US4195072A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Stabilized platelet factor 4 immunoassay standards
Assignee: Abbott Laboratories
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Filing: 1978-07-05
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Granted: 1980-03-25
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Expires: 1998-07-05
Abstract
Abstract The present invention encompasses a stabilized platelet factor 4 immunoassay standard comprising an aqueous solution containing 10 to 100 ng/ml of platelet factor 4, carrier protein, and an effective stabilizing amount of heparin, as well as 125 I labeled platelet factor 4 reagent stabilized in the same manner. These reagents are useful for testing blood plasma for activation of the coagulation system.
Inventors
- 👤 Erwin F. Workman, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 15:32
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/86; G01N33/96; G01N2400/00; G01N2400/10; G01N2400/38; G01N2400/40; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/826
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
30 words
A stabilized platelet factor 4 immunoassay standard comprising an aqueous solution containing 10 to 100 ng/ml of platelet factor 4, carrier protein and an effective stabilizing amount of heparin activity.
Claim 2
Dependent Claim
30 words
A stabilized platelet factor 4 immunoassay standard comprising an aqueous solution containing 10 to 100 ng/ml of platelet factor 4, carrier protein and an effective stabilizing amount of heparin activity.
Claim 3
Dependent Claim
36 words
A stabilized platelet factor 4 immunoassay standard comprising a buffered diluent containing 10 to 100 ng/ml of platelet factor 4, carrier protein, an effective antibacterial amount of preservative and an effective stabilizing amount of heparin activity.
Claim 4
Dependent Claim
36 words
A stabilized platelet factor 4 immunoassay standard comprising a buffered diluent containing 10 to 100 ng/ml of platelet factor 4, carrier protein, an effective antibacterial amount of preservative and an effective stabilizing amount of heparin activity.
Claim 5
Dependent Claim
38 words
A stabilized 125 I labeled platelet factor 4 immunoassay reagent comprising an aqueous solution containing 125 I labeled platelet factor 4 having up to 0.45μ Ci/ml of radioactivity, carrier protein and an effective stabilizing amount of heparin activity.
Claim 6
Dependent Claim
38 words
A stabilized 125 I labeled platelet factor 4 immunoassay reagent comprising an aqueous solution containing 125 I labeled platelet factor 4 having up to 0.45μ Ci/ml of radioactivity, carrier protein and an effective stabilizing amount of heparin activity.
Claim 7
Dependent Claim
44 words
A stabilized 125 I labeled platelet factor 4 immunoassay reagent comprising a buffered diluent containing 125 I labeled platelet factor 4 having up to 0.45μ Ci/ml of radioactivity, carrier protein, an effective antibacterial amount of preservative and an effective stabilizing amount of heparin activity.
Claim 8
Dependent Claim
44 words
A stabilized 125 I labeled platelet factor 4 immunoassay reagent comprising a buffered diluent containing 125 I labeled platelet factor 4 having up to 0.45μ Ci/ml of radioactivity, carrier protein, an effective antibacterial amount of preservative and an effective stabilizing amount of heparin activity.
Claim 9
Dependent Claim
85 words
In a radioimmunoassay method for platelet factor 4 in blood plasma wherein concentrations of platelet factor 4 in standards are used to derive concentrations of platelet factor 4 in blood plasma by competitive binding of 125 I labeled platelet factor 4 and platelet factor 4 from test samples with platelet factor 4 antiserum, followed by precipitation and counting of bound 125 I labeled platelet factor 4, the improvement comprising using heparin stabilized platelet factor 4 standards and heparin stabilized 125 I labeled platelet factor 4.
Claim 10
Dependent Claim
85 words
In a radioimmunoassay method for platelet factor 4 in blood plasma wherein concentrations of platelet factor 4 in standards are used to derive concentrations of platelet factor 4 in blood plasma by competitive binding of 125 I labeled platelet factor 4 and platelet factor 4 from test samples with platelet factor 4 antiserum, followed by precipitation and counting of bound 125 I labeled platelet factor 4, the improvement comprising using heparin stabilized platelet factor 4 standards and heparin stabilized 125 I labeled platelet factor 4.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-03-25 | 1983-09-24 | 1984-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-03-25 | 1987-09-24 | 1988-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-03-25 | 1991-09-24 | 1992-03-25 | $7700.00 | Window Closed / Lapsed |