US4203801A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell and virus culture systems
Assignee: Mallinckrodt Veterinary Inc
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Filing: 1977-04-01
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Granted: 1980-05-20
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Expires: 1997-04-01
Abstract
Abstract A cell culture system which comprises living eucaryotic cells, such as those of human or animal origin, or mycophyta dispersed within a solid carrier body or bed, which carrier consists of porous or particulate material capable of retaining the cells while allowing liquid media to pass through or to have contact with the said cells, and processes for preparing and maintaining such culture systems, particularly for the purpose of virus propagation.
Inventors
- 👤 Ronald C. Telling
- 👤 Roy J. Passingham
- 👤 Brian L. Kitchener
- 👤 David G. Hopkinson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 22:59
CPC Classifications
C; C12; C12M; C12M25/00; C12M25/16; C12M23/00; C12M23/20; C12N; C12N5/00; C12N5/0068; C12N5/0075; C12N7/00; C12N2531/00; C12N2533/00; C12N2533/10; C12N2533/50; C12N2770/00; C12N2770/00011; C12N2770/32011; C12N2770/32051
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
82 words
A cell culture system which comprises living eucaryotic cells of human or animal origin, or mycophyta, dispersed within a porous carrier bed, said carrier bed comprising particulate material, and a support means for said carrier bed, said particulate material providing sufficient internal cavities or spaces for retaining and permitting said cells to grow and multiply within said carrier bed, and said support means allowing liquid media to pass through and out of said carrier bed and to have contact with said cells.
Claim 2
Dependent Claim
82 words
A cell culture system which comprises living eucaryotic cells of human or animal origin, or mycophyta, dispersed within a porous carrier bed, said carrier bed comprising particulate material, and a support means for said carrier bed, said particulate material providing sufficient internal cavities or spaces for retaining and permitting said cells to grow and multiply within said carrier bed, and said support means allowing liquid media to pass through and out of said carrier bed and to have contact with said cells.
Claim 3
Dependent Claim
23 words
A cell culture system according to claim 1 wherein the particle retention size of said carrier bed is from 0.1 to 2.00 μm.
Claim 4
Dependent Claim
16 words
A cell culture system according to claim 1 wherein said particulate material is a diatomaceous earth.
Claim 5
Dependent Claim
22 words
A cell culture system according to claim 1 wherein said diatomaceous earth is selected from the class consisting of diatomite and kieselguhr.
Claim 6
Dependent Claim
21 words
A cell culture system according to claim 1 wherein said carrier bed comprises more than one horizontal layer of particulate material.
Claim 7
Dependent Claim
34 words
A cell culture system according to claim 5 wherein the particle retention size of said layers of particulate material generally decreases from the top surface layer to the layer adjacent the bottom surface layer.
Claim 8
Dependent Claim
22 words
A cell culture system according to claim 6 wherein the particle retention size of said layers is from 0.1 to 0.75 μm.
Claim 9
Dependent Claim
22 words
A cell culture system according to claim 6 wherein the particle retention size of said layers is from 0.2 to 0.5 μm.
Claim 10
Dependent Claim
24 words
A cell culture system according to claim 6 wherein said bottom surface layer is of greater particle retention size than the layer adjacent thereto.
Claim 11
Dependent Claim
22 words
A cell culture system according to claim 9 wherein the particle retention size of said bottom surface layer is about 0.5 μm.
Claim 12
Dependent Claim
25 words
A cell culture system according to claim 1 wherein said carrier bed comprises a single layer of particle retention size 0.75 μm to 2.0 μm.
Claim 13
Dependent Claim
16 words
A cell culture system according to claim 11 wherein the particle retention size is 1.2 μm.
Claim 14
Dependent Claim
25 words
A cell culture system according to claim 1 wherein the thickness of a layer of said carrier bed is from 8 mm to 20 mm.
Claim 15
Dependent Claim
33 words
A cell culture system according to claim 1 wherein said eucaryotic cells are selected from the class consisting of baby hamster clone 21 cell line and IB2 Renal Swine pig kidney cell line.
Claim 16
Dependent Claim
16 words
A cell culture system according to claim 1 wherein said cells are infected with a microorganism.
Claim 17
Dependent Claim
14 words
A cell culture system according to claim 15 wherein said microorganism is a virus.
Claim 18
Dependent Claim
18 words
A cell culture system according to claim 16 wherein said virus is a strain of foot-&-mouth disease virus.
Claim 19
Dependent Claim
20 words
A cell culture system according to claim 1 wherein said carrier bed is supported on a horizontal plate pressure filter.
Claim 20
Dependent Claim
19 words
A cell culture system according to claim 1 wherein said carrier bed is supported on a porous support means.
Claim 21
Dependent Claim
34 words
A cell culture system according to claim 1 wherein said carrier bed is supported on a support means having means for allowing said liquid media to pass through and out of said carrier bed.
Claim 22
Dependent Claim
92 words
A recirculating cell culture system which comprises living eucaryotic cells of human or animal origin, or mycophyta, dispersed within a porous carrier bed, said carrier bed comprising particulate material, said particulate material providing sufficient internal cavities or spaces for retaining and permitting said cells to grow and multiply within said carrier bed, a support means for said carrier bed, said support means allowing liquid media to pass through and out of said carrier bed and to have contact with said cells, and means for recirculating said liquid media through said carrier bed.
Claim 23
Dependent Claim
92 words
A recirculating cell culture system which comprises living eucaryotic cells of human or animal origin, or mycophyta, dispersed within a porous carrier bed, said carrier bed comprising particulate material, said particulate material providing sufficient internal cavities or spaces for retaining and permitting said cells to grow and multiply within said carrier bed, a support means for said carrier bed, said support means allowing liquid media to pass through and out of said carrier bed and to have contact with said cells, and means for recirculating said liquid media through said carrier bed.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-05-20 | 1983-11-19 | 1984-05-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-05-20 | 1987-11-19 | 1988-05-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-05-20 | 1991-11-19 | 1992-05-20 | $7700.00 | Window Closed / Lapsed |