US4204989A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Isolation of hepatitis B e antigen
Assignee: Merck and Co Inc
•
Filing: 1978-12-13
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Granted: 1980-05-27
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Expires: 1998-12-13
Abstract
Abstract Hepatitis B e antigen or hepatitis B e antibody are separated from human biological fluid by centrifugation in a density gradient and isolated in the 1.04-1.15 g/cc density region.
Inventors
- 👤 William J. McAleer
- 👤 Edward H. Wasmuth
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 17:16
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/576; G01N33/5761; C12; C12N; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
88 words
A method for isolating hepatitis B e antigen or hepatitis B e antibody which comprises isopycnically banding human biological fluid which contains hepatitis B surface antigen, recovering a fraction having a density range of from about 1.04 to about 1.15 g/cc, and and isolating HB e Ab if the plasma has an HB s Ag titer measured by complement fixation of at least about 256, or isolating HB e Ag if the plasma has an HB s Ag titer measured by complement fixation of less than about 128.
Claim 2
Independent Claim
88 words
A method for isolating hepatitis B e antigen or hepatitis B e antibody which comprises isopycnically banding human biological fluid which contains hepatitis B surface antigen, recovering a fraction having a density range of from about 1.04 to about 1.15 g/cc, and and isolating HB e Ab if the plasma has an HB s Ag titer measured by complement fixation of at least about 256, or isolating HB e Ag if the plasma has an HB s Ag titer measured by complement fixation of less than about 128.
Claim 3
Dependent Claim
14 words
A method according to claim 1 wherein the human biological fluid is clarified plasma.
Claim 4
Dependent Claim
18 words
A method according to claim 1 wherein the isopycnic banding is effected in an aqueous solution of NaBr.
Claim 5
Independent Claim
105 words
A method for isolating hepatitis B e antigen which comprises isopycnically banding human biological fluid which has a hepatitis B surface antigen titer measured by complement fixation of at least about 256, recovering a first fraction having a density range of from about 1.04 to about 1.15 g/cc, recovering a second fraction in the density range of from about 1.17 to about 1.22 g/cc, dialyzing and concentrating the second fraction, subjecting the concentrated second fraction to rate zonal banding and recovering a third fraction having a density range of from about 1.04 to about 1.15 g/cc, and dialyzing and concentrating the first and third fractions.
Claim 6
Independent Claim
105 words
A method for isolating hepatitis B e antigen which comprises isopycnically banding human biological fluid which has a hepatitis B surface antigen titer measured by complement fixation of at least about 256, recovering a first fraction having a density range of from about 1.04 to about 1.15 g/cc, recovering a second fraction in the density range of from about 1.17 to about 1.22 g/cc, dialyzing and concentrating the second fraction, subjecting the concentrated second fraction to rate zonal banding and recovering a third fraction having a density range of from about 1.04 to about 1.15 g/cc, and dialyzing and concentrating the first and third fractions.
Claim 7
Dependent Claim
14 words
A method according to claim 4 wherein the human biological fluid is clarified plasma.
Claim 8
Independent Claim
109 words
A method for isolating hepatitis B e antibody which comprises isopycnically banding human biological fluid which has a hepatitis B surface antigen titer as measured by complement fixation of less than about 128, recovering a first fraction having a density range of from about 1.04 to about 1.15 g/cc, recovering a second fraction in the density range of from about 1.17 to about 1.22 g/cc, dialyzing and concentrating the second fraction, subjecting the concentrated second fraction to rate zonal banding and recovering a third fraction having a density range of from about 1.04 to about 1.04 to about 1.15 g/cc, and dialyzing and concentrating the first and third fractions.
Claim 9
Independent Claim
109 words
A method for isolating hepatitis B e antibody which comprises isopycnically banding human biological fluid which has a hepatitis B surface antigen titer as measured by complement fixation of less than about 128, recovering a first fraction having a density range of from about 1.04 to about 1.15 g/cc, recovering a second fraction in the density range of from about 1.17 to about 1.22 g/cc, dialyzing and concentrating the second fraction, subjecting the concentrated second fraction to rate zonal banding and recovering a third fraction having a density range of from about 1.04 to about 1.04 to about 1.15 g/cc, and dialyzing and concentrating the first and third fractions.
Claim 10
Dependent Claim
14 words
A method according to claim 6 wherein the human biological fluid is clarified plasma.
Claim 11
Dependent Claim
14 words
A method according to claim 3 wherein the aqueous solution is a density gradient.
Claim 12
Dependent Claim
18 words
A method according to claim 4 wherein the isopycnic banding is effected in an aqueous solution of NaBr.
Claim 13
Dependent Claim
18 words
A method according to claim 6 wherein the isopycnic banding is effected in an aqueous solution of NaBr.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-05-27 | 1983-11-26 | 1984-05-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-05-27 | 1987-11-26 | 1988-05-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-05-27 | 1991-11-26 | 1992-05-27 | $7700.00 | Window Closed / Lapsed |