transport means connected to said input means for moving said support member in a first direction to an electric means and then to an optical scanning means, electric means for applying an electric field across said support member to produce electrophoretic separations of the components of said sample, said electric means being comprised of electrophoretic baths for applying said electric field across said support member in a direction transverse to the direction of movement of said support member when said baths are contacted by said support member, optical scanning means for scanning said separations with ultraviolet radiation for analysis of said components electrophoretically separated by said electric field.
Claim 19
Independent Claim
144 words
An apparatus for performing electrophoretic analysis of biological samples, comprising: input means for receiving and holding a support member containing an electrophoretic medium and said biological sample, said support member being substantially transparent to ultraviolet radiation, transport means connected to said input means for moving said support member in a first direction to an electric means and then to an optical scanning means, electric means for applying an electric field across said support member to produce electrophoretic separations of the components of said sample, said electric means being comprised of electrophoretic baths for applying said electric field across said support member in a direction transverse to the direction of movement of said support member when said baths are contacted by said support member, optical scanning means for scanning said separations with ultraviolet radiation for analysis of said components electrophoretically separated by said electric field.
Claim 20
Independent Claim
153 words
An apparatus for electrophoretic analysis of a biological sample located on an electrophoresis cell having an electrophoretic medium thereon, comprising: transport means for holding said cell and for moving said cell in response to a control signal, said transport means moving said cell into electrolytic contact with electrophoretic baths and then to an optical scanner, electric means connected to said baths for providing electrolytic contact thereto when said cell is in electrolytic contact with said baths, said electrolytic contact producing an electric field across said cell in a direction transverse to the direction of movement of said cell to produce electrophoretic separations of the constituents in said biological sample, said separations extending in a direction along said electric field and transverse to said direction of motion of said cell, and optical scanning means for optically scanning said electrophoretic separations in response to a control signal to determine the constituents in said biological sample.
Claim 21
Independent Claim
153 words
An apparatus for electrophoretic analysis of a biological sample located on an electrophoresis cell having an electrophoretic medium thereon, comprising: transport means for holding said cell and for moving said cell in response to a control signal, said transport means moving said cell into electrolytic contact with electrophoretic baths and then to an optical scanner, electric means connected to said baths for providing electrolytic contact thereto when said cell is in electrolytic contact with said baths, said electrolytic contact producing an electric field across said cell in a direction transverse to the direction of movement of said cell to produce electrophoretic separations of the constituents in said biological sample, said separations extending in a direction along said electric field and transverse to said direction of motion of said cell, and optical scanning means for optically scanning said electrophoretic separations in response to a control signal to determine the constituents in said biological sample.
Claim 22
Independent Claim
146 words
An apparatus for electrophoretic analysis of a sample of body fluid, comprising: an electrophoresis cell having an electrophoretic medium and said body fluid sample thereon, transport means for holding said cell and for moving said cell into electrophoretic contact with electrophoretic baths for establishing an electric field across said sample and said electrophoretic medium, said cell being comprised of a plate having portions thereof which are transparent to electromagnetic radiation, where said transport means brings the edges of said plate into electrolytic contact with said baths, electric means including said baths for producing an electric field across said electrophoretic medium and said sample in a direction transverse to the direction of movement of said cell to produce electrophoretic separations of the constituents in said sample, optical scanning means for scanning said separations through said transparent portions of said plate to determine the constituents in said sample.
Claim 23
Independent Claim
146 words
An apparatus for electrophoretic analysis of a sample of body fluid, comprising: an electrophoresis cell having an electrophoretic medium and said body fluid sample thereon, transport means for holding said cell and for moving said cell into electrophoretic contact with electrophoretic baths for establishing an electric field across said sample and said electrophoretic medium, said cell being comprised of a plate having portions thereof which are transparent to electromagnetic radiation, where said transport means brings the edges of said plate into electrolytic contact with said baths, electric means including said baths for producing an electric field across said electrophoretic medium and said sample in a direction transverse to the direction of movement of said cell to produce electrophoretic separations of the constituents in said sample, optical scanning means for scanning said separations through said transparent portions of said plate to determine the constituents in said sample.
Claim 24
Dependent Claim
12 words
The apparatus of claim 17, where said electromagnetic radiation is ultraviolet light.
Claim 25
Dependent Claim
24 words
The apparatus of claim 18, where said plate is sufficiently flexible to be bent for electrolytic contact with said baths at opposing edges thereof.
Claim 26
Independent Claim
111 words
An apparatus for performing electrophoretic analysis of biological samples, comprising: input means for receiving and holding a cell having an electrophoretic medium and said biological sample thereon, transport means connected to said input means for moving said cell in a first direction to an electric means and then to an optical scanning means, electric means for applying an electric field across said cell to produce electrophoretic separations of the components of said sample, said electric field being applied across said cell in a direction transverse to the direction of movement of said cell, optical scanning means for scanning said separations for analysis of said components electrophoretically separated by said electric field.
Claim 27
Independent Claim
111 words
An apparatus for performing electrophoretic analysis of biological samples, comprising: input means for receiving and holding a cell having an electrophoretic medium and said biological sample thereon, transport means connected to said input means for moving said cell in a first direction to an electric means and then to an optical scanning means, electric means for applying an electric field across said cell to produce electrophoretic separations of the components of said sample, said electric field being applied across said cell in a direction transverse to the direction of movement of said cell, optical scanning means for scanning said separations for analysis of said components electrophoretically separated by said electric field.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-06-10 | 1983-12-10 | 1984-06-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-06-10 | 1987-12-10 | 1988-06-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-06-10 | 1991-12-10 | 1992-06-10 | $7700.00 | Window Closed / Lapsed |