US4197291A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Hydrolytic enzyme material
Assignee: Individual
•
Filing: 1978-03-17
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Granted: 1980-04-08
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Expires: 1998-03-17
Abstract
Abstract Disclosure relates to novel hydrolytic enzyme product derived from the pineapple plant useful for the digestion, dissection and separation of non-viable, devitalized tissue, especially eschar tissue from viable tissue in a mammalian host.
Inventors
- 👤 Gerold K. V. Klein
- 👤 John C. Houck
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 08:07
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/63; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
102 words
An enzyme product obtainable from bromelain capable of debridement of devitalized tissue from a mammalian host which comprises a water soluble, heat labile protein free of caseinolytic activity having a peak isoelectric point of about 6, comprising at least two subunits, each subunit having a molecular weight of from about 14,300 to 15,000 daltons, with a characteristic absorption peak in the ultraviolet region of the spectrum at 280 nm.; the said product being active in the absence of sulfhydryl activation and in the presence of sulfhydryl deactivating quantities of phenylmercuric acetate, and the physiological acceptable alkali metal and acid addition salts thereof.
Claim 2
Dependent Claim
102 words
An enzyme product obtainable from bromelain capable of debridement of devitalized tissue from a mammalian host which comprises a water soluble, heat labile protein free of caseinolytic activity having a peak isoelectric point of about 6, comprising at least two subunits, each subunit having a molecular weight of from about 14,300 to 15,000 daltons, with a characteristic absorption peak in the ultraviolet region of the spectrum at 280 nm.; the said product being active in the absence of sulfhydryl activation and in the presence of sulfhydryl deactivating quantities of phenylmercuric acetate, and the physiological acceptable alkali metal and acid addition salts thereof.
Claim 3
Dependent Claim
112 words
A pharmaceutical composition comprising a pharmaceutically acceptable excipient together with an enzyme product obtainable from bromelain capable of debridement of devitalized tissue from a mammalian host which comprises a water soluble, heat labile protein, free of caseinolytic activity, having a peak isoelectric point at about 6 comprising at least 2 subunits, each subunit having a molecular weight of from about 14,300 to 15,000 daltons with a characteristic absorbtion peak in the ultraviolet region of the spectrum at 280 nm.; the said enzyme being active in the absence of sulfhydryl activation and in the presence of sulfhydryl deactivating quantities of phenylmercuric acetate, and the physiologically acceptable alkali metal and acid addition salts thereof.
Claim 4
Dependent Claim
112 words
A pharmaceutical composition comprising a pharmaceutically acceptable excipient together with an enzyme product obtainable from bromelain capable of debridement of devitalized tissue from a mammalian host which comprises a water soluble, heat labile protein, free of caseinolytic activity, having a peak isoelectric point at about 6 comprising at least 2 subunits, each subunit having a molecular weight of from about 14,300 to 15,000 daltons with a characteristic absorbtion peak in the ultraviolet region of the spectrum at 280 nm.; the said enzyme being active in the absence of sulfhydryl activation and in the presence of sulfhydryl deactivating quantities of phenylmercuric acetate, and the physiologically acceptable alkali metal and acid addition salts thereof.
Claim 5
Dependent Claim
12 words
A composition as in claim 2 wherein the excipient is physiological saline.
Claim 6
Dependent Claim
18 words
A composition as in claim 3 wherein the weight ratio of enzyme product to physiological saline is 1:1.
Claim 7
Dependent Claim
13 words
A composition as in claim 2 additionally containing at least one antimicrobial agent.
Claim 8
Dependent Claim
23 words
A composition as in claim 2 wherein the excipient is selected from the group consisting of solid polysaccharide gels and solid hydrocarbon bases.
Claim 9
Dependent Claim
14 words
A composition as in claim 6 wherein the polysaccharide gel is an agar gel.
Claim 10
Dependent Claim
12 words
A composition as in claim 6 wherein the hydrocarbon bases is petrolatum.
References Cited (Backward Citations)
US3409719A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-04-08 | 1983-10-08 | 1984-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-04-08 | 1987-10-08 | 1988-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-04-08 | 1991-10-08 | 1992-04-08 | $7700.00 | Window Closed / Lapsed |