US4200625A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilized binder for automated assay
Assignee: Becton Dickinson and Co
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Filing: 1978-07-12
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Granted: 1980-04-29
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Expires: 1998-07-12
Abstract
Abstract In an automated assay employing a reusable immobilized binder in a flow-through chamber, the binder is supported on a cotton support preferably mercerized absorbent cotton. Such cotton support prevents plugging when using free serum or plasma samples, and in addition, does not compress, has good flow properties, is easily handled, has low non-specific binding of proteins and steroids and is stable.
Inventors
- 👤 Max G. Reese
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 23:15
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
64 words
In an assay wherein a ligand to be assayed and labeled form of the ligand are caused to flow through a chamber having a binder specific for the ligand supported on a solid substrate, and wherein the binder is regenerated for reuse in the assay by releasing ligand and labeled ligand from the binder, the improvement comprising: employing fibrous cotton as said solid substrate.
Claim 2
Dependent Claim
64 words
In an assay wherein a ligand to be assayed and labeled form of the ligand are caused to flow through a chamber having a binder specific for the ligand supported on a solid substrate, and wherein the binder is regenerated for reuse in the assay by releasing ligand and labeled ligand from the binder, the improvement comprising: employing fibrous cotton as said solid substrate.
Claim 3
Dependent Claim
12 words
The assay of claim 1 wherein the cotton is an absorbent cotton.
Claim 4
Dependent Claim
13 words
The assay of claim 2 wherein the absorbent cotton is mercerized absorbent cotton.
Claim 5
Dependent Claim
15 words
The assay of claim 3 wherein the binder is covalently linked to the cotton support.
Claim 6
Dependent Claim
11 words
The assay of claim 4 wherein the labeled ligand is radiolabeled.
Claim 7
Dependent Claim
11 words
The assay of claim 5 wherein the binder is an antibody.
Claim 8
Dependent Claim
16 words
The assay of claim 5 wherein the ligand to be assayed is in a serum sample.
Claim 9
Dependent Claim
75 words
In a flow-through chamber for an assay for a ligand including a binder specific for the ligand supported on a solid substrate wherein the ligand to be assayed and labeled form of the ligand are caused to flow through the chamber and wherein the binder is regenerated for reuse in the assay by releasing ligand and the labeled form of the ligand from the binder, the improvement comprising: employing fibrous cotton as said solid substrate.
Claim 10
Dependent Claim
75 words
In a flow-through chamber for an assay for a ligand including a binder specific for the ligand supported on a solid substrate wherein the ligand to be assayed and labeled form of the ligand are caused to flow through the chamber and wherein the binder is regenerated for reuse in the assay by releasing ligand and the labeled form of the ligand from the binder, the improvement comprising: employing fibrous cotton as said solid substrate.
Claim 11
Dependent Claim
13 words
The flow-through chamber of claim 8 wherein the cotton is an absorbent cotton.
Claim 12
Dependent Claim
14 words
The flow-through chamber of claim 9 wherein the absorbent cotton is mercerized absorbent cotton.
Claim 13
Dependent Claim
12 words
The flow-through chamber of claim 10 wherein the binder is an antibody.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-04-29 | 1983-10-29 | 1984-04-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-04-29 | 1987-10-29 | 1988-04-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-04-29 | 1991-10-29 | 1992-04-29 | $7700.00 | Window Closed / Lapsed |