US4195074A
Expired / Lapsed
US
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Process for producing a soluble rubella antigen
Assignee: Abbott Laboratories
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Filing: 1978-01-31
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Granted: 1980-03-25
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Expires: 1998-01-31
Abstract
Abstract Purified soluble antigen, specific for rubella virus, is isolated from growth media of rubella-infected cell cultures by affinity and gel permeation chromatography and characterized, inter alia, by its specific activity. Antigen-sensitized particles are employed as immunoassay reagents in, for example, agglutination assays for detection and quantification of rubella antibodies in body fluids such as serum, spinal fluid and the like.
Inventors
- 👤 John W. Safford, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 08:36
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/20; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; C; C12; C12N; C12N2770/00; C12N2770/00011; C12N2770/36011; C12N2770/36211; C12N2770/36234; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S530/00; Y10S530/806; Y10S530/81; Y10S530/82; Y10S530/826
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
150 words
A process for producing a soluble rubella antigen, said process comprising, in sequence: (a) subjecting growth media of a tissue culture of rubella virus infected cells to chromatographic separation through a column consisting of a solid phase to which IgG, derived from human serum known to contain antibodies reactive with rubella antigens, has been convalently coupled; (b) eluting from said column the rubella antigen materials bound to the IgG therein with a buffer having a pH of about 12; (c) neutralizing the rubella antigen materials to a pH of about 8; (d) subjecting the separated eluted rubella antigen materials to gel permeation chromatography to achieve material separation on the basis of molecular size; (e) collecting the fractions containing said materials having a molecular weight of about 40,000 to about 60,000 daltons; (f) isolating rubella antigen from said sized, separated eluted materials on the basis of relative reverse passive hemagglutination activity.
Claim 2
Dependent Claim
150 words
A process for producing a soluble rubella antigen, said process comprising, in sequence: (a) subjecting growth media of a tissue culture of rubella virus infected cells to chromatographic separation through a column consisting of a solid phase to which IgG, derived from human serum known to contain antibodies reactive with rubella antigens, has been convalently coupled; (b) eluting from said column the rubella antigen materials bound to the IgG therein with a buffer having a pH of about 12; (c) neutralizing the rubella antigen materials to a pH of about 8; (d) subjecting the separated eluted rubella antigen materials to gel permeation chromatography to achieve material separation on the basis of molecular size; (e) collecting the fractions containing said materials having a molecular weight of about 40,000 to about 60,000 daltons; (f) isolating rubella antigen from said sized, separated eluted materials on the basis of relative reverse passive hemagglutination activity.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein, in step (a), the solid phase consists of agarose beads.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-03-25 | 1983-09-24 | 1984-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-03-25 | 1987-09-24 | 1988-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-03-25 | 1991-09-24 | 1992-03-25 | $7700.00 | Window Closed / Lapsed |