US4191808A
Expired / Lapsed
US
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Method of measuring dipeptidyl-amino-peptidase activity
Assignee: Ajinomoto Co Inc
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Filing: 1978-04-17
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Granted: 1980-03-04
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Expires: 1998-04-17
Abstract
Abstract The activity of X-prolyl dipeptidyl-aminopeptidase against X-L-proline-Y wherein X is an amino acid residue and Y is p-nitroaniline, p-phenylazoaniline or 4-phenylazo-1-naphthylamine is used as a measure of human afflictions such as hepatobiliary diseases and carcinomas.
Inventors
- 👤 Toshiharu Nagatsu
- 👤 Shumpei Sakakibara
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 07:46
CPC Classifications
C; C07; C07K; C07K5/00; C07K5/04; C07K5/06; C07K5/06104; C07K5/06113; C07D; C07D207/00; C07D207/02; C07D207/04; C07D207/10; C07D207/16; C07K5/06008; C07K5/06017; C07K5/06026; C07K5/06086; C07K5/06095; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; A; A61; A61K; A61K38/00; C12Q2337/00; C12Q2337/10; C12Q2337/12; C12Q2337/30
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
99 words
A method of measuring X-prolyl dipeptidyl-aminopeptidase activity which comprises contacting a compound having the structure: X-L-proline-Y or an acid salt thereof with said X-prolyl dipeptidyl-aminopeptidase in an aqueous medium at pH 7 to 9 under conditions suitable for the enzymatic reaction to occur and assaying the enzymatically liberated Y-H, said X being a residue of an amino acid selected from the group consisting of glycine, alanine, valine, leucine, serine, threonine, phenylalanine, tyrosine, tryptophane, glutamic acid, aspartic acid, lysine and arginine; and said Y being a residue of said Y-H selected from the group consisting of p-nitroaniline, p-phenylazoaniline, and 4-phenylazo-1-naphthylanine.
Claim 2
Independent Claim
99 words
A method of measuring X-prolyl dipeptidyl-aminopeptidase activity which comprises contacting a compound having the structure: X-L-proline-Y or an acid salt thereof with said X-prolyl dipeptidyl-aminopeptidase in an aqueous medium at pH 7 to 9 under conditions suitable for the enzymatic reaction to occur and assaying the enzymatically liberated Y-H, said X being a residue of an amino acid selected from the group consisting of glycine, alanine, valine, leucine, serine, threonine, phenylalanine, tyrosine, tryptophane, glutamic acid, aspartic acid, lysine and arginine; and said Y being a residue of said Y-H selected from the group consisting of p-nitroaniline, p-phenylazoaniline, and 4-phenylazo-1-naphthylanine.
Claim 3
Dependent Claim
20 words
A method as set forth in claim 1, wherein human serum is used as the source of said X-prolyl dipeptidyl-aminopeptidase.
Claim 4
Dependent Claim
15 words
A method as set forth in claim 2 wherein the said compound is glycyl-L-proline p-nitroanilide.
Claim 5
Dependent Claim
16 words
A method as set forth in claim 2 wherein the said compound is glycyl-L-proline p-nitroanilide tosylate.
Claim 6
Dependent Claim
15 words
A method as set forth in claim 2 wherein the said compound is L-lysyl-L-proline p-nitroanilide.
Claim 7
Dependent Claim
15 words
A method as set forth in claim 2 wherein the said compound is L-arginyl-L-proline p-nitroanilide.
Claim 8
Dependent Claim
15 words
A method as set forth in claim 2 wherein the said compound is L-alanyl-L-proline p-nitroanilide.
Claim 9
Dependent Claim
15 words
A method as set forth in claim 2 wherein the said compound is L-glutamyl-L-proline p-nitroanilide.
Claim 10
Dependent Claim
15 words
A method as set forth in claim 2 wherein the said compound is L-aspartyl-L-proline p-nitroanilide.
Claim 11
Independent Claim
119 words
A method for detecting a human disease which disease is characterized by elevated levels of X-prolyl dipeptidyl-aminopeptidase in human serum which comprises measuring the activity of said X-prolyl dipetidyl-aminopeptidase in human serum by contacting a compound having the structure: X-L-proline-Y or an acid salt thereof with said human serum in an aqueous medium at from 30° to 45° C. at a pH of from 7.5 to 8.9; and assaying enzymatically liberated Y-H; said X being an amino acid selected from the group consisting of glycine, alanine, valine, leucine, serine, threonine, phenylalanine, tyrosine, tryptophane, glutamic acid, aspartic acid, lysine and arginine; and said Y being a residue of said Y-H selected from the group consisting of p-nitroaniline, p-phenylazoaniline, and 4-phenylazo-1-naphthylanine.
Claim 12
Independent Claim
119 words
A method for detecting a human disease which disease is characterized by elevated levels of X-prolyl dipeptidyl-aminopeptidase in human serum which comprises measuring the activity of said X-prolyl dipetidyl-aminopeptidase in human serum by contacting a compound having the structure: X-L-proline-Y or an acid salt thereof with said human serum in an aqueous medium at from 30° to 45° C. at a pH of from 7.5 to 8.9; and assaying enzymatically liberated Y-H; said X being an amino acid selected from the group consisting of glycine, alanine, valine, leucine, serine, threonine, phenylalanine, tyrosine, tryptophane, glutamic acid, aspartic acid, lysine and arginine; and said Y being a residue of said Y-H selected from the group consisting of p-nitroaniline, p-phenylazoaniline, and 4-phenylazo-1-naphthylanine.
Claim 13
Dependent Claim
15 words
A method as set forth in claim 10 wherein the said compound is glycyl-L-proline p-nitroanilide.
Claim 14
Dependent Claim
16 words
A method as set forth in claim 10 wherein the said compound is glycyl-L-proline p-nitroanilide tosylate.
Claim 15
Dependent Claim
15 words
A method as set forth in claim 10 wherein the said compound is L-lysyl-L-proline p-nitroanilide.
Claim 16
Dependent Claim
15 words
A method as set forth in claim 10 wherein the said compound is L-arginyl-L-proline p-nitroanilide.
Claim 17
Dependent Claim
15 words
A method as set forth in claim 10 wherein the said compound is L-alanyl-L-proline p-nitroanilide.
Claim 18
Dependent Claim
15 words
A method as set forth in claim 10 wherein the said compound is L-glutamyl-L-proline p-nitroanilide.
Claim 19
Dependent Claim
15 words
A method as set forth in claim 10 wherein the said compound is L-aspartyl-L-proline p-nitroanilide.
Claim 20
Dependent Claim
14 words
A method as set forth in claim 10, wherein said disease is malignant tumor.
Claim 21
Dependent Claim
31 words
A ,ethod as set forth in claim 10, wherein said disease is a disease selected from the group consisting of gastric cancer, pancreatic cancer, pulmonary cancer, leukemia, lymphosarcoma and Hodgkin's disease.
Claim 22
Dependent Claim
16 words
A method as set forth in claim 10, said disease is hepatobiliary disease or essential hypertension.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-03-04 | 1983-09-03 | 1984-03-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-03-04 | 1987-09-03 | 1988-03-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-03-04 | 1991-09-03 | 1992-03-04 | $7700.00 | Window Closed / Lapsed |