US4201694A
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US
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Method for increasing shelf-life of a serum bilirubin reference composition and composition produced thereby
Assignee: Beckman Coulter Inc
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Filing: 1978-07-17
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Granted: 1980-05-06
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Expires: 1998-07-17
Abstract
Abstract A stable blood serum bilirubin reference composition characterized in that said composition either (1) possesses a pH of from about 8.3 to about 9.5 or (2) further comprises a sulfhydryl compound in an amount sufficient to enhance the stability of the bilirubin or (3) both possesses a pH of from about 8.3 to about 9.5 and further comprises a sulfhydryl compound in an amount to further enhance the stability of the bilirubin.
Inventors
- 👤 Allan L. Louderback
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 19:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; G01N33/72; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/103332; Y10T436/108331
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
51 words
An improved blood serum reference composition of the type comprising blood serum having a bilirubin constituent of known value, characterized in that said composition possesses a pH of from about 8.3 to about 9.5 and further comprising a sulfhydryl compound in an amount sufficient to further enhance the stability of bilirubin.
Claim 2
Dependent Claim
51 words
An improved blood serum reference composition of the type comprising blood serum having a bilirubin constituent of known value, characterized in that said composition possesses a pH of from about 8.3 to about 9.5 and further comprising a sulfhydryl compound in an amount sufficient to further enhance the stability of bilirubin.
Claim 3
Dependent Claim
30 words
The composition of claim 1 wherein said sulfhydryl compound is present in an amount such that the oxidation-reduction potential of said composition is from about -30 to about -300 millivolts.
Claim 4
Dependent Claim
44 words
The composition of claim 2 wherein said sulfhydryl compound is present in an amount such that the oxidation-reduction potential of said standard is from about -100 to about -200 millivolts and wherein said composition possesses a pH of from about 8.3 to about 8.7.
Claim 5
Dependent Claim
36 words
The composition of claim 3 wherein said sulfhydryl compound is present in an amount such that the oxidation-reduction potential of said standard is about -160 millivolts and wherein said composition possesses a pH of about 8.5.
Claim 6
Dependent Claim
28 words
The composition of claim 4 wherein said sulfhydryl compound is selected from a group consisting of dithioerythreitol, dithiothreitol, mercaptoethanol, cysteine, reduced gluthionine, N-acetyl cysteine, mercaptoacetate, and mixtures thereof.
Claim 7
Dependent Claim
11 words
The composition of claim 5 wherein said sulfhydryl compound is dithiothreitol.
Claim 8
Dependent Claim
78 words
An improved bilirubin reference composition comprising in its non-biological component from about 40 to about 85 weight percent water, from about 15 to about 60 weight percent of at least one alkylene polyol having from 2 to 5 carbon atoms, the remainder being bilirubin and, optionally, other natural biological materials selected from a group consisting of blood serum, enzymes, metabolites, electrolytes, and hormones, characterized in that said composition possesses a pH of from about 8.3 to about 9.5.
Claim 9
Dependent Claim
78 words
An improved bilirubin reference composition comprising in its non-biological component from about 40 to about 85 weight percent water, from about 15 to about 60 weight percent of at least one alkylene polyol having from 2 to 5 carbon atoms, the remainder being bilirubin and, optionally, other natural biological materials selected from a group consisting of blood serum, enzymes, metabolites, electrolytes, and hormones, characterized in that said composition possesses a pH of from about 8.3 to about 9.5.
Claim 10
Dependent Claim
21 words
The composition of claim 7 further comprising a sulfhydryl compound in an amount sufficient to further enhance the stability of bilirubin.
Claim 11
Dependent Claim
30 words
The composition of claim 8 wherein said sulfhydryl compound is present in an amount such that the oxidation-reduction potential of said composition is from about -30 to about -300 millivolts.
Claim 12
Dependent Claim
44 words
The composition of claim 9 wherein said sulfhydryl compound is present in an amount such that the oxidation-reduction potential of said standard is from about -100 to about -200 millivolts and wherein said composition possesses a pH of from about 8.3 to about 8.7.
Claim 13
Dependent Claim
36 words
The composition of claim 10 wherein said sulfhydryl compound is present in an amount such that the oxidation-reduction potential of said standard is about -160 millivolts and wherein said composition possesses a pH of about 8.5.
Claim 14
Dependent Claim
28 words
The composition of claim 11 wherein said sulfhydryl compound is selected from a group consisting of dithioerythreitol, dithiothreitol, mercaptoethanol, cycsteine, reduced glutathione, N-acetyl cysteine, mercaptoacetate, and mixtures thereof.
Claim 15
Dependent Claim
11 words
The composition of claim 12 whrein said sulfhydryl compound is dithiothreitol.
Claim 16
Dependent Claim
47 words
The composition of claim 8 wherein bilirubin is present in an amount from about 0.1 to about 40 milligrams per deciliter and wherein said composition comprises from about 60 to about 80 weight percent and from about 20 to about 40 weight percent of said alkylene polyol.
Claim 17
Dependent Claim
22 words
The composition of claim 14 wherein said bilirubin is present in an amount from about 1 to about 30 milligrams per deciliter.
Claim 18
Dependent Claim
21 words
The composition of claim 15 wherein bilirubin is present in an amount from about 2 to about 25 milligrams per deciliter.
Claim 19
Independent Claim
45 words
A method for increasing the shelf-life of a blood serum bilirubin reference composition comprising adding to said composition a sulfhydryl compound in an amount sufficient to enhance the stability of bilirubin and adjusting the pH of said composition to from about 8.3 to about 9.5.
Claim 20
Independent Claim
45 words
A method for increasing the shelf-life of a blood serum bilirubin reference composition comprising adding to said composition a sulfhydryl compound in an amount sufficient to enhance the stability of bilirubin and adjusting the pH of said composition to from about 8.3 to about 9.5.
Claim 21
Dependent Claim
39 words
An improved blood serum reference composition of the type comprising blood serum having a bilirubin constituent of known value, characterized in that said composition further comprises a sulfhydryl compound in an amount sufficient to enhance the stability of bilirubin.
Claim 22
Dependent Claim
39 words
An improved blood serum reference composition of the type comprising blood serum having a bilirubin constituent of known value, characterized in that said composition further comprises a sulfhydryl compound in an amount sufficient to enhance the stability of bilirubin.
Claim 23
Dependent Claim
82 words
An improved bilirubin reference composition comprising in its non-biological component from about 40 to about 85 weight percent water, from about 15 to about 60 weight percent of at least one alkylene polyol having from 2 to 5 carbon atoms, the remainder being bilirubin and, optionally, other natural biological materials selected from a group consisting of blood serum, enzymes, metabolites, electrolytes, and hormones, characterized in that said composition further comprises a sulfhydryl compound in an amount to enhance the stability of bilirubin.
Claim 24
Dependent Claim
82 words
An improved bilirubin reference composition comprising in its non-biological component from about 40 to about 85 weight percent water, from about 15 to about 60 weight percent of at least one alkylene polyol having from 2 to 5 carbon atoms, the remainder being bilirubin and, optionally, other natural biological materials selected from a group consisting of blood serum, enzymes, metabolites, electrolytes, and hormones, characterized in that said composition further comprises a sulfhydryl compound in an amount to enhance the stability of bilirubin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-05-06 | 1983-11-05 | 1984-05-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-05-06 | 1987-11-05 | 1988-05-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-05-06 | 1991-11-05 | 1992-05-06 | $7700.00 | Window Closed / Lapsed |